A method for a robust optimization of joint product and supply chain design

This paper proposes a method for finding a robust solution to the problem of joint product family and supply chain design. Optimizing product design and the supply chain network at the same time brings substantial benefits. However, this approach involves decisions that can generate uncertainties in the long term. The challenge is to come up with a method that can adapt to most possible environments without straying too far from the optimal solution. Our approach is based on the generation of scenarios that correspond to combinations of uncertain parameters within the model. The performance of designs resulting from these scenario optimizations are compared to the performance of each of the other design scenarios, based on their probability of occurrence. The proposed methodology will allow practitioners to choose a suitable design, from the most profitable to the most reliable.     

Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras

In an interferometer-based fluorescence microscope, a beam splitter is often used to combine two emission wavefronts interferometrically. There are two perpendicular paths along which the interference fringes can propagate and normally only one is used for imaging. However, the other path also contains useful information. Here we introduced a second camera to our interferometer-based three-dimensional structured-illumination microscope (I(5)S) to capture the fringes along the normally unused path, which are out of phase by π relative to the fringes along the other path. Based on this complementary phase relationship and the well-defined phase interrelationships among the I(5)S data components, we can deduce and then computationally eliminate the path length errors within the interferometer loop using the simultaneously recorded fringes along the two imaging paths. This self-correction capability can greatly relax the requirement for eliminating the path length differences before and maintaining that status during each imaging session, which are practically challenging tasks. Experimental data is shown to support the theory.     

A survey of models and algorithms for emergency response logistics in electric distribution systems. Part I: Reliability planning with fault considerations

Emergency response operations in electric distribution systems involve a host of decision-making problems at the reliability and contingency planning levels. Those operations include fault diagnosis, fault location, fault isolation, restoration, and repair. As the first of a two-part survey, this paper reviews optimization models and solution methodologies for reliability planning problems with fault considerations related to electric distribution operations. Contingency planning problems of emergency distribution response are discussed in the second part. The present paper surveys research on determining a distribution substation single-fault capacity, reallocating excess load, configuring distribution systems, partitioning a geographical area into service territories, and locating material stores and depots.     

An experimental study for the selection of modules and facilities in a mass customization context

To design an efficient product family, designers have to anticipate the production process and, more generally, the supply chain costs. But this is a difficult problem, and designers often propose a solution which is subsequently evaluated in terms of logistical costs. This paper presents a design problem in which the product and the supply chain design are considered at the same time. It consists in selecting a set of modules that will be manufactured at distant facilities and then shipped to a plant close to the market for final, customized assembly under time constraints. The goal is to obtain the bill of materials for all the items in the product family, each of which is made up of a set of modules, and specifying the location where these modules will be built, in order to minimize the total production costs for the supply chain. The objective of the study is to analyze both, for small instances, the impact of the costs (fixed and variable) on the optimal solutions, and to compare an integrated approach minimizing the total cost in one model with a two-phases approach in which the decisions relating to the design of the products and the allocation of modules to distant sites are made separately.     

Structure of alpha-lytic protease complexed with its pro region

While the majority of proteins fold rapidly and spontaneously to their native states, the extracellular bacterial protease alpha-lytic protease (alphaLP) has a t(1/2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1). AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction. Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9). In the absence of Pro, alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report the crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP. The C-shaped Pro surrounds the C-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier.     

The solution of a dosimetry problem caused by a mercury shutter

Replies to L. E. Beutler et al (see record 1973-31675-001) who presented data in defense of using the number of patients rather than the number of therapists as the unit of analysis for assessing the reliability of process psychotherapy variables. The present paper presents data suggesting that the Beutler et al interpretation of their research findings is not valid. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Activation of a retroviral membrane fusion protein: soluble receptor-induced liposome binding of the ALSV envelope glycoprotein

Cellular aspects of remodeling in intact arteries have not been fully investigated, mainly due to the lack of an appropriate methodology that allows for simple measurements. The aim of this study was to develop a method based on laser scanning confocal microscopy (LSCM), compare it with previous methodology, and apply it to the study of remodeling in hypertension. The morphology of mesenteric resistance arteries from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto rats (WKY) was determined with wire myography on one segment with a standardized diameter setting (0.9[d100]) and with perfusion myography on a second segment from the same artery at the calculated equivalent pressure. The second segments were stained with the nuclear dye Hoechst 33342 (live tissue) or propidium iodide (fixed tissue) and measured with LSCM and MetaMorph software. Compared with wire myography, perfusion myography showed similar differences from those previously reported. Compared with LSCM, perfusion myography showed a similar lumen but significantly smaller wall thickness in both live and fixed tissue, probably due to measurement underestimation. In the study with LSCM, arteries from SHRSP compared with those from WKY showed (1) reduced lumen, (2) altered cell density that was significantly increased in the adventitia, decreased in the media, and unchanged in the intima, (3) significantly increased medial volume, (4) significantly smaller endothelial cell nuclei, and (5) adventitial-like cells in the media. We conclude that (1) LSCM is a reliable and straightforward method to study morphology in intact vessels, (2) it provides new information on the cellular changes in remodeling, (3) adventitia might play an active role in the process of remodeling in hypertension, and (4) endothelium "remodels" in hypertension.