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Kenji Ichimura Eihachi Takamura 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1992,320(3):604-605
A hydrogenated caesium-graphite intercalation compound, of which the Raman spectrum indicates its second-stage structure, is found to be formed by exposure of CsC8 to atomic hydrogen generated on a hot tungsten filament. 相似文献
3.
An automatic optical through-hole inspection system for ultrahigh density printed wiring boards (PWBs) using leakage light detection has been developed. To detect the dim leakage light emitted from the through-hole defect, we enhanced the sensitivity of the light detector 150 times using a microchannel plate tube. However, the tube caused two problems: stray light sensing and image distortion. To solve these problems, we optically isolated the sensing optics and developed a distortion correction method. We have developed a prototype system that can detect a defect as small as 100 m. 相似文献
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Masashi Kato Tomonari Yasuda Keiko Miyake Masaya Ichimura Tomoaki Hatayama 《International Journal of Hydrogen Energy》2014
Solar-to-hydrogen conversion efficiencies of water-splitting photochathodes using epitaxially grown p-type 4H-, 6H- and 3C-SiC were estimated in a two-electrode system without applying any external bias. By using electrode materials with small oxygen overpotentials as counter electrodes, the photocurrent became comparable to that observed in a three-electrode system with a suitable bias. Estimated efficiencies seem to depend on the bandgap of the SiC polytypes. For the 3C-SiC, the obtained efficiency was 0.38%, which is so far the highest value reported for SiC. We confirmed that the hydrogen volumes estimated from the photocurrent were almost the same as actual volumes observed by gas chromatography. 相似文献
6.
Inside Cover: Biosynthetic Gene Cluster for Surugamide A Encompasses an Unrelated Decapeptide,Surugamide F (ChemBioChem 18/2016)
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7.
Sone T Nagamori E Ikeuchi T Mizukami A Takakura Y Kajiyama S Fukusaki E Harashima S Kobayashi A Fukui K 《Journal of Bioscience and Bioengineering》2002,94(1):87-91
We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants. 相似文献
8.
Protective effects of chondroitin sulfate proteoglycans (CSPGs) from rat's brain against delayed cell death induced by excitatory amino acids were examined in cultured neurons of the rat. CSPGs reduced delayed neuronal death induced by 10 min exposure to glutamate at a concentration between 100 microM and 1 mM when lactate dehydrogenase activity of culture medium was assayed 24 h after the exposure. CSPGs also protected neuronal death induced by 200 microM N-methyl-D-aspartate (NMDA), kainate or 100 microM alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). CSPGs reduced death of cortical and hippocampal neurons even when they were administered at 2 h, but not 6 and 12 h, after the exposure to glutamate. These results indicate that CSPGs may have a neuroprotective action against acute noxious conditions in the brain. 相似文献
9.
Higashi T Nagamori E Sone T Matsunaga S Fukui K 《Journal of Bioscience and Bioengineering》2004,97(3):191-195
The direct transfer of genetic materials into mammalian cells is an indispensable technique. We have developed calcium alginate (CA) microbeads which can deliver plasmid DNAs and yeast artificial chromosomes into plant and yeast cells. In this paper, we demonstrate the effective transfection of mammalian cells by CA microbeads immobilizing plasmid DNAs. The transfection was performed using the pEGFP-C1 plasmid containing the cytomegalovirus (CMV) promoter and enhanced green fluorescent protein (EGFP) gene. The transient expression of EGFP was observed 24 h after transfection. The expression efficiency was maximum when the concentration of sodium alginate was 1% and the amount of plasmid DNA was increased to 100 microg. The expression efficiency of our method using CA microbeads is 2-10 times higher than that of the polyethylene glycol (PEG) method. Our results suggest that the CA microbead mediated transfection of mammalian cells effectively delivers genetic materials into mammalian suspension cells. 相似文献
10.
A general steady state mathematical model for fin-and-tube heat exchanger based on graph theory 总被引:9,自引:0,他引:9
Jian Liu WenJian Wei GouLiang Ding Chunlu Zhang Masaharu Fukaya Kaijian Wang Takefumi Inagaki 《International Journal of Refrigeration》2004,27(8):965-973
Fin-and-tube heat exchangers are widely used in air conditioners, chillers, etc. A lot of factors, including arrangement of refrigerant circuits, configure specification of fins and tubes, and operating conditions, have significant influence on the performance of fin-and-tube heat exchangers. For the purpose of fast design of high performance heat exchangers, a simulator reflecting the influence of these factors is necessary. In this paper, a general steady state mathematic model based on the graph theory is presented. With the help of the directed graph and graph-based traversal methods (Breadth-first search and Depth-first search), this model is capable to describe any flexible refrigerant circuit arrangement, and quantify the refrigerant distribution in the refrigerant circuit and heat conduction through fins. An alternative iteration method is also developed to solve the conservation equations, which can shorten the simulating time effectively. The model is verified with the experimental results, and the maximum error is within ±10.0%. A simulator based on this model has been used for designing practical fin-and-tube heat exchangers. 相似文献