Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor

Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae.     

Supporting primary students' learning of fraction conceptual knowledge through digital games

With the advent of mobile technologies, well-designed fraction apps can be used to help children gain fraction knowledge, a challenging topic for both teachers and students. The present pilot study adopted a quasi-experimental design to investigate whether children can learn fraction concepts equally well if half of the lesson time (20 min) is replaced with game-based learning. Keeping the total lesson time (40 min) identical, the control group (N = 33) received traditional instruction, and the experimental group (N = 32) was presented with a blended learning approach spending half of the class time (20 min) playing tablet-based fraction games, where each of the learners had their own tablet. The results suggested that in the posttest, the experimental group achieved similar learning gains to the control group and appear to have achieved better performance in the transfer test than the control group. This paper also discusses the efficiency of game-based learning, the mechanism of how fraction games might enhance learning, and the potential of integrating game-based learning in educational settings.     

深水井控中节流管线摩阻分析及压井方法

在海洋深水钻井中，防喷器组一般安装在海底，当发生井涌进行压井作业时．由于节流管线较长、内径较小，钻井液在节流管线内存在高摩阻损失，作用在井底的压力往往大于地层的破裂压力．从而引起压井过程的复杂化，甚至造成压井作业的失败。介绍了一种新的压井方法附加流量法，在返出的节流管线流体中混入低密度流体，降低节流管线内流体的粘度和密度，从而实现安全压井；详细介绍了压井方法和步骤。数据分析表明，通过对压井参数进行优化选择能够降低节流管线中的压耗。     

双膦胺镍-甲基铝氧烷体系催化苯乙烯聚合

研究了具有新型结构的双膦胺镍配合物N,N-双(二苯膦基)-对甲氧基苯胺二氯化镍-甲基铝氧烷(PNP-N i-MAO)催化体系对苯乙烯聚合的催化性能,考察了聚合温度、n(A l)∶n(PNP-N i)、PNP-N i的浓度和苯乙烯的浓度对催化活性、苯乙烯转化率、聚苯乙烯相对分子质量及其分布的影响,并用核磁共振和凝胶色谱对聚苯乙烯的结构进行了表征。实验结果表明,在聚合温度25℃、聚合时间1h、n(A l)∶n(PNP-N i)=300、c(苯乙烯)=2.3m ol/L、c(PNP-N i)=0.4mm ol/L、甲苯为溶剂的适宜条件下,苯乙烯的转化率可达95%以上,催化活性达到5×105g/(m ol.h)左右。核磁共振和凝胶色谱表征结果显示,所得聚苯乙烯为无规结构,重均相对分子质量约为1×104,相对分子质量分布Mw/Mn约为2。     

抵御已知/选择明文攻击的混沌图像加密算法

论文在对现有一类典型图像混沌加密算法的分析基础上,提出了一种改进的图像混沌加密算法。该算法引入小波变换,可以有效地克服一些混沌加密算法不能抵御已知/选择明文攻击的缺陷。     

煤层气利用新方案

在介绍煤层气的常规利用方案的基础上，提出了两种利用煤层气的新方案：一是采用变压吸附技术，纯化瓦斯气，将低浓度瓦斯气浓缩为高浓度瓦斯气，加压后成为CNG，用作CNG气源或较远距离城市的天然气气源；二是在第一种方案的基础上，将高浓度瓦斯气全部采用深冷法加以液化，生产LNG，并将LNG运送至更远距离用作LNG加气站、CNG加气站气源。最后还以松藻煤电公司为例，作了新方案的工程预算     

真空铸造高强度C/Mg复合材料

本文介绍用真空铸造法制造抗拉强度超过1000MPa的高强度C/Mg复合材料。将T300纤维经C-Si-O梯度涂层处理后单向排布于型腔内,在真空下注入ZM-5合金液,凝固后即得C/Mg复合材料零件或试样。试样的抗拉强度在纤维体积分数为0.35时达1050MPa。本文讨论C-Si-O梯度涂层对获得高强度复合材料的作用。