Design and characterization of a chitosan physical gel promoting wound healing in mice

In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical–chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.     

Collaborative virtual reality platform for visualizing space data and mission planning

This paper presents the system architecture of a collaborative virtual environment in which distributed multidisciplinary teams involved in space exploration activities come together and explore areas of scientific interest of a planet for future missions. The aim is to reduce the current challenges of distributed scientific and engineering meetings that prevent the exploitation of their collaborative potential, as, at present, expertise, tools and datasets are fragmented. This paper investigates the functional characteristics of a software framework that addresses these challenges following the design science research methodology in the context of the space industry and research. An implementation of the proposed architecture and a validation process with end users, based on the execution of different use cases, are described. These use cases cover relevant aspects of real science analysis and operation, including planetary data visualization, as the system aims at being used in future European missions. This validation suggests that the system has the potential to enhance the way space scientists will conduct space science research in the future.

     

Quality and accreditation in nephrology. The Italian perspective. Management and quality control

Three cases of extracranial vertebral artery dissections with upper limb peripheral motor deficit (C5-C6) are reported. Six similar cases were also found in the literature. Central neurological symptoms occurred in five of these nine cases, suggesting the diagnosis of dissection. The peripheral motor or sensorial deficit was strictly isolated in the four other cases, simulating radicular neuralgia due to discopathy or foraminal compression. In case of dissections, a precise analysis of pain is helpful to guide diagnosis; sharp, unbearable, continuous and extended neck pain without nocturnal paroxysms and posterior neck stiffness is typical. Analgesics or anti-inflammatory drugs are ineffective. Peripheral motor deficit is more common than sensory deficit. Recovery was complete in this series. In most cases, the radiculopathy appears to be due to cervical root compression in its extraforaminal course due to the dissection hematoma and rarely to radicular ischemia.     

Non-dimensional analysis for rock slope plane failure in seismic (pseudostatic) conditions

Bulletin of Engineering Geology and the Environment - This study examines plane failure analysis of rock slopes and discusses its effect on seismic actions in a non-dimensional form, incorporating...     

Histology as a valid and reliable tool to differentiate fresh from frozen-thawed fish

Selling fish products as fresh when they have actually been frozen and thawed is a common fraudulent practice in seafood retailing. Unlike fish products frozen to protect them against degenerative changes during transportation and to extend the product's storage life, fish intended for raw consumption in European countries must be previously frozen at -20° C for at least 24 h to kill parasites. The aim of this study was to use histological analysis to distinguish between fresh and frozen-thawed fish and to evaluate this method for use as a routine screening technique in compliance with the requirements of European Commission Regulation No. 882/2004 on official food and feed controls. Method performance (i.e., accuracy and precision) was evaluated on tissue samples from three common Mediterranean fish species; the evaluation was subsequently extended to include samples from 35 fish species in a second experiment to test for method robustness. Method accuracy was tested by comparing histological results against a "gold standard" obtained from the analysis of frozen and unfrozen fish samples prepared for the study. Method precision was evaluated according to interrater agreement (i.e., three laboratories with expertise in histopathology in the first experiment and three expert analysts in the second experiment) by estimating Cohen's kappa (and corresponding 95 % confidence intervals) for each pair of laboratories and experts and the combined Cohen's kappa for all three experts and laboratories. The observed interrater agreement among the three laboratories and the three experts indicated high levels of method accuracy and precision (high sensitivity and specificity) and method reproducibility. Our results suggest that histology is a rapid, simple, and highly accurate method for distinguishing between fresh and frozen-thawed fish, regardless of the fish species analyzed.     

An efficient procedure for mining egocentric temporal motifs

Data Mining and Knowledge Discovery - Temporal graphs are structures which model relational data between entities that change over time. Due to the complex structure of data, mining statistically...     

Parameters predictive of Legionella contamination in hot water systems: association with trace elements and heterotrophic plate counts

The contamination of hot water samples with Legionella spp. was studied in relation to temperature, total hardness, trace element concentrations (iron, zinc, manganese, and copper) and heterotrophic plate counts (HPC) at both 22 and 37 °C. Factor analysis and receiver operating characteristic (ROC) curves were used to establish the cut-off of water parameters as predictors for Legionella contamination. Legionella spp. was isolated in 194 out of 408 samples (47.5%), with Legionella pneumophila being the most common (92.8%). After multiple logistic regression analysis, the risk for legionellae colonisation was positively associated with Mn levels >6 μg l⁻¹, HPC at 22 °C >27 CFU l⁻¹, and negatively with temperature >55 °C and Cu levels >50 μg l⁻¹. Multiple regression analysis revealed that Legionella spp. counts were positively associated with Mn, HPC at 37 °C and Zn and negatively associated with temperature. Only 1 out of the 97 samples (1%) having a Mn concentration, an HPC at 22 °C and an HPC at 37 °C below the respective median values exhibited a Legionella spp. concentration exceeding 10⁴ CFU l⁻¹vs. 41 out of the 89 samples (46.1%) with the three parameters above the medians. Our results show a qualitative and quantitative relationship between Legionella spp., the Mn concentration and heterotrophic plate counts in hot water samples from different buildings, suggesting that these parameters should be included in a water safety plan. The role of manganese in biofilm formation and its possible involvement in the mechanisms favouring Legionella survival and growth in water niches should be investigated further.     

Mechanical and drug delivery properties of a chitosan–tartaric acid hydrogel suitable for biomedical applications

The present investigation describes the synthesis and the characterization of a novel highly stable polysaccharidic hydrogel system, designed for modified drug delivery. Gels and films based on the biodegradable and biocompatible chitosan (CS) were prepared by a crosslinking reaction between the polysaccharide amino groups with tartaric acid, using the short‐range crosslinkers 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) as coupling agents. The aim of the study is to characterize the novel CS hydrogel by means of rheological and mechanical measurements; in vitro swelling, release, and degradation studies were also carried out. Obtained results show how the structure of the obtained networks can deeply affect dynamomechanical properties of the hydrogel as well as the delivery rate of loaded model drugs. The mechanical characterization of the hydrogel, in the form of films, indicates that the film elasticity increases as the water content in the hydrogel increases. Rheological studies evidenced that the different network structures can affect the elastic modulus of the system. Release studies of two model molecules, i.e., vitamin B12 and blue dextran, of different steric hindrance were carried out using both the bulk gel and the film. In vitro release of both drugs was evaluated in water and in Hepes to assess the suitability of this novel drug delivery system for pharmaceutical applications. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011