Estimation of apparent rate coefficients for radionuclides interacting with marine sediments from Novaya Zemlya

To assess the impact of radionuclides entering the marine environment from dumped nuclear waste, information on the physico-chemical forms of radionuclides and their mobility in seawater-sediment systems is essential. Due to interactions with sediment components, sediments may act as a sink, reducing the mobility of radionuclides in seawater. Due to remobilisation, however, contaminated sediments may also act as a potential source of radionuclides to the water phase. In the present work, time-dependent interactions of low molecular mass (LMM, i.e. species < 10 kDa) radionuclides with sediments from the Stepovogo Fjord, Novaya Zemlya and their influence on the distribution coefficients (Kd values) have been studied in tracer experiments using 109Cd2+ and 60Co2+ as gamma tracers. Sorption of the LMM tracers occurred rapidly and the estimated equilibrium Kd(eq)-values for 109Cd and 60Co were 500 and 20000 ml/g, respectively. Remobilisation of 109Cd and 60Co from contaminated sediment fractions as a function of contact time was studied using sequential extraction procedures. Due to redistribution, the reversibly bound fraction of the gamma tracers decreased with time, while the irreversibly (or slowly reversibly) associated fraction of the gamma tracers increased. Two different three-compartment models, one consecutive and one parallel, were applied to describe the time-dependent interaction of the LMM tracers with operationally defined reversible and irreversible (or slowly reversible) sediment fractions. The interactions between these fractions were described using first order differential equations. By fitting the models to the experimental data, apparent rate constants were obtained using numerical optimisation software. The model optimisations showed that the interactions of LMM 60Co were well described by the consecutive model, while the parallel model was more suitable to describe the interactions of LMM 109Cd with the sediments, when the squared sum of residuals were compared. The rate of sorption of the irreversibly (or slowly reversibly) associated fraction was greater than the rate of desorption of the reversibly bound fractions (i.e. k3 > k2) for both radionuclides. Thus, the Novaya Zemlya sediment are supposed to act as a sink for the radionuclides under oxic conditions, and transport to the water phase should mainly be attributed to resuspended particles.     

Degradation Products of Tryptophan in Cell Culture Media: Contribution to Color and Toxicity

Biomanufacturing processes may be optimized by storing cell culture media at room temperature, but this is currently limited by their instability and change in color upon long-term storage. This study demonstrates that one of the critical contributing factors toward media browning is tryptophan. LC-MS technology was utilized to identify tryptophan degradation products, which are likely formed primarily from oxidation reactions. Several of the identified compounds were shown to contribute significantly to color in solutions but also to exhibit toxicity against CHO cells. A cell-culture-compatible antioxidant, a-ketoglutaric acid, was found to be an efficient cell culture media additive for stabilizing components against degradation, inhibiting the browning of media formulations, and decreasing ammonia production, thus providing a viable method for developing room-temperature stable cell culture media.     

Short-term temporal variations in speciation of Pb, Cu, Zn and Sb in a shooting range runoff stream

This study was designed to explore the changes in physico-chemical forms of Pb, Cu, Zn and Sb in a stream draining a contaminated shooting range, located at Steinsjøen in the South-Eastern part of Norway, during a period of 21 days. To obtain information on the element species distribution, an interphased size and charge fractionation system was applied, where membrane filtration (0.45 µm) and ultrafiltration using hollow fibre (nominal cut off 10 kDa) were performed prior to charge fractionation using chromatography (cationic and anionic exchange resins). The results show that Pb mainly was present as particulate and colloidal high molecular mass (HMM) species, Cu as colloidal (HMM) and low molecular mass (LMM) species, while Sb and Zn were mainly present as LMM species. The total element concentrations of Pb, Cu, Zn and Sb were positively correlated to water flow and dissolved organic carbon (DOC), suggesting these are important factors in controlling the run-off of the investigated elements in this catchment. During episodes of higher water flow, the increase in element concentration was mainly in the colloidal fraction. Partial redundancy analysis (pRDA) revealed that variations in pH, HMM organic carbon (HMM OC) and LMM organic carbon (LMM OC) explained 47% of the variation in size distribution of the elements, while variations in precipitation and water flow explained 48% of the variation in the charge distribution of the elements. The variation in concentrations during the period varied by a factor of 4, also stressing the importance of frequent sampling opposed to spot sampling in environmental surveys and risk assessments.     

Visual search for singleton feature targets across dimensions: Stimulus- and expectancy-driven effects in dimensional weighting.

Four pop-out search experiments investigated whether dimension-based visual attention is top-down modulable. Observers searched for singleton feature targets defined, variably across trials, by a color or an orientation difference to nontargets. Observers were precued to the most probable target-defining dimension (e.g., by the word color) or feature (red) on a given trial. Results revealed expedited reaction times (RTs) for valid-dimension targets relative to neutral-cue conditions, and slowed RTs for invalid-dimension targets. Cue information as to precise target feature yielded some extra effect only for color targets. The dimensional cuing significantly reduced, but did not abolish, the dimension-specific influence of the previous target on detection of the current target (same-dimension RT     

ICT and Integrated Care: Some Dilemmas of Standardising Inter-Organisational Communication

There is a growing interest in the issues of how to organise healthcare work along individual patient cases rather than along the demarcation lines of healthcare organisations. Health information systems, such as electronic patient records, are seen as important change agents, since they are asserted to help the coordination of care across organisations through fast and accurate exchange of clinical data. The paper explores how a semi-standardised discharge letter is employed to communicate about the patient between two organisational settings, the hospital and the general practitioner. It is shown that the discharge letter plays a double role as informational tool and accounting device. And it is argued that further standardisation of the discharge letter content – in order to facilitate electronic exchange – is likely to strengthen the letters role as a tool for organisational accountability and weaken it as a clinical tool. The paper concludes that this finding adds to the theoretical understanding of how computers support cooperative work, and that understanding how healthcare professionals present themselves as accountable and trustworthy should be of major concern when designing healthcare ICTs.     

Gill reactivity of aluminium-species following liming

In acidified aluminium (Al) rich freshwater positively charged Al-species (Al(i)) are the key toxic components due to the accumulation in fish gills. As a countermeasure, liming is used to increase the pH and reduce the concentration of Al(i)-species; in particular low molecular mass (LMM) Al(i)-species by hydrolyses. However, very toxic high molecular mass (HMM) Al polymers can form in the unstable mixing zone immediately after liming. In the present work gill reactivity of LMM and HMM Al-species was studied under controlled conditions in eight channel-tank mixing zone systems in the field where Atlantic salmon (Salmo salar L.) kept in cages were exposed to defined mixing zone water. Mixing zones were created by continually liming acid river water (pH 5.0-5.7) high in LMM Al(i) to pH 6.0 and 6.4, respectively. Transformation processes affecting the Al-speciation as a function of time after liming were documented by in situ hollow fibre ultrafiltration interfaced with ion chromatography, while the Al accumulation in fish gills was used as bioindicator. For fish exposed to mixing zone water immediately after liming (1 min) the Al accumulation in gills (mug Al g(-1)) was higher (factor of 2) than for fish exposed to acid water prior to liming, due to the formation of gill reactive HMM Al(i)-species. The Al accumulation in gills followed a first order kinetic expression reaching steady-state conditions after 24-h exposures. The deposition rate of Al in gills (mug Al g(-1) h(-1)) correlated with the water concentrations of HMM Al(i) (R(2)=0.80) in the mixing zones, and for LMM Al(i) in the acid water (R(2)=0.92). Due to the transient nature of HMM Al(i) the deposition rate of Al decreased from the point of liming with a factor of 10 downstream the channel-tank system (i.e. 100 min after liming). The concentration of gill accumulated Al was higher (factor of 3) immediately after high level liming (pH 6.4) than following low level liming (pH 6.0). However, high level liming was more efficient in detoxifying Al in downstream waters. Furthermore, the bioavailability of a given LMM Al(i) concentration as well as bioreactivity following liming was dependent on the TOC and silicon concentration in the acid water. Increased concentration of TOC (1.5 to 4.3 mg l(-1)) and silicon (0.3 to 1.0 mg l(-1)) reduced the gill reactivity of Al(i) by approximately 50%.     

Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress

Splicing is an important RNA processing step. Genetic variations can alter the splicing process and thereby contribute to the development of various diseases. Alterations of the splicing pattern can be examined by gene expression analyses, by computational tools for predicting the effects of genetic variants on splicing, and by splicing reporter minigene assays for studying alternative splicing events under defined conditions. The minigene assay is based on transient transfection of cells with a vector containing a genomic region of interest cloned between two constitutive exons. Cloning can be accomplished by the use of restriction enzymes or by site-specific recombination using Gateway cloning. The vectors pDESTsplice and pSpliceExpress represent two minigene systems based on Gateway cloning, which are available through the Addgene plasmid repository. In this review, we describe the features of these two splicing reporter minigene systems. Moreover, we provide an overview of studies in which determinants of alternative splicing were investigated by using pDESTsplice or pSpliceExpress. The studies were reviewed with regard to the investigated splicing regulatory events and the experimental strategy to construct and perform a splicing reporter minigene assay. We further elaborate on how analyses on the regulation of RNA splicing offer promising prospects for gaining important insights into disease mechanisms.     

Olfactory sensitivity to group-specific substances in Atlantic salmon (Salmo salar L.)

The olfactory sensitivity of three groups of Atlantic salmon (Salmo salar L.) toward substances emanating from their own groups was studied. Thresholds were determined by electrophysiological recordings of the induced waves from the medial and lateral part of the olfactory bulb surface. The intestine contained more potent olfactory substances on a gram per liter basis than skin mucus, urine, or amino acids. Chemical fractions were obtained from a parallel study on the nature of the naturally occurring substances by Stabell et al. (1982). A retarded fraction from chromatography on a Sephadex G-25 column contained the most potent material. The most potent fractions of the intestinal content evoked responses mainly in the medial part of the olfactory bulb, whereas the lateral part responded to amino acids. The results suggest that all salmon smolts of the waterways contribute to an odor trail in the coastal currents, thus facilitating the odor-dependent migration of the mature salmon.     

Characterization of U/Pu particles originating from the nuclear weapon accidents at Palomares, Spain, 1966 and Thule, Greenland, 1968

Following the USAF B-52 bomber accidents at Palomares, Spain in 1966 and at Thule, Greenland in 1968, radioactive particles containing uranium (U) and plutonium (Pu) were dispersed into the environment. To improve long-term environmental impact assessments for the contaminated ecosystems, particles from the two sites have been isolated and characterized with respect to properties influencing particle weathering rates. Low (239)Pu/(235)U (0.62-0.78) and (240)Pu/(239)Pu (0.055-0.061) atom ratios in individual particles from both sites obtained by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) show that the particles contain highly enriched U and weapon-grade Pu. Furthermore, results from electron microscopy with Energy Dispersive X-ray analysis (EDX) and synchrotron radiation (SR) based micrometer-scale X-ray fluorescence (micro-XRF) 2D mapping demonstrated that U and Pu coexist throughout the 1-50 microm sized particles, while surface heterogeneities were observed in EDX line scans. SR-based micrometer-scale X-ray Absorption Near Edge Structure Spectroscopy (micro-XANES) showed that the particles consisted of an oxide mixture of U (predominately UO(2) with the presence of U(3)O(8)) and Pu ((III)/(IV), (IV)/(V) or (III), (IV) and (V)). Neither metallic U or Pu nor uranyl or Pu(VI) could be observed. Characteristics such as elemental distributions, morphology and oxidation states are remarkably similar for the Palomares and Thule particles, reflecting that they originate from similar source and release scenarios. Thus, these particle characteristics are more dependent on the original material from which the particles are derived (source) and the formation of particles (release scenario) than the environmental conditions to which the particles have been exposed since the late 1960s.     

Potential remobilization of 137Cs, 60Co, 90Tc,and 90Sr from contaminated Mayak sediments in river and estuary environments

Following 50 years of nuclear production at Mayak PA, sediments in storage reservoirs are significantly contaminated. Dam failure or flooding could potentially transport large amounts of sediments, via rivers, to the Ob estuary and Kara Sea. The objectives of this work were to investigate fresh and seawater remobilization of 137Cs, 50Co, 99Tc, and 90Sr from contaminated Reservoir 10 sediments. Sediments were extracted sequentially using synthetic Techa freshwater, seawater, and chemical reagents with increasing dissolution powers. 137Cs and 90Sr freshwater distribution coefficients (apparent Kd) agreed quite well with published values; values for 99Tc were higher and values for 60Co were lower than expected. In seawater, mean apparent Kd values decreased by 94, 77, 48, and 73% (137Cs, 60Co, 99Tc, and 90Sr, respectively), indicating increased radionuclide mobility. Remobilization in seawater was 5, 15, 1, and 23% of total activities (i.e., releases of 165, 11, 0.3, and 170 kBq kg(-1) d.w.) for 137Cs, 60Co, 99Tc, and 90Sr, respectively. 137Cs and 99Tc were strongly bound to sediments (60% and 80%, respectively). 60Co and 90Sr were more mobile (70% reversibly bound). In conclusion, Mayak Reservoir sediments could potentially contaminate the Ob estuary due to remobilization of sediment-held radionuclides upon contact with seawater.