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排序方式: 共有69条查询结果,搜索用时 15 毫秒
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Clotilde LM Bernard C Sequera DE Karmali A Fusellier A Carter JM 《Journal of laboratory automation》2012,17(4):309-314
Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates. 相似文献
3.
Clotilde Muller Sandrine Muller Aicha Sissoko Alexandre Klein Bernadette Faller Francois Chantrel 《Hemodialysis international. International Symposium on Home Hemodialysis》2012,16(4):556-558
Lanthanum therapy is an efficient therapy of hyperphosphoremia by chelating phosphore in the digestive tract. Lanthanum is a silvery white metallic element that belongs to group 3 of the periodic table. This drug is lightly absorbed and has low water solubility. It should be borne in mind that abdominal X‐rays of patients taking lanthanum carbonate may have a radio‐opaque appearance typical of imaging agents. This characteristic is suggested to confirm adherence of the patient by doing an abdominal X‐ray. We describe in our case a particular good compliant patient with slow digestive transit, which ends by pseudo‐occlusion. 相似文献
4.
Clotilde Minfray Thierry Le Mogne Jean-Michel Martin Tasuku Onodera Sayaka Nara Shuko Takahashi 《摩擦学汇刊》2013,56(5):589-601
Zinc phosphate glass is considered to be the main constituent of tribofilms generated under boundary lubrication with zinc dialkyldithiophosphate (ZDDP), a well-known antiwear additive. The reaction occurring during friction between zinc phosphate glasses and steel native iron oxide layer is investigated by both an experimental approach and by Molecular Dynamics simulations (MD). The importance of this “tribochemical” reaction in the general ZDDP antiwear process is discussed. 相似文献
5.
Paule Njiwa Clotilde Minfray Thierry Le Mogne Béatrice Vacher Jean-Michel Martin Shigeki Matsui Masaru Mishina 《Tribology Letters》2011,44(1):19-30
In this study, we are interested in the anti-wear properties of zinc dialkyl phosphate additive (ZP) in comparison with ‘classical’ zinc dialkyldithiophosphate (ZDDP). Friction tests were performed on a reciprocating tribometer using both ball-on-flat and cylinder-on-flat configurations
under a Hertzian contact pressure of 0.9 GPa. Experiments were carried out as a function of temperature (25 and 100 °C), sliding
speed (25, 50 and 100 mm/s) and additives concentrations. Ball wear scar diameters as well as friction coefficient were measured.
In order to better understand the anti-wear mechanisms of these additives, friction tests were followed by surface analyses
such as AES (Auger Electron Spectroscopy) and XPS (X-Ray Photoelectron Spectroscopy). Transmission Electron Microscopy (TEM)
observations of the ZDDP and ZP tribofilms were also carried out to visualise the generated layers. The anti-wear capability of ZP molecule is discussed. 相似文献
6.
通过与其它传感技术的对比,简要分析了激光视觉在焊接生产中得到成功应用的原因。分析了早期激光视觉系统的特征和当时面临的挑战。介绍了Servo-Robot激光视觉的关键技术和已取得的显著进步,阐述了集成激光视觉的移动式便携焊接机器人和激光视觉在焊缝质量检测中的应用。展望了激光视觉系统的未来和智能视觉在提高机器人性能方面发展的显著进步,包括改进机器人人工示教、离线编程及视觉辅助机器人示教。 相似文献
7.
The paper was aimed at modelling hydrodynamics in lab-scale polymer electrolyte fuel cells, more particularly in the distribution plates. Residence time distributions (RTD) were measured in a wide range of flow rates, using UV absorption of ozone injected as a tracer in the cell. The volume of the inlet and outlet systems, being of the order of the volume of the gas circuit machined in the distribution plates, was taken into account in calculations. Hydrodynamic behaviour of two flow patterns of the commercialised fuel cell investigated was successfully described using ideal reactor models. 相似文献
8.
Florie VidbergAuthor Vitae Rabah ZeggariAuthor Vitae Christian PieralliAuthor Vitae Clotilde Amiot Christophe RouxAuthor Vitae Bruno WacogneAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(1):19-25
We present a simple optical micro-system used to measure the transmission spectra of oocytes in order to qualify their maturation stage. Two applications of the device are possible: (i) the evaluation of the maturation stages of oocytes, and (ii) the development of a fertilization indicator. For the first application, GV, MI and MII oocytes were also analysed. Transmission spectra allow the 3 maturation stages to be identified but cannot be used to estimate the maturity of an unknown oocyte. Oocytes are subject to continuous development. This is why spectral separation of the 3 maturation stages cannot be made although they can be visually identified. However, the visual observation remains biologist-dependent. We therefore investigated the temporal maturation evolution of the oocytes in terms of transmission spectra and probability analysis. Results show that oocytes to be fertilized should not only be chosen in the MII stage, but also at the right time during the MII stage. This particular aspect requires further investigation. However, spectral measurements could be used as a technique for monitoring the maturation evolution of the oocytes. Fertilized oocytes exhibiting fertilization abnormalities were also tested. The device proved to be an efficient fertilization indicator. 相似文献
9.
M Kabir SW Rizkalla A Quignard-Boulangé M Guerre-Millo J Boillot B Ardouin J Luo G Slama 《Canadian Metallurgical Quarterly》1998,128(11):1878-1883
The of this study was to evaluate the chronic effects of a high (waxy corn) vs. a low (mung beans) glycemic index starch diet on the lipogenic enzymes, fatty acid synthase (FAS) and lipoprotein lipase (LPL). Normal and diabetic (streptozotocin-injected on d 2 of life) male Sprague-Dawley rats consumed a diet containing 575 g/kg carbohydrates either as waxy cornstarch (WCS) or as mung bean starch (MBS). After 3 wk, neither body weights nor relative epididymal fat pad weights differed. In diabetic rats, the WCS diet induced high basal plasma insulin levels. Plasma triglycerides were not significantly affected by diet in either normal or diabetic rats. Adipose tissue and liver LPL activities were not modified by the type of starch in the diet. In normal rats, FAS activity and gene expression in epididymal adipose tissue but not in liver were greater in rats consuming the WCS diet than in those consuming MBS. To evaluate the implication of insulin in this regulation, two genes regulated by insulin [GLUT4 and phosphoenolpyruvate carboxykinase (PEPCK)] were also studied. The high glycemic index WCS diet compared with the low glycemic index MBS diet resulted in lower hepatic PEPCK mRNA in both normal and diabetic rats. Normal, but not diabetic rats fed WCS had greater GLUT4 gene expression in adipocytes than did those fed MBS. We conclude that the total replacement of 575 g/kg low glycemic index starch by a high glycemic index starch for 3 wk caused the following in normal rats: 1) high FAS activity and mRNA in adipose tissue but not in liver and 2) high GLUT4 gene expression in adipose tissue. In both normal and diabetic rats this same diet resulted in lower hepatic PEPCK mRNA. Therefore, high glycemic index starch diet is implicated in stimulating FAS activity and lipogenesis and might have undesirable long-term metabolic effects. 相似文献
10.
Hannah C. Nerl Anuj Pokle Lewys Jones Knut Müller‐Caspary Karel H. W. van den Bos Clive Downing Eoin K. McCarthy Nicolas Gauquelin Quentin M. Ramasse Ivan Lobato Dermot Daly Juan Carlos Idrobo Sandra Van Aert Gustaaf Van Tendeloo Stefano Sanvito Jonathan N. Coleman Clotilde S. Cucinotta Valeria Nicolosi 《Advanced functional materials》2019,29(37)
The fabrication of 2D systems for electronic devices is not straightforward, with top‐down low‐yield methods often employed leading to irregular nanostructures and lower quality devices. Here, a simple and reproducible method to trigger self‐assembly of arrays of high aspect‐ratio chiral copper heterostructures templated by the structural anisotropy in black phosphorus (BP) nanosheets is presented. Using quantitative atomic resolution aberration‐corrected scanning transmission electron microscopy imaging, in situ heating transmission electron microscopy and electron energy‐loss spectroscopy arrays of heterostructures forming at speeds exceeding 100 nm s?1 and displaying long‐range order over micrometers are observed. The controlled instigation of the self‐assembly of the Cu heterostructures embedded in BP is achieved using conventional electron beam lithography combined with site specific placement of Cu nanoparticles. Density functional theory calculations are used to investigate the atomic structure and suggest a metallic nature of the Cu heterostructures grown in BP. The findings of this new hybrid material with unique dimensionality, chirality, and metallic nature and its triggered self‐assembly open new and exciting opportunities for next generation, self‐assembling devices. 相似文献