Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene

Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falciparum, Plasmodium yoelii, and Plasmodium gallinaceum are vastly divergent yet share a similar codon usage and total A/T content of approximately 82%. This is in contrast to other primate-specific species including P. vivax which have an A/T content of approximately 67%. To assess the effects of this evolutionary divergence on the conservation of gene content, organization, and codon usage in the mitochondrial DNA (mtDNA) of malaria parasites, we have cloned and sequenced the mitochondrial genome of Plasmodium vivax, and compared it with the mtDNAs of P. falciparum, P. yoelii, and P. gallinaceum. The P. vivax mitochondrial genome was found to be 5990 base pairs in length, and displayed a gene organization identical to that of P. falciparum, P. yoelii, and P. gallinaceum. Furthermore, there was a remarkable 90% conservation of sequence identity between the mitochondrial genomes of all four species. As an example of intra-species conservation, comparison of mtDNAs from two independently cloned P. falciparum isolates, Malay Camp and C10, revealed only a single nucleotide substitution. A/T content of the P. vivax mitochondrial genome was found to be identical to other species of Plasmodium, hence, we have postulated that the mitochondrial genomes of malaria parasites were refractory to the evolutionary shifts in nucleotide content seen among the nuclear genomes of malaria parasites. Among different Plasmodium species, the second position of mitochondrial codons were found to be the least prone to substitutions and displayed a significant bias in pyrimidines. These aspects of mitochondrial codon usage were distinct from the nuclear genome and may reflect functional aspects of decoding by the mitochondrial translational system.     

Approximability of Clausal Constraints

We study a family of problems, called Maximum Solution (Max Sol), where the objective is to maximise a linear goal function over the feasible integer assignments to a set of variables subject to a set of constraints. When the domain is Boolean (i.e. restricted to {0,1}), the maximum solution problem is identical to the well-studied Max Ones problem, and the complexity and approximability is completely understood for all restrictions on the underlying constraints. We continue this line of research by considering the Max Sol problem for relations defined by regular signed logic over finite subsets of the natural numbers; the complexity of the corresponding decision problem has recently been classified by Creignou et al. (Theory Comput. Syst. 42(2):239–255, 2008). We give sufficient conditions for when such problems are polynomial-time solvable and we prove that they are APX-hard otherwise. Similar dichotomies are also obtained for variants of the Max Sol problem.     

Induction and Synchronous Motors Compared for System Stability

A comparison is made between synchronous motors and induction motors with regard to industrial power system stability. Representation of motor loads in stability studies is reviewed. The specific system studied is an infinite bus supplying a combination induction and synchronous motor load through a transmission line and transformer. The system is subjected to a fault at the transformer and critical values of fault clearing time are computer calculated for ratios of synchronous motor load to induction motor load from zero to unity. The effect of variation of motor parameters on clearing times is discussed.     

Schrifttumsberichte

Ohne Zusammenfassung     

Schrifttumsberichte

Ohne Zusammenfassung     

Energy cost and error performance of range-aware, anchor-free localization algorithms

Many applications that use sensor data from a wireless sensor network (WSN) require corresponding node position information as well. Therefore, it is not surprising that a common figure of merit for localization algorithms is the accuracy of the position estimate produced. Similarly, the amount of communication required by a localization algorithm is often of paramount interest as well since it is common knowledge that communication expends the most energy in a WSN. However, localization algorithms seldom characterize their communication cost. Furthermore, when they do it is often merely qualitative and is typically described as “expensive”. For two types of range-aware, anchor-free localization algorithms we found the opposite to be true. Rather than being expensive, the communication costs were quite modest. So much so that we maintain range-aware, anchor-free localization algorithms should be chosen on the basis of the accuracy required by the intended application independent of the communication cost.In this paper, we examine the effect of node degree, node distribution, range error and network size on distance error and communication cost for both incremental and concurrent versions of range-aware, anchor-free algorithms. The concurrent algorithm is twice as accurate as the incremental, but less efficient. Furthermore, node degree influences the energy cost of the algorithms the most, but neither algorithm uses more than a surprisingly small 0.8% of a 560 mA h battery. This result indicates less energy efficient localization algorithms can be tolerated, especially if they provide better accuracy. Furthermore, if energy does need to be conserved, there is not much savings available within the localization algorithm and savings must be found in other areas such as the MAC protocol or routing algorithm.     

Convex optimization of measurement allocation for magnetic tracking systems

Magnetic tracking is a popular technique that exploits static and low-frequency magnetic fields for positioning of quasi-stationary objects. One important system design aspect, which substantially influences the performance of the tracking system, is how to collect as much information as possible with a given number of measurements. In this work, we optimize the allocation of measurements given a large number of possible measurements of a generic magnetic tracking system that exploits time-division multiplexing. We exploit performance metrics based on the Fisher information matrix. In particular, the performance metrics measure worst-case or average performance in a measurement domain, i.e. the domain where the tracking is to be performed. An optimization problem with integer variables is formulated. By relaxing the constraint that the variables should be integer, a convex optimization problem is obtained. The two performance metrics are compared for several realistic measurement scenarios with planar transmitter constellations. The results show that the worst performance is obtained in the most distant parts of the measurement domain. Furthermore, measurement allocations optimized for worst-case performance require measurements in a larger area than measurement allocations optimized for average performance.     

Ion Electrodiffusion Governs Silk Electrogelation

Silk electrogelation involves the transition of an aqueous silk fibroin solution to a gel state (E-gel) in the presence of an electric current. The process is based on local pH changes as a result of water electrolysis - generating H(+) and OH(-) ions at the (+) and (-) electrodes, respectively. Silk fibroin has a pI=4.2 and when local pH相似文献   

Spektroskopische Untersuchungen an organischen Carbonylverbindungen. VI. NMR-Studien an substituierten β-Diketonen und ihren vinylogen Derivaten

Spectroscopic Investigations on Organic Carbonyl Compounds. VI. N.M.R.-Studies on Substituted β;-Diketones and their Vinyloge Derivatives . ¹³C- and ¹H-n.m.r.-data of substituted β-diketones and their vinyloge derivatives are presented. Most of the compounds exist chiefly in the enol-form. Only in the case of 4-dimethyl-aminodibenzoylmethane a keto-enol-equilibrium is registered. The influence of the substituent A and the chainlength on the position of the enol proton is discussed.