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1.
Tumor cell aggregation is critical for cell survival following the loss of extracellular matrix attachment and dissemination. However, the underlying mechanotransduction of clustering solitary tumor cells is poorly understood, especially in non-small cell lung cancers (NSCLC). Here, we examined whether cell surface protrusions played an important role in facilitating the physical contact between floating cells detached from a substrate. We employed poly-2-hydroxyethyl methacrylate-based 3D culture methods to mimic in vivo tumor cell cluster formation. The suprastructural analysis of human NSCLC A549 cell spheroids showed that finger-like protrusions clung together via the actin cytoskeleton. Time-lapse holotomography demonstrated that the finger-like protrusions of free-floating cells in 3D culture displayed exploratory coalescence. Global gene expression analysis demonstrated that the genes in the organic hydroxyl transport were particularly enriched in the A549 cell spheroids. Particularly, the knockdown of the water channel aquaporin 3 gene (AQP3) impaired multicellular aggregate formation in 3D culture through the rearrangement of the actomyosin cytoskeleton. Moreover, the cells with reduced levels of AQP3 decreased their transmigration. Overall, these data indicate that cell detachment-upregulated AQP3 contributes to cell surface protrusions through actomyosin cytoskeleton remodeling, causing the aggressive aggregation of free-floating cells dependent on the property of the substratum and collective metastasis. 相似文献
2.
Maenghyo Cho Hee Yuel Roh 《International journal for numerical methods in engineering》2003,56(1):81-115
In the present study first‐order shear deformable shell finite elements based on general curvilinear co‐ordinates are proposed. For the development of the present shell elements, a partial mixed variational functional with independently assumed strains is provided in order to avoid the severe locking troubles known as transverse shear and membrane lockings. Bubble functions are included in the shape function of displacement to improve the performance of the developed element. The proposed assumed strain four‐ and nine‐node elements based on the general tensor shell theory provide an efficient linkage framework for shell surface modelling and finite element analysis. In the several benchmark problems, the present shell elements with exact geometric representations demonstrate their performance compared to previously reported results. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
3.
Dong-Soo Yoon Jae Sung Roh Sung-Man Lee Hong Koo Baik 《Journal of Electronic Materials》2003,32(8):890-898
The effect of a thin RuOx layer formed on the Ru/TiN/doped poly-Si/Si stack structure was compared with that on the RuOx/TiN/doped poly-Si/Si stack structure over the post-deposition annealing temperature ranges of 450–600°C. The Ru/TiN/poly-Si/Si
contact system exhibited linear behavior at forward bias with a small increase in the total resistance up to 600°C. The RuOx/TiN/poly-Si/Si contact system exhibited nonlinear characteristics under forward bias at 450°C, which is attributed to no
formation of a thin RuOx layer at the RuOx surface and porous-amorphous microstructure. In the former case, the addition of oxygen at the surface layer of the Ru film
by pre-annealing leads to the formation of a thin RuOx layer and chemically strong Ru-O bonds. This results from the retardation of oxygen diffusion caused by the discontinuity
of diffusion paths. In particular, the RuOx layer in a nonstoichiometric state is changed to the RuO2-crystalline phase in a stoichiometric state after post-deposition annealing; this phase can act as an oxygen-capture layer.
Therefore, it appears that the electrical properties of the Ru/TiN/poly-Si/Si contact system are better than those of the
RuOx/TiN/poly-Si/Si contact system. 相似文献
4.
Jeongjin Roh 《Circuits and Systems II: Express Briefs, IEEE Transactions on》2005,52(9):591-595
A new error amplifier is presented for fast transient response of dc-dc converters. The amplifier has low quiescent current to achieve high power conversion efficiency, but it can supply sufficient current during large-signal operation. Two comparators detect large-signal variations, and turn on extra current supplier if necessary. The amount of extra current is well controlled, so that the system stability can be guaranteed in various operating conditions. The simulation results show that the new error amplifier achieves significant improvement in transient response than the conventional one. 相似文献
5.
Optimal observer-based wheelbase preview regulator problem is investigated for active vehicle suspension systems. It is shown
that the problem reduces to the classical linear quadratic Gaussian problem, whose solution is well defined, by augmenting
dynamics of system and road inputs. The resulting optimal controller is in the form of augmented state feedback controller
and this augmented state is estimated by Kalman-Bucy filter using dynamics of the augmented system. Numerical examples of
a half car model are given to verify the performance improvement achievable with the proposed controller. 相似文献
6.
Genetically optimized fuzzy polynomial neural networks with fuzzy set-based polynomial neurons 总被引:2,自引:0,他引:2
In this paper, we propose and investigate a new category of neurofuzzy networks—fuzzy polynomial neural networks (FPNN) endowed with fuzzy set-based polynomial neurons (FSPNs) We develop a comprehensive design methodology involving mechanisms of genetic optimization, and genetic algorithms (GAs) in particular. The conventional FPNNs developed so far are based on the mechanisms of self-organization, fuzzy neurocomputing, and evolutionary optimization. The design of the network exploits the FSPNs as well as the extended group method of data handling (GMDH). Let us stress that in the previous development strategies some essential parameters of the networks (such as the number of input variables, the order of the polynomial, the number of membership functions, and a collection of the specific subset of input variables) being available within the network are provided by the designer in advance and kept fixed throughout the overall development process. This restriction may hamper a possibility of developing an optimal architecture of the model. The design proposed in this study addresses this issue. The augmented and genetically developed FPNN (gFPNN) results in a structurally optimized structure and comes with a higher level of flexibility in comparison to the one we encounter in the conventional FPNNs. The GA-based design procedure being applied at each layer of the FPNN leads to the selection of the most suitable nodes (or FSPNs) available within the FPNN. In the sequel, two general optimization mechanisms are explored. First, the structural optimization is realized via GAs whereas the ensuing detailed parametric optimization is carried out in the setting of a standard least square method-based learning. The performance of the gFPNN is quantified through experimentation in which we use a number of modeling benchmarks—synthetic and experimental data being commonly used in fuzzy or neurofuzzy modeling. The obtained results demonstrate the superiority of the proposed networks over the models existing in the references. 相似文献
7.
We evaluated the effects of tannic acid and purified perrsimmon tannin on survival and reproduction of bean bugs, Riptortus clavatus.Feeding behavior of R. clavatus was also examined on sweet (cv. Fuyu) and astringent (cv. Chongdosi) persimmon fruits. Soluble tannin in sweet persimmon fruits decreased from 3% in early June to 0.5% in late September, but it increased from 2 to 8% during the same period in astringent persimmon fruits. More bugs visited sweet than astringent persimmon. Numbers of piercing/sucking spots were higher on sweet than on astringent persimmon. When fed 1 and 3% solutions of persimmon tannin, adult bugs ingested only 64.1 and 9.5% of the amount of water ingested by those offered the control (distilled water). Amounts of persimmon tannin ingested by the adult bugs were 6.5 and 2.8 times higher at 1 and 3% tannin solutions compared to a 0.1% solution. Persimmon tannin exerted negative effects on survival and reproduction of R. clavatus at higher concentrations (1 and 3% solutions). Feeding of R. clavatus adults decreased with increasing tannin concentrations. When results from both sexes were pooled, 50% mortality was achieved at 11 and 4 days after treatment with the 1 and 3% tannin solutions, respectively. Reproduction decreased with 1% tannin, and no eggs were produced with 3% tannin solution. Tannic acid was similar in its effects on R. clavatus. All nymphs died 14, 12, and 7 days after feeding on 0.1, 1, and 3% tannic acid, respectively. Adults were less sensitive than nymphs, and their survival was not affected by 0.1% tannic acid. However. 1 and 3% tannic acid solutions were fatal. Survivorship decreased to 50% at 11 and 6 days after supplying tannin solutions of 1 and 3% concentrations. Higher concentrations (1 and 3%) resulted in reduced reproduction, as was seen with persimmon tannin. Our data may explain why R. clavatus does not invade sweet persimmon orchards until late July, when concentrations of soluble tannin are low enough to allow them to feed. 相似文献
8.
Converting random bits into random numbers is necessary for cryptographic protocols such as key agreements, public key encryptions, digital signatures and so on. In this paper, we propose the simple partial discard method and the complex partial discard method that convert random bits into random numbers. They are up to two times more efficient than standardized techniques. 相似文献
9.
Solid circulation characteristics in an internally circulating fluidized bed with orifice-type draft tube 总被引:1,自引:0,他引:1
Sang Done Kim Yo Han Kim Seon Ah Roh Dong Hyun Lee 《Korean Journal of Chemical Engineering》2002,19(5):911-916
Effects of superficial gas velocities to a draft tube, to an annulus section and particle size on the solid circulation rate
(G,) have been determined in an internally circulating fluidized bed (0.28 m I.D. × 2m high) with an orifice type draft tube.
The solid circulation rate from the draft tube to an annulus section increases with increasing gas velocities to the draft
tube(U
d
) and annulus section (Ua) and consequent increase in pressure drop across the orifice (ΔPor). However, the values ofG
s
decrease by 7–21% with increasing particle size from 86 to 288 μm. The pressure drop across the orifice increases with increasingU
d
andU
a
. However, ΔPor decreases by 5–23% with increasing particle size. To predictG
s
in an internally circulating fluidized bed, a correlation is proposed as a function of ΔPor
This paper is dedicated to Professor Dong Sup Doh on the occasion of his retirement Korea University. 相似文献
10.
The problem of environmental sustainability has been growing in recent years with an interest from both researchers and practitioners. A major gap that exists is the absence of empirical studies that addressed business value of green IS (information systems). Thus, drawing on the resource-based view of the firm and IT (information technology) business value literature, this study aims to develop a comprehensive research model of green practice-IS alignment, the key cross-functional coordination of green practices with manufacturing and marketing functions, and firm performance. The study provides a better understanding of the relationship between green practice-manufacturing coordination and green practice-marketing coordination, and how both variables mediated the relationship between green practices-IS alignment and environmental performance. The data used in this study were collected from manufacturing firms. The results show that a firm’s green practices-IS alignment had a positive effect on both green practices-manufacturing coordination and green practices-marketing coordination. In turn, both green practices-manufacturing coordination and green practices-marketing coordination are the significant predictors of environmental performance. Meanwhile, green practices-IS alignment had an indirect effect on environmental performance via both green practices-manufacturing coordination and green practices-marketing coordination. Moreover, the findings of this study show that environmental performance is an important predictor of economic performance. 相似文献