Chrysin,Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages

Expression level of Tumor Necrosis Factor—related apoptosis—inducing ligand (TRAIL) receptors is one of the most important factors of TRAIL-mediated apoptosis in cancer cells. We here report for the first time data concerning TRAIL-R1 and TRAIL-R2 receptor expression on RAW264.7 macrophages. Three substances belonging to flavones: chrysin, apigenin and acacetin which differ from their substituents at the 4'' position in the phenyl ring were used in assays because of the variety of biological activities (e.g., anticancer activity) of the polyphenol compounds. The expression of TRAIL-R1 and TRAIL-R2 death receptors on non-stimulated and LPS (lipopolysaccharide)-stimulated macrophages was determined using flow cytometry. We demonstrate that RAW264.7 macrophages exhibit TRAIL-R1 surface expression and that the tested compounds: chrysin, apigenin and acacetin can inhibit TRAIL-R1 death receptor expression level on macrophages.     

A Simple Pneumatic Loading System Controlling Stress and Strain Rates for One-Dimensional Compression of Clay

A simple but automated pneumatic loading system that can control the stress and strain rates for one-dimensional (1D) compression of clay was developed. The rate-dependency of stress-strain behaviour due to the viscous property of clay was investigated by 1D compression tests on standard-size specimens by various loading methods: 1) Standard Consolidation Tests (SCTs), stepwise increasing the axial stress two times every one day; 2) ordinary Constant-Rate-of-Strain (CRS) tests at different strain rates; 3) special CRS tests including unloading and reloading cycles with different stress amplitudes at strain rates of which the absolute value was either kept constant throughout respective tests or changed at the start of reloading; and 4) special CRS tests including a number of sustained loading (SL) during otherwise primary loading or unloading or reloading at constant strain rate. Sufficiently low strain rates were employed to ensure essentially fully drained condition. The followings were found. Despite that the newly developed pneumatic loading system is rather simple, 1D compression tests following such various loading histories as above can be performed on four types of clay rather accurately. The stress-strain behaviour of clay is significantly rate-dependent, exhibiting significant creep strains at SL stages. The creep strain rate is significantly different whether SL starts during otherwise primary loading or unloading or reloading, controlled by the magnitude and sign of the initial strain rate at the start of SL. The whole observed trends of rate-dependent stress-strain behaviour can be qualitatively explained by the non-linear three-component elasto-viscoplastic model extended to cyclic loading conditions.     

Viscous Behaviour of Unbound Granular Materials in Direct Shear

The viscous properties of a variety of poorly graded unbound granular materials were investigated by direct shear tests on 12 cm-cubic specimens. A number of natural sands having different particle shapes and sizes as well as uniform glass beads having different particle sizes were used. The viscous properties were evaluated by changing the shear displacement rate many times during otherwise monotonic loading (ML) at constant shear displacement rate and normal pressure. Creep loadings were performed in two tests. Different types of viscous properties, which are affected by the particle shape but essentially independent of the particle size, are reported. The viscosity type varies as the shear displacement increases from the pre-peak regime towards the residual state. A new viscosity type, called “Positive & Negative”, was found with relatively round granular materials in the pre-peak regime and with relatively angular granular materials in the post-peak softening regime and at the residual state. Peculiar “rate-independent unstable behaviour” is observed with round natural sands and glass beads in the post-peak regime, which is more significant and frequent with glass beads. Controlled by the particle size, this behaviour is caused by the so-called stick/slip phenomenon. The viscous properties observed in the DS tests are quantified by the rate-sensitivity coefficient defined in terms of the shear and normal stresses, which are then converted to those defined in terms of the major and minor principal stresses, β₁₃. These β₁₃ values are consistent with those directly obtained by the triaxial and plane strain compression tests. The effects of particle size on the β₁₃ value are negligible and the β₁₃ value tends to decrease as the particle shape becomes more round.     

Nonlinear load–strain modeling of polypropylene geogrids during constant rate‐of‐strain loading

This article describes a rate‐dependent hyperbolic model that was developed to predict the tensile load–strain behavior of a polypropylene geogrid reinforcement material under monotonic and stepped constant rate‐of‐strain testing. A more general three‐component model previously reported in the literature was also used in the current study but with some modifications to compute model parameters. Details of the trial and error procedure to select three‐component model parameters, not previously reported in the literature, are explained. Both models gave similar good agreement between measured and predicted constant rate‐of‐strain tests. The accuracy of the three‐component model to simulate stepped constant rate‐of‐strain tests was judged to be better, but for practical purposes, the simpler hyperbolic model was judged to be satisfactory. An advantage of the hyperbolic model is that the model parameters are easy to determine, only monotonic constant rate‐of‐strain tests are required, and numerical implementation is simple. However, the hyperbolic model is restricted to monotonic or stepped constant rate‐of‐strain load paths. An advantage of the more complicated three‐component model is that it has been demonstrated in previous studies to be more general and thus can be used for other load paths and other polymeric reinforcement material types that do not have characteristic hyperbolic load–strain behavior. POLYM. ENG. SCI., 55:1617–1627, 2015. © 2014 Society of Plastics Engineers     

Strain rate effects on sand and its quantitative analysis

Strain rate effects on the stress—strain behavior of sand were investigated by performing special plane strain and triaxial compression tests on saturated and air-dried sand specimens. In these tests, the loading strain rate was changed many times by a factor of up to 1 000 during otherwise monotonous loading at a constant axial strain rate. Test results show that the stress jump upon a stepwise change in the strain rate decays with an increase in the irreversible strain when monotonous loading continues at the changed strain rate and the amount of stress jump is essentially proportional to the instantaneous stress. Based on the amount of these stress jumps, a parameter β called the rate-sensitivity coefficient is introduced to represent the quantity of the observed viscous properties of sand, which equals 0.021 3 and 0.024 2 respectively for Hostun and Toyoura sands. Further analyses on the results indicate that the effect of the presence of pore water is deemed to be negligible with sand and the β value is rather independent of loading method, wet condition and confining pressure. Foundation item: Project(50679056) supported by the National Natural Science Foundation of China; Project(06-0378) supported by Program for New Century Excellent Talents in University; Project(05SG25) supported by the “Dawn” Program of Shanghai Education Commission, China; Project(B308) supported by the Shanghai Leading Academic Discipline, China     

Modelling of Ageing Effects on the Elasto-Viscoplastic Behaviour of Geomaterial

The time effects on the stress-strain behaviour of geomaterial consist of effects of loading rate and ageing. The positive ageing effects are analysed based on the results from drained triaxial compression (TC) tests on cement-mixed kaolin and well-graded gravelly soil and incorporated into a non-linear three-component model that can simulate the elasto-viscoplastic behaviour of geomaterials. The inviscid yielding is controlled by the inviscid yield stress that develops basically by irreversible straining and time elapsing following, respectively, a basic inviscid strain-hardening function and an ageing function. The inviscid yield stress may develop additionally by positive interaction between ageing and inviscid yielding following an interaction function, which expresses an additional strength gain by longer ageing at higher shear stress levels. Positive interaction effects are damaged by subsequent irreversible straining following a damage function. These functions are formulated based on experimental results. Illustrative model simulations are presented to describe the structure of the proposed model. The model is validated by simulating drained TC tests exhibiting significant effects of loading rate and ageing.     

FEM simulation of viscous properties for granular materials considering the loading rate effect

The deformation and strength characteristics of sandy soils as a kind of granular materials are very complex. The experimental results show that when the strain rate suddenly changes in monotonic loading (ML) case, the stress–strain curve of sandy soils changes sharply and then gradually converges into the original inferred one that would be obtained by continuous ML at constant strain rate after having exhibited clear yielding. Similar behaviors are also observed when ML is restarted at a constant strain rate following a creep loading or stress relaxation stage. An elasto-viscoplastic constitutive model for granular materials is developed, which consists of three components. One of the most important features of the model is that it can take into account the effects of loading rate due to viscous properties on the stress–strain behavior. The stress ratio-axial strain–time relations from four drained plain strain compression (PSC) tests on the saturated Toyoura sand are successfully simulated by the finite element method (FEM) code incorporating the proposed constitutive model. It is shown that the FEM code can simulate the viscous behaviors of sand accurately under arbitrary loading history.     

Residual Deformation of Geosynthetic-Reinforced Sand in Plane Strain Compression Affected by Viscous Properties of Geosynthetic Reinforcement

A series of plane strain compression (PSC) tests were performed on large sand specimens unreinforced or reinforced with prototype geosynthetic reinforcements, either of two geogrid types and one geocomposite type. Local tensile strains in the reinforcement were measured by using two types of strain gauges. Sustained loading (SL) under fixed boundary stress conditions and cyclic loading (CL) tests were performed during otherwise monotonic loading at a constant strain rate to evaluate the development of creep deformation by SL and residual deformation by CL of geosynthetic-reinforced sand and also residual strains in the reinforcement by these loading histories. It is shown that the creep deformation of geosynthetic-reinforced sand develops due to the viscous properties of both sand and geosynthetic reinforcement, while the residual deformation of geosynthetic-reinforced sand during CL (defined at the peak stress state during CL) consists of two components: i) the one by the viscous properties of sand and reinforcement; and ii) the other by rate-independent cyclic loading effects with sand. The development of residual deformation of geosynthetic-reinforced sand by SL and CL histories had no negative effects on the subsequent stress-strain behaviour and the compressive strength was maintained as the original value or even became larger by such SL and CL histories. The local tensile strains in the geosynthetic reinforcement arranged in the sand specimen subjected to SL decreased noticeably with time, due mainly to lateral compressive creep strains in sand during SL of geosynthetic-reinforced sand. This result indicates that, with geosynthetic-reinforced soil structures designed to have a sufficiently high safety factor under static loading conditions because of seismic design, it is overly conservative to assume that the tensile load in the geosynthetic reinforcement is maintained constant for long life time. Moreover, during CL of geosynthetic-reinforced sand, the residual tensile strains in the geosynthetic reinforcement did not increase like global strains in the geosynthetic-reinforced sand that increased significantly during CL. These different trends of behaviour were also due to the creep compressive strains in the lateral direction of sand that developed during CL of geosynthetic-reinforced sand.     

Effects of loading rate on viscoplastic properties of polymer geosynthetics and its constitutive modeling

On the basis of the special tensile test results under various loading histories, the rate‐dependent behaviors of three polymer geosynthetics due to their viscous properties have been investigated. All the investigated polymer geosynthetics show significant loading rate effects, creep deformation, and stress relaxation. Except for the polyester geogrid showing the combined viscosity, all the investigated polymer geosynthetics exhibit the isotach viscosity. An elasto‐viscoplastic constitutive model described in a nonlinear three‐component model framework is developed to simulate the rate‐dependent behaviors of polymer geosynthetics. The developed constitutive model is verified by comparing its simulated results with the experimental data of polymer geosynthetics presented in this study and those available from the literature. The comparison indicates that the developed model can reasonably interpret the rate‐dependent behaviors of polymer geosynthetics under arbitrary loading histories, including the step‐changed strain rate loading, creep, and stress relaxation applied during otherwise monotonic loading (ML). POLYM. ENG. SCI., 2010. © 2009 Society of Plastics Engineers