DETECTION OF BACILLUS CEREUS DIARRHEAGENIC TOXIN USING A RAT LIGATED INTESTINAL LOOP ASSAY

A study was performed to determine if the rat ligated intestinal loop assay could detect Bacillus cereus diarrheagenic toxin. The results obtained indicated that this assay system could be used for such a purpose but it was not as sensitive as the young rabbit ligated ileal loop assay. When rats were used, concentrating the cell free culture filtrate of B. cereus 30- to 40-fold was necessary to detect diarrheagenic toxin activity with the intestinal loop assay.     

SENSITIVITY OF LISTERIA MONOCYTOGENES TO SPICES AT TWO TEMPERATURES

Thirteen spices were screened for growth inhibition effect against Listeria monocytogenes at 24°C using a concentration gradient plate method. Cloves, and oregano were the two most inhibitory spices with MIC values ranging from 0.5 to 0.7% (W/V). Inhibition was also detected with sage and rosemary (MIC of 0.7 to 1.0%) as well as nutmeg (MIC of 1.1–1.4%). Black pepper, chili, cinnamon, garlic, mustard, paprika, parsley and red pepper at a concentration up to 3.0% did not inhibit the growth of the organisms. The effect of cloves, oregano and sage on the growth and survival of L. monocytogenes Scott A was further tested in tryptic soy broth at both 4 and 24°C. At a concentration of 0.5 or 1.0%, cloves were bactericidal and oregano was bacteriostatic to this organism at both incubation temperatures. Sage at these two concentrations was bactericidal at 4°C and bacteriostatic at 24°C. When tested in sterile meat slurry, a 1.0% level of either cloves or oregano had little effect on the growth of L. monocytogenes Scott A at 4 or 24°C.     

基于 FTA-BN模型的城市燃气管道失效风险分析

为了找出导致城市燃气管道失效事故的原因,以城市燃气管道失效事故为研究对象,采用故障树方法和贝叶斯网络模型对其进行分析。通过实例分析,找出了导致某市城市燃气管道失效事故概率较大的因素分别为：阴极保护失效、外腐蚀、内腐蚀、外力影响、施工缺陷等。结果表明,基于FT A BN模型的城市燃气管道失效风险分析方法注重基事件的多态性和事件间逻辑关系合理性,能推算出更准确的基事件概率分布,同时可以找出导致事故发生的最有可能途径,为城市燃气管道失效事故预防、风险控制、安全管理提供较为合理性建议。     

基于关联度流量预测的加权局域模型

传统的混沌局域预测模型仅考虑相空间中邻近相点到预测中心的空间距离对预测中心动力学行为演化的影响,所以在嵌入维数比较高的时候,用来预测混沌时间序列时效果往往不是很好.本文提出了一种基于关联度的网络流量预测的加权零阶局域模型,该模型同时综合考虑到了空间距离和邻近相点与预测中心的关联程度这两个因素对预测效果的影响,因而在很大程度上克服了传统混沌局域模型的不足.通过模拟实验将该模型用于网络流量预测,模拟结果表明该模型的预测精度比传统的模型要好.     

基于Sobel算子的图像边缘检测及其实现

Sobel算子是图像边缘检测中常用的方法之一,利用像素的左、右、上、下邻域的灰度加权算法,根据在边缘点处达到极值这一原理进行边缘检测。该方法不但产生较好的检测效果,而且对噪声具有平滑作用,可以提供较为精确的边缘方向信息。本文提供了利用Sobel算子实现灰度图像边缘检测的C 源程序。     

引发电控模块短路之PCB孔口发黑原因研究

PCB在使用过程中在经常会出现短路故障,对相关故障电子模块进行分析时发现模块PCB较多孔口存在发黑物质,严重者出现黑色物质连孔,通过测试确认黑色物质具有导电功能。本文通过理论分析及模拟实验论证,确认了黑色物质是引发电子模块故障的根源,同时明确了其产生机理,并提出了针对性的预防措施。     

Lightning Observation in 3D Using a Multiple LF Sensor Network and Comparison with Radar Reflectivity

A three‐dimensional (3D) lightning mapper consisting of multiple low‐frequency (LF) sensors has been developed to monitor thunderstorms and to estimate their charge structure. We have been conducting an observation campaign using the 3D lightning mapper in Kansai, Japan, since October 2012. In this study, we determine 3D source locations of intracloud and cloud‐to‐ground flashes and compare them with the radar reflectivity estimated by phased‐array radar (PAR) with high temporal and spatial resolution. Most LF sources are located in high radar reflectivity regions, confirming that the 3D LF lightning mapper monitors thunderstorms. A lightning flash with heavy branches is clearly imaged. Based on the bidirectional leader theory that negative and positive ends of a lightning leader develop simultaneously, we estimate the positive and negative charge regions in a thundercloud from the LF source locations. The radar reflectivity estimated by PAR supports the charge structure estimated by LF source locations.     

PCB内埋入空气腔体制作工艺研究

通讯技术的高速化要求PCB具有传输损失(α)小、传输延迟时间(Tpd)短、信号传输的失真小的特性,这就要求PCB使用的基板材料有优秀的介电特性,以及对特性阻抗(Zo)的高精度控制。空气的Dk(1.00053)仅次于真空下的Dk。因此如果能在背板的阻抗线路位置制作成空气腔体,实现信号的高质量高速传递。文章采用低流胶粘结材料/掩膜材料在PCB内埋入多个、多层空气腔体,对比了不同空腔材料制作空气腔体的空腔形态、耐热性能,研究了空腔形态的影响因素。     

(S/R)-2-(2-(5-氟-2,4-二氧-3,4-二氢嘧啶-1(2H)-基)乙酰氨基)-3-(4-羟苯基)丙酸的合成及抗肿瘤活性

以1-乙基-3-(3-二甲胺丙基)碳二亚胺盐酸盐(EDCI)/1-羟基苯并三氮唑(HOBt)为缩合剂,5-氟尿嘧啶-1-基乙酸为中间体,分别与L-酪氨酸甲酯和D-酪氨酸乙酯通过液相偶联合成了(S)-2-(2-(5-氟-2,4-二氧-3,4-二氢嘧啶-1(2H)-基)乙酰氨基)-3-(4-羟苯基)丙酸甲酯和(R)-2-(2-(5-氟-2,4-二氧-3,4-二氢嘧啶-1(2H)-基)乙酰氨基)-3-(4-羟苯基)丙酸乙酯,水解后得到相应的酸对映体。所合成的化合物结构经1H NMR1、3C NMRI、R、MS及比旋光度等测试得以确证。四种化合物的体外抗肿瘤活性测试结果,说明该化合物的抗肿瘤具有一定的选择性,且R构型对抗肿瘤也起到了一定的作用。     

机械控深盲孔技术开发与研究

文章对影响机械控深盲孔的因素（层压厚度公差、机械钻机的深度控制能力等）进行研究，同时对机械控深盲孔PCB的制作流程进行平行试验研究；得出机械控深盲孔的制作精度，机械控深盲孔PCB的制作工艺参数，机械控深盲孔应用的潜在失效模式，并提出一些改善建议。