A simple handling technique for mammalian oocytes and embryos during preparation for transmission electron microscopy

Due to their small size, mammalian oocytes and embryos pose unique problems during preparation for transmission electron microscopy. This paper outlines a method which combines protein embedding with centrifugation to locate the specimens on the face of a Beem capsule mould. This method facilitates both the processing of oocytes with minimal loss and rapid location of the specimens within the block for simultaneous sectioning, staining and examination.     

乙烯氧化制环氧乙烷固定床反应器的模拟及稳定性分析

在筛选出合适的乙烯氧化制环氧乙烷动力学方程的基础上,对工业固定床氧化反应器进行一维和二维模型的模拟计算。考察操作工艺参数对反应器稳定性、转化率影响的敏感性以及N_2和CH_4做致稳气时反应温度和转化率的轴向、径向分布。     

Comparison of the glucose-lowering properties of vanadyl sulfate and bis(maltolato)oxovanadium(IV) following acute and chronic administration

The possibility that progesterone or estradiol may regulate expression of G protein in the rat myometrium during the course of pregnancy has been investigated using 1) immunoblot analysis of Gi2 alpha, Gi3 alpha, and Gq alpha subunits and 2) hybridization blot analysis of subunit mRNA. Eighteen hours after administration, estradiol had significantly increased the levels of both Gi2 alpha subunit and Gi2 alpha mRNA (by 40% and 32%, respectively). In control pregnant rats, we observed similar changes at the end of pregnancy, when myometrial concentrations of estradiol had increased, i.e., a 41% increase in immunoreactive Gi2 alpha subunit that correlated with a parallel 45% increase in mRNA levels. In contrast, levels of immunoreactive Gi3 alpha subunit and mRNA, which decreased with advancing gestation, were not influenced by estradiol or progesterone administration. Progesterone administration resulted 30 h later in a significantly decreased level of Gq alpha immunoreactivity (32%) and Gq alpha mRNA (30%). In control rats, Gq alpha protein and mRNA were also significantly lower at midpregnancy under progesterone dominance vs. term. At this stage, a twofold increase in Gq alpha subunit correlated with a 40% increase in mRNA levels. These results demonstrate that myometrial Gi2 alpha and Gq alpha subunits are physiological targets for estradiol and progesterone, respectively, in vivo. Alterations of these G protein levels are discussed in relation to their mediating effects on adenylyl cyclase activity or the phospholipase C pathway during the course of pregnancy.     

Tight bounds on Rician-type error probabilities and someapplications

Consider the classic problem of evaluating the probability that one Rician random variable exceeds another, possibly correlated, Rician random variable. This probability is given by Stein (1964) in terms of the Marcum's Q-function, which requires numerical integration on the computer for its evaluation. To facilitate application in many digital communication problems, we derive here tight upper and lower bounds on this probability. The bounds are motivated by a classic result in communication theory, namely, the error probability performance of binary orthogonal signaling over the Gaussian channel with unknown carrier phase. Various applications of the bounds are reported, including the evaluation of the bit error probabilities of MDPSK and MPSK with differential detection and generalized differential detection, respectively. The bounds prove to be tight in all cases. Further applications will be reported in the future     

Numerical simulation model of vibration responses of rectangular plates embedded with piezoelectric actuators

A numerical simulation model for random large amplitude vibration control of composite plate using piezoelectric material is presented. The H_∞ control design is employed to suppress the large amplitude vibrations of composites plates under random loading. The numerical simulation model is developed and based on the finite element method. The finite element governing equation includes fully coupled structural and electrical nodal degrees of freedom, and consider the von Karman large amplitude vibration. The modal reduction method using the structural modes is adopted to reduce the finite element equations into a set of modal equations with fewer degrees of freedom. The modal equations are then employed for controller design and time domain simulation. In the simulations without control, the value of the linear mode to the nonlinear deflection is quantified; and the minimum number of linear modes needed for accurate model is obtained. In the simulations with control, it is shown that the truncated modes, which are neglected in the control design, deteriorate the controller performance. Generally, the vibration reduction level is not monotonically increasing with the size of the piezoelectric actuator. The optimal piezoelectric actuator size depends on the excitation level. For higher excitation level, optimal actuator size is larger. The H_∞ controller based on the linear finite element formulation gives better vibration reduction for small amplitude vibration, but it still gives reasonable performance for large amplitude vibration provided that the piezoelectric actuator is big and powerful enough.     

Asymptotic performance analysis of ESPRIT, higher order ESPRIT, andvirtual ESPRIT algorithms

In this paper, we present an asymptotic performance analysis of three subspace-based methods for direction of arrival (DOA) estimation-the ESPRIT algorithm using second order statistics, the higher order ESPRIT algorithm using fourth-order cumulants, and the virtual ESPRIT (VESPA) algorithm using fourth-order cumulants. We examine the least-squares version of these algorithms, derive the expressions for the asymptotic variance of the estimated DOAs, and use specific examples to compare the relative performance of the algorithms. Finally, we present Monte Carlo simulations to validate the theoretical analysis     

Endothelium-dependent effect of perindopril and enalaprilat in rat thoracic aorta

AIM: To study the effect of the angiotensin-converting enzyme (ACE) inhibitors perindopril (Per) and enalaprilat (Ena) on the reactivity of the endothelium in normal rats. METHODS: Male rats were treated intragastrically with Per (2 mg.kg-1.d-1) or placebo (n = 18) for 6 wk. Aorta was isolated for experiment. Another set of isolated aortic rings with and without endothelium were incubated with Ena (0.1 mumol.L-1) for 30 min. Responses to acetylcholine, serotonin, phenylephrine, sodium nitroprusside (SN), and nitroglycerin (Nit) were observed. RESULTS: Endothelium-dependent relaxation to acetylcholine was augmented in aortic rings from rats treated with Per in comparison with control. The IC50 value (95% confidence limits) decreased from 3.8 (0.56-26.1) mumol.L-1 (control group) to 0.98 (0.28-3.41) mumol.L-1 (Per-treated group). The maximal relaxation was augmented from 62 +/- 9% to 78 +/- 10% (P < 0.01). However, the responses to the endothelium-independent vasodilators, SN and Nit, were similar. Serotonin- and phenylephrine-induced contractions were decreased, which were influenced by basal release of endothelium-derived relaxing factor (EDRF). EC50 values was 6.1 (2.6-14.4) nmol.L-1 vs 8.3 (3.6-18.8) nmol.L-1 in comparison with control group and Per-treated group. The maximal contraction was decreased from 2.42 +/- 0.29 g (control group) to 1.96 +/- 0.25 g (treated group) (P < 0.01). Similar results were found in incubation with Ena. CONCLUSION: Ena and Per enhanced the basic release of EDRF from vascular endothelium.