The heterogeneity of cell populations by the cytochemical characteristics of the cellular chromatin

Cell heterogeneity of cytochemical characteristics of chromatin in the norm and after provocative factors in vitro (a heating at 45 degrees C, X-irradiation and joint actions of heating and X-ray irradiation) was investigated in the peripheral blood mononuclear cells of sheep and mouse, the lymphoid cell populations of the murine bone marrow, spleen and thymus. As a criterion of cytochemical heterogeneity were used the distinctions in the rate of staining of cell nuclei with DNA-specific dyes, that was registered by means of measurement of coefficient variation of the DNA-histogram basis peak, with a flow cytometry. Heterogeneity of cytochemical characteristics are dealt with showed a variability of spatial organization of interphase nuclei of single cells. The provocative factors bring about a decrease in the level of the cytochemical characteristics heterogeneity in the sheep mononuclear cells. In the murine cell populations studied no such equalizing of the marker of variability was revealed.     

The role of the extracorporeal detoxication in prophylaxis and treatment of respiratory disorders in patients with acute ileus

Hemosorption (HS) and plasmapheresis (PA) stimulate significantly the pulmonary metabolic functions due to their potent detoxicational effect. Arterial hypoxemia occurs simultaneously. The PA application contra HS causes more durable and stable detoxicational effect. It is mandatory in patients with an acute ileus to combine the HS conduction and repeated sessions of spontaneous respiration with end-expiratory additional pressure and to include fatty emulsions in complex of substitutional therapy while PA application.     

Analysis of the dynamics of transitions in Leptomonas collosoma spliced leader RNA and a possible role of gaps upon splicing

In [1], the existence of two competing secondary structures at the 5'-end of Leptomonas collosoma spliced leader RNA was shown. On going from one steady-state secondary structure to the other, and vice versa, RNA passes through several states in which the chain gets tied up into nodes. If the transitions are reversible, the nodes are figurative both in the forth and back directions although the secondary structures that arise in this process are quite different, and the forth and back routs do not coincide. It is assumed that under certain conditions, the chain can get tied into the next node of series 5(2), which most likely will not get untied. This node is tightened around GG nucleotides. In this case, the migration of the gap is possible, as a result of which the chain can break. Independent experiments indicate that the RNA chain breaks just at this site upon splicing. Based on these results, a possible role of nodes in the enzymatic activity is discussed.     

Effects of emoxipine and histochrome on lipid peroxidation and activity of serum MB-creatine phosphokinase in patients with ischemic heart disease during aortocoronary shunting]

Comparative study of the cardioprotective effect of antioxidants emoxipin and hystochrom was conducted in patients with chronic ICD during and after operation for aorto-coronary shunting. Both drugs effectively inhibited LPO activation and reduced the reperfusion damage to the myocardium recorded according to the release of MB-PCK into the blood. The new antioxidant hystochrom proved to be more effective. Its prevalent effect is associated with its higher antioxidant activity.     

Prophylaxis of postop bronchopulmonary complications during surgical treatment of inflammatory diseases of hepatobiliary tract

The combination of own breathing with heightened end-expiratory pressure and antibiotic prophylaxis, ultrasonic inhalation using proteolytic enzymes, bronchofibroscopic sanation have permitted to reduce the occurrence frequency of pulmonary complications from 16 to 7%.     

The characteristics of the spinal trauma in catapulted pilots

The article discusses the peculiarities of spinal fractures in catapulted pilots: mechanism of fracture--indirect axial load in combination with front or front-lateral bending; morphological picture--wedge-shaped compression fracture of vertebral body; type of fracture--stable; clinic peculiarities--scanty clinic picture as a rule; localization--mid-thorax section and thoracolumbar zone; number of vertebrae affected--1 as a rule. Diagnostic and treatment recommendations are enclosed. The author also deals with a phenomenon which accompanies the emergency abandon of an aircraft--microlesion of spongy osseous tissue of vertebrae without fracture signs under the influence of shock overload, which later can be the reason of degenerative and dystrophic diseases of spine. Preventive and medical measures are described which assure an adequate rehabilitation of functional state of spine.