Glycosylation in Indolent,Significant and Aggressive Prostate Cancer by Automated High-Throughput N-Glycan Profiling

The diagnosis and treatment of prostate cancer (PCa) is a major health-care concern worldwide. This cancer can manifest itself in many distinct forms and the transition from clinically indolent PCa to the more invasive aggressive form remains poorly understood. It is now universally accepted that glycan expression patterns change with the cellular modifications that accompany the onset of tumorigenesis. The aim of this study was to investigate if differential glycosylation patterns could distinguish between indolent, significant, and aggressive PCa. Whole serum N-glycan profiling was carried out on 117 prostate cancer patients’ serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins. We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease. These changes give us an insight into the disease pathogenesis and identify potential biomarkers for monitoring the PCa progression, however these need further confirmation studies.     

Improved modulation techniques for PWM-VSI drives

PWM-VSI based AC motor drives have two main problems. The inverter is nonlinear which causes instability problems in some specific working points of the AC machine and it emits acoustic noise due to the switching frequency. Nonlinearities like dead-time in the inverter, load dependent DC-link voltage ripple and the voltage drop across the switches are modeled and compensated by improved modulation techniques in order to obtain an almost ideal inverter. Different feedback and feedforward techniques are proposed. The acoustic noise is reduced by using a random modulation strategy. Measurements show a significant improvement by using feedforward and feedback techniques for linearizing the inverter. An improvement in reduction of the acoustic noise emission is also achieved by using random modulation. It is concluded that a combination of a random modulation strategy and feedforward/feedback techniques gives an almost ideal AC motor drive system     

Frontiers in research on cystic fibrosis: understanding its molecular and chemical basis and relationship to the pathogenesis of the disease

In recent years a new family of transport proteins called ABC transporters has emerged. One member of this novel family, called CFTR (cystic fibrosis transmembrane conductance regulator), has received special attention because of its association with the disease cystic fibrosis (CF). This is an inherited disorder affecting about 1 in 2000 Caucasians by impairing epithelial ion transport, particularly that of chloride. Death may occur in severe cases because of chronic lung infections, especially by Pseudomonas aeruginosa, which cause a slow decline in pulmonary function. The prospects of ameliorating the symptoms of CF and even curing the disease were greatly heightened in 1989 following the cloning of the CFTR gene and the discovery that the mutation (deltaF508), which causes most cases of CF, is localized within a putative ATP binding/ATP hydrolysis domain. The purpose of this introductory review in this minireview series is to summarize what we and others have learned during the past eight years about the structure and function of the first nucleotide binding domain (NBF1 or NBD1) of the CFTR protein and the effect thereon of disease-causing mutations. The relationship of these new findings to the pathogenesis of CF is also discussed.     

Cardiac risk in surgery. A review and guidelines for risk evaluation and reduction of cardiac risk in general surgery

We report herein the phenotypic and functional analysis of human bone marrow and thymus derived early T cells. Commitment to T cell lineage is acquired during CD7 antigen expression by CD34+ precursors in human bone marrow and before thymus colonization. Early thymocytes show similar phenotypic characteristics as bone marrow T cells. They rapidly acquire CD4 before the dual expression of CD4 and CD8. Their expansion and differentiation is regulated by two major factors: thymic stroma and cytokines produced by these stroma cells or by thymocytes themselves. Among cytokines, IL1 and sCD23 produced by thymic epithelial cells support in vitro early T cell development.     

Excited-state-absorption cross sections and amplifier modeling inthe 1300-nm region for Nd-doped glasses

The performance of Nd³⁺-doped fibre amplifiers is limited by strong excited-state absorption (ESA) of the signal, and, even for fluorozirconate glasses, ESA prevents the important region below 1320 nm from being used. To quantify this limitation and explore alternative host materials, ESA and stimulated-emission cross sections have been measured for a representative group of glass compositions. These parameters have been used in an accurate fiber-amplifier model to provide the first quantitative comparisons of performance for Nd³⁺ -doped glasses in the 1300-nm band as a function of host     

Room electromagnetics

In analogy with the established discipline of room acoustics, various aspects of diffuse wideband microwave propagation in a room are treated. It is shown that an equivalent to Sabine's equation for reverberation time in a room is valid for the completely diffused field, depending only on the volume, the surface area, and an effective absorption coefficient. An exponential decay of the power as a function of the delay is a consequence of the assumptions. Furthermore, the concept of a reverberation distance is also valid. This is the distance from a transmitting antenna where the received diffuse, randomly scattered power equals the direct line-of-sight received power, such that the diffuse power dominates for distances larger than the reverberation distance. A number of measurements in a large room support the theory with an effective absorption coefficient of 0.5. The power delay profiles around the room from a transmitter in the ceiling vary only in the first arriving part of the impulse, whereas the tail, being dominated by the diffuse field, has the same power level for a given delay and the same decay rate all over the room. It is also a consequence of the theory that the diffuse fields incident on an antenna are uniformly distributed in angle.     

Buckling behaviour of imperfect spherical shells subjected to different load conditions

The paper deals with an analysis of the bifurcation and initial post-buckling behaviour of highly imperfection-sensitive large spherical .shells, such as cargo tanks for ship transportation of liquefied natural gas and large spherical containment shells for nuclear power plants. The numerical analysis procedure has sufficient generality to treat shells of revolution with arbitrary curved generators and with arbitrary variation of the thickness. The shells can be subjected to non-axisymmetric time-varying loadings. The purpose of the paper is to present simple procedures for scantling selections in the initial design phase and to propose an analysis procedure for verification of the final design of optimized thin-walled spherical shells.     

Does genetic variance for cognitive abilities account for genetic variance in educational achievement and occupational status? A study of twins reared apart and twins reared together

Studies of brothers and twins have shown that about 50 per cent of the variance in educational achievement and 40 per cent of the variance in occupational status reflects between-family variance. About half of the between-family variance for educational achievement and even more for occupational status is due to genetic effects and the remainder is due to sharing the same environment. With data on 35 pairs of male twins reared apart and 56 pairs reared together we investigated the extent to which genetic variance in SES can be attributed to genetic variance for cognitive abilities. For both educational achievement and occupational status there was significant genetic variance both in common with and independent of genetic variance for cognitive abilities. Thus, there are genetic effects contributing to familial similarity for SES that are not the same as those of importance for cognitive abilities. Candidate traits that may account for this remaining genetic variance in SES are personality, interests, or talents not represented in standard cognitive tests.