Synthesis of Benzophenones and in vitro Evaluation of Their Anticancer Potential in Breast and Prostate Cancer Cells

Breast and prostate cancers are frequently treated with chemotherapy. Several novel chemicals are being reported for this purpose, particularly synthetic and natural benzophenones. This work reports the synthesis of substituted 2-hydroxybenzophenones through 1,4-conjugate addition/intramolecular cycloaddition/dehydration of nitromethane on key intermediate chromones. Structures were extensively studied by means of 2D NMR spectroscopy and single-crystal XRD. Their cytotoxicity was evaluated in vitro in two breast cancer cell lines (MDA-MB-231 and T47-D) and one prostate cancer cell line (PC3). The most potent compound exhibited good cytotoxic effects against the three cancer cell lines (IC₅₀ values ranging from 12.09 to 26.49 μm ) and induced cell-cycle retardation only on prostate cancer cells, which suggested that it might exert cell-type-specific effects.     

Coupled common fixed point theorems for a pair of commuting mappings in partially ordered complete metric spaces

The purpose of this paper is to establish a coupled coincidence point for a pair of commuting mappings in partially ordered complete metric spaces. We also present a result on the existence and uniqueness of coupled common fixed points. An example is given to support the usability of our results.     

Development of an empirical model for determining G*/sin δ in crumb rubber modified binders

This paper describes the development of an empirical model which may be used for predicting the G*/sin δ for neat and crumb rubber modified (CRM) binders. The model was developed using 36 unique CRM binder combinations, crumb rubber concentrations were varied at 5% intervals between 5% and 20%. The effects of crumb rubber particle size on model accuracy were also studied, ultimately a model was produced with the capability of predicting G*/sin δ values over a range of temperatures and crumb rubber concentrations. By definition, the upper limit of the performance grade is dependent on the G*/sin δ value; therefore, the relationship was also considered in terms of high end failure temperature.The rubber coefficient for G*/sin δ (R_cg) was identified as an important parameter in the estimation of G*/sin δ in addition to the CRM. This term is a quantitative representation of the increase typically witnessed in G*/sin δ values with the addition of CRM. Ambient ground CRM exhibited higher R_cg values than cryogenically ground particles. Additionally, 95% confidence intervals were generated for the predictive model, thus providing a range of accuracy for the model. The resulting confidence intervals were approximately ±1300 Pa, these confidence intervals were seen to capture 92.6% of the 462 data points used. Findings from this research suggest that the differences between cryogenic and ambient CRM binder are accurately described using the R_cg, furthermore binder properties may be predicted using an empirical equation.     

钢筋混凝土膜单元的简化设计和剪切强度计算

提出了在面内剪应力与正应力联合作用下膜单元破坏模式识别、设计和强度计算的简化方法。该SMCS(合成应力简化模型)的非迭代方法以修正受压理论(MCFT)的简化结果为基础。除了钢筋的作用大于"平衡"钢筋用量而被忽略,以及最大剪应力不超过断裂强度以外,这种简化使得方程与塑性理论方程相类似,断裂强度从普通和高强混凝土(100兆帕)膜试验数据中得到。以平面内剪应力和正应力为条件,对84个钢筋混凝土膜试验结果进行对比,试验剪切力强度与计算结果的比值平均为1·03,变异系数为11.3%。对于原始的修正受压理论,对应的值分别为0.99和11.1%。所提出方法的结果与ACI规范、简化的修正受压理论以及塑性理论也进行了比较,得到了满意的结果。49个试件中有45个破坏模式可以用所提出的合成应力简化模型准确计算,通过设计和承载力计算案例,对简化模型进行了验证。     

Ordering Cost Depletion in Inventory Policy with Imperfect Products and Backorder Rebate

This study presents an inventory model for imperfect products with depletion in ordering costs and constant lead time where the price discount in the backorder is permitted. The imperfect products are refused or modified or if they reached to the customer, returned and thus some extra costs are experienced. Lately some of the researchers explicitly present on the significant association between size of lot and quality imperfection. In practical situations, the unsatisfied demands increase the period of lead time and decrease the backorders. To control customers' problems and losses, the supplier provides a price discount in backorders during shortages. Also, an order’s policies may result in including some imperfect products in arrival lots. A discount on price may be offered by the supplier on the out-of-stock products to manage the backorder problems. The study aims to develop a model with imperfect products by permitting the price discount in backorders, and the cost of ordering is considered a decision variable. First, it is assumed that the demand for lead time is followed by a normal distribution and then stops it and assumed that the first two moments of demand for lead time are known. Further, the minimax distribution method is used to solve this model, and a separate algorithm is designed. In this study, two models are discussed with and without a normally distributed rate of demand. The current study verified with the help of some numerical examples over various model parameters.     

Determinants of metabolic acidosis among hemodialysis patients

Metabolic acidosis is frequently present, poorly controlled, and associated with adverse effects among hemodialysis patients. Potential determinants of metabolic acidosis include endogenous acid production, administration of alkali, neutralization of acid by buffers, dilution of serum bicarbonate by interdialytic fluid gain, and loss of bicarbonate in stool. Understanding the relative importance of these determinants may help guide efforts to manage metabolic acidosis. We used chart abstraction, patient interviews, and laboratory testing to assess variables related to acid production (protein breakdown), alkali administration (dialysis dose, missed treatments, dialysate bicarbonate concentration, oral bicarbonate supplements), acid buffering (phosphorus binders), dilution of bicarbonate (interdialytic weight gain), and loss of bicarbonate in stool (diarrhea) for 190 randomly selected patients from 44 hemodialysis facilities. We used multivariate analyses to determine which potential determinants were independently associated with predialysis serum bicarbonate levels. Of all patients, 30% had metabolic acidosis (serum bicarbonate level <22 mEq/L). On multivariate analysis, metabolic acidosis was more likely with increased protein nitrogen appearance (odds ratio [OR] 1.60 per 0.2 g/kg/day, p=0.001) and less likely with increased Kt/V (OR 0.61 per 0.20 increase in Kt/V, p<0.001) and with increased calcium carbonate use (OR 0.38 per 2 g/day, p=0.003). Key determinants of metabolic acidosis among hemodialysis patients are protein breakdown, dialysis dose, and specific phosphorus binders. Further work is needed to develop interventions to address these determinants.     

Toxicity and cellular uptake of gold nanorods in vascular endothelium and smooth muscles of isolated rat blood vessel: importance of surface modification

Gold nanorods (GNRs) have promising applications in drug delivery and cancer treatment and are generally administered via direct injection into the circulation. Thus it is necessary to evaluate their potential adverse effects on blood vessels. Herein, GNRs with various surface modifications are used to evaluate the toxicity and cellular uptake of GNRs into vascular endothelial and smooth muscle cells of isolated rat aortic rings. Surfactant-capped GNRs are synthesized and either coated with polyelectrolyte (PE) to prepare PE-GNRs, or modified with thiolated polyethylene glycol (PEG) to prepare PEG-GNRs. Using toxicity assays, small-vessel myography, fluorescence microscopy, and electron microscopy, it is shown that therapeutic concentrations of PE-GNRs but not PEG-GNRs are toxic to the vascular endothelium, which leads to an impaired relaxation function of aortic rings. However, no toxicity to smooth muscles is observed. Moreover, electron microscopy analysis confirms the cellular uptake of PE-GNRs but not PEG-GNRs into the endothelium of exposed aortic rings. The difference in toxicity and cellular uptake of PE-GNRs versus PEG-GNRs is explained and linked to free surfactant molecules and protein adsorption, respectively. The results indicate that toxicity and cellular uptake in the vascular endothelium in blood vessels are potential adverse effects of systemically administered GNR solutions, which can be prevented by appropriate surface functionalization.     

Effect of crumb rubber characteristics on crumb rubber modified (CRM) binder viscosity

Asphalt binder viscosity is of great importance during the production process of hot mix asphalt mixture as typically asphalt plants will store binders between 149 °C and 177 °C. SHRP guidelines state that asphalt binder viscosity must not exceed 3 Pa s. Therefore, given the documented increases in asphalt viscosity when modified with crumb rubber modifier (CRM) it is necessary to produce asphalt binder that fulfills the SHRP criteria while not exceeding plant mixing and storing requirements. This paper reports the results of an investigation of the importance of CRM properties on viscosity of CRM binder. Two binder sources were modified at four concentration levels using four different crumb rubber sources; the viscosities of the produced binders were evaluated by AASHTO T 316. Crumb rubber properties were evaluated by elemental analysis using a scanning electron microscope (SEM) and by determination of glass transition temperature (T_g) using a differential scanning calorimeter (DSC). In general, results indicate that processing procedure and tire type plays an important role in the determination of CRM binder viscosity.