Sexual dimorphism of circulating somatotropin, insulin-like growth factor I and II, insulin-like growth factor binding proteins, and insulin: relationships to growth rate and carcass characteristics in growing lambs

The effects of sex and age on patterns of circulating somatotropin (ST) concentration and plasma IGF-I, IGF-II, insulin, and IGF binding protein-3 (IGFBP-3) were studied in ram, wether, and ewe lambs (n = 7 or 8) sampled at mean ages of 81 (I1) and 158 d (I2). Between 81 and 158 d of age, rams grew more rapidly than wethers (P < .01), and wethers grew more rapidly than ewes (P < .01). The sex differences in growth were reflected in empty body weight at slaughter: rams > wethers > ewes (P < .05). Mean plasma ST concentrations, ST pulse amplitude, and integrated plasma ST concentrations were greater (P < .05) in rams than in ewes at I1 and I2. Characteristics of the ST plasma profile in wethers were generally intermediate between those of rams and ewes. The interpulse interval was greater in ewes than in wethers at I2. The IGF-I and IGFBP-3 concentrations were greater in rams than in ewes at both sampling times. Plasma IGF-II was greater in ewes than in rams at I2. Mean plasma ST was approximately two thirds less at I2 than at I1 regardless of sex. Mean plasma ST and IGF-I at both ages were positively correlated with growth. Mean plasma ST at I2 was negatively correlated with fatness at slaughter. Sex and age significantly affected patterns of circulating ST and concentrations of IGF-I and IGFBP-3 in prepubertal growing lambs, under conditions for which growth rates and composition were also sexually dimorphic.     

Envelope glycoprotein gp120 of human immunodeficiency virus type 1 alters ion transport in astrocytes: implications for AIDS dementia complex

Infection by human immunodeficiency virus type 1 (HIV-1) is often complicated by a variety of neurological abnormalities. The most common clinical syndrome, termed acquired immunodeficiency syndrome (AIDS) dementia complex, presents as a subcortical dementia with cognitive, motor, and behavioral disturbances and is unique to HIV-1 infection. The pathogenesis of this syndrome is poorly understood but is believed to involve interactions among virally infected macrophages/microglia, astrocytes, and neurons. In this study, we show that exposure of primary rat and human astrocytes to heat-activated HIV-1 virions, or to eukaryotically expressed HIV-1 and HIV-2 envelope glycoproteins (gp120) stimulates amiloride-sensitive Na+/H+ antiport, potassium conductance, and glutamate efflux. These effects are blocked specifically by amiloride, an inhibitor of Na+/H+ antiport and by the selective removal of gp120 with immobilized monoclonal antibody. As a result of modulation of astrocytic function by gp120, the ensuing neuronal depolarization and glutamate exposure could activate both voltage-gated and N-methyl-D-aspartate-regulated Ca2+ channels, leading to increases in intraneuronal Ca2+ and neuronal death. These findings implicate the astrocyte directly in the pathogenesis of AIDS dementia complex.     

Validation of an inhibition ELISA using a monoclonal antibody for foot-and-mouth disease (FMD) primary diagnosis

An inhibition ELISA (IH-ELISA) test for foot-and-mouth disease virus (FMDV) was validated using 106 epithelial samples from suspected cases of FMD in Argentina submitted to the Argentine National Diagnostics Laboratory (GELAB) over a period of 12 months and examined in parallel with the complement fixation test (CFT). IH-ELISA was found to be more sensitive, detecting 25% (26 samples) more FMDV positives than the CFT in original suspensions of field samples. The effect of storage conditions on 12S stability was examined. Plates stored at 4 degrees C blocked with 1% ovalbumin and plates stored at -20 degrees C with or without blocking buffer could be used for at least 90 days. When various brands of polystyrene plates were compared for 12ps adsorption it was found that those microplates of higher binding capacity were more efficient.     

Oral health assessment in dogs: study design and results

The authors have conducted a large number of canine dental studies during the past six years. Study methodologies have been driven by the need for statistical validation of results, requiring the participation of large numbers of animals in each study. For plaque, stain, and calculus evaluations, a "clean tooth" model was used, in which formation of these substrates was assessed days to weeks after an initial coronal cleaning. A primary goal of the studies described was to validate the study designs used--that is, to use accepted plaque-, calculus-, and stain-controlling agents to test whether the study methods could detect differences between treated and untreated groups. The results of clinical studies show that the methods described can be used to identify plaque, calculus, and stain control attributable to chemical agents, oral hygiene aids, and consumable products.     

Diagnostic value of the reaction of morphological dissociation of clotted blood (RMDCB) in nonspecific pneumonias and lung cancer

The authors studied separate parameters of the reaction of morphological dissociation of clotted blood in 97 patients, 83 of them suffering chronic nonspecific pneumonia and 14--lung cancer. Such RMDCB tests as the amount of the third fraction of blood coagulation (TFCB), the time of TFCB sediment formation and "the prognostic index" are found to be of certain diagnostic value, and along with other laboratory tests and clinical symptoms these allowed a differentiation between chronic nonspecific pneumonia and lung cancer.     

基于高海拔地区的大气中子单粒子效应实时测量试验研究

开展65 nm高速大容量静态随机存取存储器（SRAM）大气中子单粒子效应特性及试验评价技术研究,基于4 300 m高海拔地区大面积器件阵列实时测量试验,突破效应甄别、智能远程测控等关键技术,在153 d的试验时间内共观测到错误43次,其中器件内单粒子翻转39次,多单元翻转（MCU）在单粒子翻转中占比23%,最大的MCU为9位。对高能中子、热中子和封装α粒子的贡献比例进行了分析,并基于多地中子通量数据,推演得到北京地面和10 km高空应用时的单位翻转（SBU）和MCU失效率（FIT）。发现地面处软错误的主要诱因为封装α粒子,随着海拔的增高,大气中子对软错误的贡献比例明显增大;MCU全部由高能中子引起,北京10 km高空处的MCU FIT值明显增大,其占比由地面的8%增大至26%。结合器件版图布局,对MCU产生机理进行了深入分析。最后,提出一种目标导向的存储器软错误加固策略优化方法。     

高加速应力试验及极限应力试验综述

介绍了高加速应力试验(HAST)及极限应力试验(limit test)的基本概念和相关背景,列举了当前国内外一些常用的技术方法、工程应用状况、试验模型及分析方法、试验剖面的建立及失效机理的确定等。指出了高加速应力试验及极限应力试验要注意的问题,并对它们的发展动向作了简单的预测。