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1.
2.
[3H]Platelet-activating factor (PAF) binding to rabbit platelet membranes was examined before and after 20 min preincubation
at 25°C in the presence of PAF, lysoPAF, or of five different PAF receptor antagonists (L 652731, BN 52021, WEB 2086, BN 52111
and BN 52115). When platelet membranes were not washed after preincubation with PAF or PAF antagonists, no significant specific
binding of [3H]PAF was observed, which suggests full occupancy of the binding sites. When membranes were extensively washed, full recovery
of specific [3H]PAF binding was attained with L 652731 and partial recoveries (60%, 55% and 30%) were reached with BN52021, WEB 2086 and
PAF, respectively; no recovery was seen with the dioxolanes BN 52111 and BN 52115. Scatchard analysis of the binding data
indicated that no significant change in the dissociation constant (Kd) and maximum number of binding sites (Bmax) occurred after preincubation of platelet membrane with L 652731, whereas a reduction of Bmax was observed when PAF and BN 52021 were measured. When platelet membranes were preincubated with WEB 2086, Bmax and Kd significantly increased. The data suggest differing binding properties for PAF and the PAF antagonists. Some of the PAF antagonists
may tightly bind to the PAF receptor site(s) and/or irreversibly modify or downregulate PAF recognition sites. Our results
also suggest that the interaction of PAF receptor antagonists with PAF receptor can be divided into at least two components,
namely a reversible component and an irreversible one.
Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl
Ether Lipids, Tokyo, Japan, May 1989. 相似文献
3.
The two main proteic constituents of the human Apo-bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction
(APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5
mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis
and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum
directed solely against the APF. 相似文献
4.
Gloria Galan-Marin Enrique Merida-Casermeiro Domingo Lopez-Rodriguez 《Neural Processing Letters》2007,26(2):133-143
Detection of isomorphism among kinematic chains is essential in mechanical design, but difficult and computationally expensive.
It has been shown that both traditional methods and previously presented neural networks still have a lot to be desired in
aspects such as simplifying procedure of identification and adapting automatic computation. Therefore, a new algorithm based
on a competitive Hopfield network is developed for automatic computation in the kinematic chain isomorphism problem. The neural
approach provides directly interpretable solutions and does not demand tuning of parameters. We have tested the algorithm
by solving problems reported in the recent mechanical literature. Simulation results show the effectiveness of the network
that rapidly identifies isomorphic kinematic chains. 相似文献
5.
JB Rodriguez L Zhong R Docampo Z Wimmer EG Gros 《Canadian Metallurgical Quarterly》1998,8(22):3257-3260
Several compounds, structurally related to the insect growth regulator Fenoxycarb, exhibited interesting inhibition action to control proliferation of Trypanosoma cruzi, the parasite responsible for Chagas' disease. Some of these drugs were shown to be potent growth inhibitors of this parasite. All of these drugs had previously presented juvenoid activity on several non-related bug species such as Tenebrio molitor, Galleria mellonella, Dysdercus cingulatos, and Pyrrhocoris apterus. 相似文献
6.
The phospholipids present in the intestinal lumen of rats following ingestion of triglycerides are of biliary origin. They consist of lecithins accompanied by a small proportion of lysolecithins. Their behavior in comparison with the other lipid constituents of the intestinal content was studied by subjecting the latter to gel filtration on an agarose column in the presence of a solution of 6 mM sodium taurocholate in 0.1 M NaCl. Part of the phospholipids is present with the triglycerides and diglycerides in the emulsified phase excluded from the gel where pancreatic lipase and colipase also are found. The remainder is found in optically clear fractions containing fatty acids, monoglycerides, and bile salts. These fractions are eluted at 2.0 column volumes, while mixed fatty acids, monoglycerides, bile salts micelles emerge from the column at 2.4 column volumes in the same chromatographic conditions. This difference in behavior may be explained by the presence of biliary lecithins. This presence could have an important bearing upon the mucosal uptake of the lipolysis products of triglycerides. 相似文献
7.
M.C. García-Gutiérrez A. Nogales C. Domingo G. Broza K. Schulte T.A. Ezquerra 《Polymer》2006,47(1):341-345
We report the appearance of a novel self-assembling of a fraction of single-wall carbon nanotubes (SWCNT) within a SWCNT-polymer nanocomposite subjected to flow fields upon injection molding processing. By combining X-ray diffraction and Raman spectroscopy techniques, both working on a microfocus fashion, we probe that a fraction of the thinnest SWCNT self-assembles into a rectangular lattice in the sample regions where the shear stress induces the highest levels of nanotube aggregation. Additionally, we demonstrate that a modest amount in weight of nanotubes is enough to template the morphology of crystallization during flow providing a method to obtain a highly desirable fiber-like morphology. 相似文献
8.
Why Phenolic Acids Are Unlikely Primary Allelochemicals in Rice 总被引:21,自引:0,他引:21
Olofsdotter M Rebulanan M Madrid A Dali W Navarez D Olk DC 《Journal of chemical ecology》2002,28(1):229-242
Allelopathy in rice (Oryza sativa, L.) effective against weeds has been found in about 3.5% of tested rice germplasm in both laboratory and field experimentation. However, the allelochemicals responsible for growth inhibition of rice-associated weeds have not yet been identified. In the literature, phenolic acids are often mentioned as putative allelochemicals. If phenolic acids commonly reach growth inhibitory concentrations in rice ecosystems, it must be expected that the degree of tolerance to phenolic acids will vary among traditional rice cultivars or plant species adapted to rice environments having inherently different phenolic acid concentrations. Phenolic acids concentrations are normally greater in submerged than in aerobic soils. A dose–response study, however, showed that seedlings of rice cultivars adapted to submerged anaerobic soils did not have higher level of tolerance against p-hydroxybenzoic acid than did seedlings of varieties adapted to aerobic upland soils. Moreover, traditional rice cultivars had no greater tolerance than did improved cultivars that were recently bred for traits other than tolerance of phenolic acids. Similarly, there were no differences in tolerance of p-hydroxybenzoic acid between two Echinochloa weed species adapted to either anaerobic or aerobic growth conditions. Thus, neither the rice cultivars nor weed species had evolved different tolerance levels against the phenolic acid. However, all rice cultivars had significantly greater tolerance of p-hydroxybenzoic acid than did either weed species. In a second experiment, the rates at which rice plants released phenolic acids into solution cultures were measured for at least one month, the time period of greatest allelopathic activity following planting under field conditions. The maximum release rate of phenolic acids during the first month of growth was approximately 10 g/plant/day. At a conventional plant density, the release rate of phenolic acids would be approximately 1 mg/m2day. This order of release rate cannot provide concentrations remotely close to phytotoxic levels determined for these rice cultivars and weed species. The results presented in this paper do not preclude the possibility that phenolic acids might be one component in a mixture of chemicals that, when present simultaneously, are allelopathic. 相似文献
9.
In recent years, X-ray screening systems have been used to safeguard environments in which access control is of paramount importance. Security checkpoints have been placed at the entrances to many public places to detect prohibited items such as handguns and explosives. Human operators complete these tasks because automated recognition in baggage inspection is far from perfect. Research and development on X-ray testing is, however, ongoing into new approaches that can be used to aid human operators. This paper attempts to make a contribution to the field of object recognition by proposing a new approach called Adaptive Sparse Representation (XASR+). It consists of two stages: learning and testing. In the learning stage, for each object of training dataset, several patches are extracted from its X-ray images in order to construct representative dictionaries. A stop-list is used to remove very common words of the dictionaries. In the testing stage, test patches of the test image are extracted, and for each test patch a dictionary is built concatenating the ‘best’ representative dictionary of each object. Using this adapted dictionary, each test patch is classified following the Sparse Representation Classification methodology. Finally, the test image is classified by patch voting. Thus, our approach is able to deal with less constrained conditions including some contrast variability, pose, intra-class variability, size of the image and focal distance. We tested the effectiveness of our method for the detection of four different objects. In our experiments, the recognition rate was more than 97 % in each class, and more than 94 % if the object is occluded less than 15 %. Results show that XASR+ deals well with unconstrained conditions, outperforming various representative methods in the literature. 相似文献
10.
Francisco Almeida Domingo Giménez Jose J. López-Espín 《The Journal of supercomputing》2011,58(3):292-301
This paper presents a parameterized shared-memory scheme for parameterized metaheuristics. The use of a parameterized metaheuristic
facilitates experimentation with different metaheuristics and hybridation/combinations to adapt them to the particular problem
we are working with. Due to the large number of experiments necessary for the metaheuristic selection and tuning, parallelism
should be used to reduce the execution time. To obtain parallel versions of the metaheuristics and to adapt them to the characteristics
of the parallel system, a unified parameterized shared-memory scheme is developed. Given a particular computational system
and fixed parameters for the sequential metaheuristic, the appropriate selection of parameters in the unified parallel scheme
eases the development of parallel efficient metaheuristics. 相似文献