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The E1 and E2 reading frames of 158 cervical carcinoma samples containing human papillomavirus (HPV) 16 were mapped using polymerase chain reaction (PCR). The reading frames were amplified using primers spanning the entire genes. Of the analyzed samples, 23% showed no amplification with the E1 primers and 29% showed no amplification with the E2 primers. There was an overlap, but not complete identity, between the E1- and E2-disrupted groups. All E1- and E2-negative samples were further analyzed with primers spanning subsections of the E1 and E2 reading frames, which together covered the entire genes. Of the 35 samples negative for E1, 11 were positive in specific amplification of the 3' end of the E1 gene. Several different subsections of E2 could be amplified from most samples negative for the entire gene (37/46). Five classes of patterns were found, in which either all subsections of the E2 gene or subsections in the 5', middle, or 3' end were disrupted. Although a variable pattern of disruption/deletion in the E1-E2 area of the HPV 16 genome was found in cervical carcinoma, the 5' end disruption was the most common one in both E1 and E2. Patients with carcinomas showing disruptions in E1/E2 had a poorer survival than those without such changes, and E1 disruptions were the most important prognostically.  相似文献   
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This article addresses the development of organizational hardiness through psychological consultation emphasizing the hardiness model. A review of the hardiness construct is presented. The article moves from existential theory, in which the hardiness model is rooted, to two case examples of psychological consulting. The article also briefly gives other case examples about building organizational hardiness as a part of other consulting interventions. The emphasis in consultation designed to develop organizational hardiness is on building commitment, healthy control, an orientation toward decision and action, an attitude of finding challenge and meaning in difficult situations, and authentic community. The author concludes that hardiness is valuable both individually and organizationally and that the power of existential theory deserves renewed attention in psychology, especially in the area of consulting. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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This document outlines the expected competencies to be obtained by persons receiving training at the doctoral or postdoctoral level in consulting psychology (CP). It was prepared to assist faculty and academic administrators in designing doctoral or postdoctoral programs in CP. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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Practical training in consulting psychology is sorely lacking in most doctoral programs. This article explores the role that practicums can play in the training and development of the consultant. A program case study in clinical and consulting psychology serves as the focus. Several examples of psychological consultation involving trainees are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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