Free Radical Scavenging Activity of Aqueous and Ethanolic Extract of Brassica oleracea L. var. italica

In this study, antioxidant activities of aqueous and ethanolic extracts of Brassica oleracea L. var. italica were investigated. The antioxidant properties of both extracts of Brassica oleracea L. var. italica were evaluated using different antioxidant tests, including 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide radical scavenging, inhibition of microsomal lipid peroxidation, reduction of power, and metal ion chelating activities. Inhibition of superoxide scavenging by aqueous and ethanolic extracts showed an IC₅₀ of 0.93 and 0.25 mg/ml, respectively. Metal ion chelation showed an IC₅₀ of 0.35 mg/ml of both the extracts and was equipotent to positive control, ethylenediamine tetra-acetic acid. The ethanolic extract of Brassica oleracea L. var. italica exhibited higher antioxidant activity in DPPH radical and superoxide anion scavenging than that of aqueous extract. The results obtained in the in vitro models clearly suggest that, Brassica oleracea L. var. italica is a natural source for antioxidants, which could serve as a nutraceutical with potential applications in reducing the level of oxidative stress and related health benefits. However, comprehensive studies need to be conducted to ascertain the in vivo safety of such extracts in experimental animal models.     

Structure prediction of subtilisin BPN' mutants using molecular dynamics methods

In this paper we describe the achievements and pitfalls encounteredin doing structure predictions of protein mutants using moleculardynamics simulation techniques in which properties of atomsare slowly changed as a function of time. Basically the methodconsists of a thermodynamic integration (slow growth) calculationused for free energy determination, but aimed at structure prediction;this allows for a fast determination of the mutant structure.We compared the calculated structure of the mutants Met222Ala,Met222Phe and Met222Gln of subtilisin BPN' with the respectiveX-ray structures and found good agreement between predictedand X-ray structure. The conformation of the residue subjectto the mutation is relatively easy to predict and is mainlydetermined by packing criteria. When the side chain has polargroups its exact orientation may pose problems; long-range Coulombinteractions may generate a polarization feedback involvingsystem relaxation times beyond the simulation time. Changesinduced in the environment are harder to predict using thismethod. In particular, rearrangement of the hydration structurewas difficult to predict correctly, probably because of thelong relaxation times. In all conversions made the changes observedin the environment were found to be history-dependent and inparticular the hydrogen bonding patterns provided evidence formetastable substates. In all cases the structure predicted wascompared with available kinetic data and the reduced activitycould be explained in terms of changes in the configurationof the active site.     

Low-temperature, shear-induced tribofilm formation from dimethyl disulfide on copper

The frictional properties of a sliding copper-copper interface exposed to dimethyl disulfide (DMDS) are measured in UHV under conditions at which the interfacial temperature rise is <1 K. A significant reduction in friction is found from the clean-surface values and sulfur is found on the surface and below the surface in the wear scar region by Auger spectroscopy. Because the interfacial temperature rise under the experimental conditions used to measure friction is very small, tribofilm formation is not thermally induced. The novel, low-temperature tribofilm formation observed here is ascribed to a shear-induced intermixing of the surface layer(s) with the subsurface region as suggested using previous molecular dynamics simulations. Although the tribofilm contains predominantly sulfur, a small amount of carbon is also found in the film.     

Preparation and solubility of metal soaps of wool wax acids

The preparation and solubility determination of the cupric, magnesium, nickel, cobalt, cadmium, lead, barium, manganous, ferric, and chromic soaps of the wool wax acid fraction are described. Solubilities, at 25°, were determined in: ethanol, methanol, isopropyl alcohol, acetone, ethyl acetate, carbon tetrachloride, and petroleum ether. Barium and cadmium soaps of hydroxy and nonhydroxy acid fractions, obtained by partitioning the whole wool wax acid fraction, were also prepared and subjected to the same study. Presented at the Spring Meeting of the American Oil Chemists' Society, St. Louis, Missouri, May 1–3, 1961. Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.     

The curriculum in and for a democratic society 1

The purpose of this article is to offer an analysis of the political and social role of the curriculum in a modern democratic society. It seeks to show how the assumptions embedded in inherited curriculum ideologies are impeding the potential of the curriculum in modern democratic societies from becoming a curriculum 'for democracy', that is a curriculum which would be constitutive of a more democratic form of social life.     

Prandtl–Tomlinson-Type Models for Coupled Molecular Sliding Friction: Chain-Length Dependence of Friction of Self-assembled Monolayers

Tribology Letters - Most asteroids with a diameter larger than $$sim 300 {mathrm{m}}$$ are rubble piles, i.e., consisting of more than one solid object. All asteroids are rotating but almost...     

Maintaining frequent closed itemsets over a sliding window

In this paper, we study the incremental update of Frequent Closed Itemsets (FCIs) over a sliding window in a high-speed data stream. We propose the notion of semi-FCIs, which is to progressively increase the minimum support threshold for an itemset as it is retained longer in the window, thereby drastically reducing the number of itemsets that need to be maintained and processed. We explore the properties of semi-FCIs and observe that a majority of the subsets of a semi-FCI are not semi-FCIs and need not be updated. This finding allows us to devise an efficient algorithm, IncMine, that incrementally updates the set of semi-FCIs over a sliding window. We also develop an inverted index to facilitate the update process. Our empirical results show that IncMine achieves significantly higher throughput and consumes less memory than the state-of-the-art streaming algorithms for mining FCIs and FIs. IncMine also attains high accuracy of 100% precision and over 93% recall.