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Based on the details in the case, the analysis method of methamphetamine was built up, which is supposed to be used for in vitro material taken from the criminal scene. It is suggested that GC-FID is first used for methamphetamine concentration scanning. Thus, according to the results, the sample could be concentrated or diluted for the next GC/MS analysis. Using this procedure, the satisfying result was achieved. 相似文献
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Analysis of the seized amphetamine was performed using GC/MS. The method was investigated in terms of extraction methods, aiming at the effective removal of adulterants in high concentration, which interfere amphetamine analysis. This research is supposed to be useful for analyzing the seized drags similar with the one in this paper. 相似文献
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采用纤维素酶法提取蜂花粉可溶性膳食纤维,分析其理化特性,并与酸提、碱提产品进行对比。结果表明,酶法提取蜂花粉可溶性膳食纤维最优工艺条件为:酶解温度50℃,酶解时间2h,酶用量2.5%,pH 4。在该条件下,可溶性膳食纤维得率为4.11%,产品呈乳白色、粉体,荷花蜂花粉特有气味浓郁,膨胀力2.03 mL/g、持水力1.01 g/g、水溶性91.26%,含有甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7种单糖组分,具有多糖特征吸收峰。与碱提、酸提工艺相比,酶提产品具有较好水溶性和感官品质,更易于应用在饮料类食品的开发中。 相似文献
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以纤维素酶酶解提取、酸溶液提取、碱溶液提取3种方法提取的荷花蜂花粉可溶性膳食纤维(bee pollen soluble dietary fiber,BPSDF)为研究对象,探究提取方法对BPSDF多酚含量及常见致病菌、有益菌生长的影响。结果表明,BPSDF中多酚主要以游离态的形式存在,纤维素酶酶解提取、酸溶液提取、碱溶液提取的游离酚分别占其总酚的81.16%,80.34%,69.59%;纤维素酶解提取的游离酚、总酚含量相对较高;碱溶液提取的结合酚含量相对较高。提取方法不同BPSDF游离酚、结合酚和总酚含量均差异显著,但对BPSDF中游离酚、结合酚的多酚组分影响较小。3种方法提取的BPSDF对大肠杆菌、金黄色葡萄球菌、白色念珠球菌均有较好的抑制作用,相对来说纤维素酶酶解提取的BPSDF的抑菌作用最好;3种方法提取的BPSDF对双歧杆菌和嗜酸乳杆菌有一定的促进作用,且对供试有害菌的抑制作用优于对供试有益菌的促进作用。 相似文献
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To achieve higher antibiotic streptolydigin productivity through metabolic regulation, propionate was fed during the fermentation of Streptomyces lydicus AS 4.2501. The effects of propionate feeding on streptolydigin production and intracellular fluxes were investigated. The highest streptolydigin production yield of 95.10mg·L^-1 was obtained when 2mmol·L^-1 of sodium propionate was added at 60h of cultivation into shake-flask culture. This yield is 23.06% higher when compared to that of a batch culture without propionate supplementation. It was also found that when propionate was added, much more organic acids were excreted. Metabolic flux analysis was performed and it demonstrated that the carbon fluxes of the pentose phosphate pathway and the anaplerotic reaction were significantly increased after propionate feeding. The carbon flux from pyruvate to acetyl-CoA was determined to be 24.7, which was 12.27% higher than that in the batch culture. This study indicated that the glucose-6-phosphate and pyruvate nodes were potential bottlenecks for increasing streptolydigin productivity. Potential targets and strategies that could be manipulated through genetic and process engineering to increase the production of streptolydigin were also suggested. 相似文献
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