6.
Cystoseira hakodatensis is an unutilised brown algae belonging to family Sargassaceae. A crude methanol extract from the algae showed inhibitory effects on the growths of
Bacillus cereus and
Bacillus licheniformis. To isolate the major antimicrobial agent, a sequential active‐guided isolation procedure was applied: liquid–liquid extraction, column chromatography and bio‐autography. A marked antimicrobial agent (active α) was isolated in hydrophobic fraction and was determined to phenolics without carbohydrates and proteins by phytochemical test. Regarding the antimicrobial potential, the isolated active α showed better inhibitory effects against
B. cereus and
B. licheniformis at 2 and 4 times of lower concentrations (62.5 and 31.3 μg mL
?1) in comparison with epigallocatechin gallate. These results showed that
C. hakodatensis is a potential source of antimicrobial agent capable of preventing the growth of the two bacteria.
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