Transport and related properties of paint films. I. Composition and structure

This is the first of a series of articles on ionic and molecular transport processes and related properties of paint films. The intention is to correlate adhesion and initiation of corrosion to these relevant physical and chemical properties. Chemical formulas and structure of three vinyl–three epoxy– and one polyurethane–paint system are given in this article. Each paint has been formulated to meet appropriate military specifications. Subsequent articles will deal with dynamic mechanical properties, small angle x-ray scattering measurements of porosity, and transport properties.     

Ab initio density functional simulation of structural and electronic properties of MgO ultra-thin adlayers on the (001) Ag surface

To simulate the properties of ultrathin layers of magnesium oxide epitaxially grown on silver (001), we have adopted a periodic slab model, consisting of six layers of Ag covered on both sides with an MgO monolayer. All calculations have been performed with the 98 program. Several DFT functionals were tried and a rich basis set was adopted. The electronic and structural properties of the two bulk materials (Ag and MgO) were quite accurately reproduced. The presence of the metallic substrate was found to have an appreciable influence on the structural and electronic features of the oxide surface. In the most stable configuration (O ions directly above Ag atoms, Mg ions in the hollow sites), the surface is corrugated, and there is a net transfer of electrons from the overlayer to the metal, leading to a substantial reduction of the work function of the metal and to a decrease of the electrostatic field at the surface. The reactivity properties of the supported oxide surface have been investigated by studying the interaction of the composite material with water molecules.     

The structure of cyclin H: common mode of kinase activation and specific features

The crystal structure of human cyclin H refined at 2.6 A resolution is compared with that of cyclin A. The core of the molecule consists of two repeats containing five helices each and forming the canonical cyclin fold also observed in TFIIB. One hundred and thirty-two out of the 217 C alpha atoms from the cyclin fold can be superposed with a root-mean-square difference of 1.8 A. The structural homology is even higher for the residues at the interface with the kinase, which is of functional significance, as shown by our observation that cyclin H binds to cyclin-dependent kinase 2 (cdk2) and that cyclin A is able to activate cdk7 in the presence of MAT1. Based on this superposition, a new signature sequence for cyclins was found. The specificity of the cyclin H molecule is provided mainly by two long helices which extend the cyclin fold at its N- and C-termini and pack together against the first repeat on the side opposite to the kinase. Deletion mutants show that the terminal helices are required for a functionally active cyclin H.     

Plasminogen activation in synovial tissues: differences between normal, osteoarthritis, and rheumatoid arthritis joints

OBJECTIVE: To analyse the functional activity of the plasminogen activators urokinase (uPA) and tissue type plasminogen activator (tPA) in human synovial membrane, and to compare the pattern of expression between normal, osteoarthritic, and rheumatoid synovium. The molecular mechanisms underlying differences in PA activities between normal and pathological synovial tissues have been further examined. METHODS: Synovial membranes from seven normal (N) subjects, 14 osteoarthritis (OA), and 10 rheumatoid arthritis (RA) patients were analysed for plasminogen activator activity by conventional zymography and in situ zymography on tissue sections. The tissue distribution of uPA, tPA, uPA receptor (uPAR), and plasminogen activator inhibitor type-1 (PAI-1) was studied by immunohistochemistry. uPA, tPA, uPAR, and PAI-1 mRNA values and mRNA distribution were assessed by northern blot and in situ hybridisations respectively. RESULTS: All normal and most OA synovial tissues expressed predominantly tPA catalysed proteolytic activity mainly associated to the synovial vasculature. In some OA, tPA activity was expressed together with variable amounts of uPA mediated activity. By contrast, most RA synovial tissues exhibited considerably increased uPA activity over the proliferative lining areas, while tPA activity was reduced when compared with N and OA synovial tissues. This increase in uPA activity was associated with increased levels of uPA antigen and its corresponding mRNA, which were localised over the synovial proliferative lining areas. In addition, in RA tissues, expression of the specific uPA receptor (uPAR) and of the plasminogen activator inhibitor-type 1     

The USC CreativeIT database of multimodal dyadic interactions: from speech and full body motion capture to continuous emotional annotations

Improvised acting is a viable technique to study expressive human communication and to shed light into actors’ creativity. The USC CreativeIT database provides a novel, freely-available multimodal resource for the study of theatrical improvisation and rich expressive human behavior (speech and body language) in dyadic interactions. The theoretical design of the database is based on the well-established improvisation technique of Active Analysis in order to provide naturally induced affective and expressive, goal-driven interactions. This database contains dyadic theatrical improvisations performed by 16 actors, providing detailed full body motion capture data and audio data of each participant in an interaction. The carefully engineered data collection, the improvisation design to elicit natural emotions and expressive speech and body language, as well as the well-developed annotation processes provide a gateway to study and model various aspects of theatrical performance, expressive behaviors and human communication and interaction.     

Glass transition temperatures and some physical and sensory changes in stored spray-dried encapsulated flavors

Commercial spray-dried powder flavors (strawberry and orange) encapsulated in different amorphous matrices (maltodextrin and maltodextrin-sucrose) were stored for 20 days under constant relative humidities of 32%, 43%, 58% and 75%. Glass transition temperatures (T_g) of the different powders and maltodextrin DE 12 were measured using differential scanning calorimetry (DSC). Caking/collapse visual observations and aroma strength (measured by a trained sensory panel) were recorded and correlated with the glass transition temperature of the different spary-dried flavors. Glass transition data explained collapse occurrence and loss of aroma strength in encapsulated strawberry and orange flavors after storage at various relative humidities.The presence of sucrose in the carrier formulation negatively affects storage stability of the encapsulated flavor.     

A real-time protocol for the Internet based on the least mean square algorithm

Generally, real-time applications based on the User Datagram Protocol (UDP) generate large volumes of data and are not sensitive to network congestion. In contrast, Transmission Control Protocol (TCP) traffic is considered "well-behaved" because it prevents the network becoming congested by means of closed-loop control of packet-loss and round-trip-time. The integration of both sorts of traffic is a complex problem, and depends on solutions such as admission control that have not yet been deployed on the Internet. Moreover, the problem of quality-of-service (QoS) and resource allocation is extremely relevant from the point of view of convergence of streaming media and data transmission on the Internet. In this paper an adaptive real-time protocol based on the least mean square (LMS) algorithm is proposed to estimate the application UDP bandwidth in order to reduce the quadratic error between the packet loss and a target. Moreover, the LMS algorithm is also applied to make sure that the reduction in the average bandwidth allocated to each TCP process will not be higher than a given percentage of the average bandwidth allocated before the beginning of the UDP application.     

A mechanistic approach to extract the mechanical properties of individual constituents in plasma-sprayed metal matrix composite coatings

A mechanistic approach to determine the in-situ properties of individual constituents in a plasma sprayed metal matrix composite (MMC) coating was proposed. The approach was based on micro-indentation and inverse analysis techniques. Utilising the indentation data obtained from the micro-indentation experiments, elastic moduli of each constituent were calculated using a well-established method whereas yield strength and hardening exponent were extracted using the inverse procedure based on finite element analysis. Finite element results gave a satisfactory agreement between the numerically simulated and the measured indentation load-depth curves. Further studies using three dimensional finite element analyses of Vickers indentation on the MMC coating based on its actual microstructure also showed that the indentation behaviour of the MMC coatings is strongly dependent on its morphology, volume fraction, size and distribution of the reinforcing phase.     

Seed weight variation of Wyoming sagebrush in northern Nevada.

Seed size is a crucial plant trait that may potentially affect not only immediate seedling success but also the subsequent generation. We examined variation in seed weight of Wyoming sagebrush (Artemisia tridentata ssp. wyomingensis Beetle and Young), an excellent candidate species for rangeland restoration. The working hypothesis was that a major fraction of spatial and temporal variability in seed size (weight) of Wyoming sagebrush could be explained by variations in mean monthly temperatures and precipitation. Seed collection was conducted at Battle Mountain and Eden Valley sites in northern Nevada, USA, during November of 2002 and 2003. Frequency distributions of seed weight varied from leptokurtic to platykurtic, and from symmetry to skewness to the right for both sites and years. Mean seed weight varied by a factor of 1.4 between locations and years. Mean seed weight was greater (P < 0.05) in 2003 than in 2002 at both sites. This can partially be attributed to 55% greater precipitation in 2003 than 2002, since mean monthly temperatures were similar (P > 0.05) in all study situations. Simple linear regression showed that monthly precipitation (March to November) explained 85% of the total variation in mean seed weight (P = 0.079). Since the relationship between mean monthly temperature (June-November) and mean seed weight was not significant (r2 = 0.00, P = 0.431), this emphasizes the importance of precipitation as an important determinant of mean seed weight. Our results suggest that the precipitation regime to which the mother plant is exposed can have a significant effect on sizes of seeds produced. Hence, seasonal changes in water availability would tend to alter size distributions of produced offspring.