An experimental study of the possibilities of using pentoxifylline for the prevention of cancer at different sites

Three models of cancerogenesis were used to test the anti-cancerogenic effects of pentoxiphylline. In female rats, breast adenocarcinoma was induced by intramammary injections of N-methyl-N-nitrosourea (MNU) or colonic and rectal adenocarcinomas by intrarectal instillations of MNU. In female mice, squamous cell carcinomas of the cervix uteri and vagina were induced by intravaginal applications of 7,12-dimethyl-benz(a) anthracene (DMBA). Pentoxifylline was given with drinking water at a concentration of 500 mg/l long at the stage of carcinogenesis promotion/progression. Pentoxifylline exerted a strong inhibitory effect on the development of mammary tumors and a moderate inhibitory effect on the development of colonic and rectal tumors induced by MNU in rats. However, the drug did not affect the development of cervical and vaginal tumors caused by DMBA in mice.     

Free-radical oxidation, the glutathione system and the status of the gastric mucosa in peptic ulcer in adolescents and young people under dynamic restorative treatment

1.5-2 versus 3-4 months following the in-patient stage of treatment without further antiradical therapy, patients-juveniles and young people with ulcer disease demonstrate compensated versus decompensated disorders in the glutathione system activity respectively, which results in disturbances in the free-radical oxidation processes, amplification of alteration, decrease in proliferation in the mucous membrane of the stomach. Antiradical therapy being continued avoids such a pathology. A necessity is substantiated for taking measures on an uninterrupted long-term basis, aimed to correct the free-radical oxidation processes in patients with peptic ulcer of juvenile age and young people.     

The pathogenetic basis for detoxifying and antioxidant therapy in the combined treatment of chronic diffuse liver diseases

As many as 98 patients with chronic diffusive liver diseases were studied for markers of endogenous intoxication syndrome as they were for intensity of lipid peroxidation (LPO) processes, condition of the antiradical defence systems, and detoxicating effectiveness of the drug "Thiotriasoline". Revealed in the patients were intensification of LPO processes, enhancement of the glutathionic system enzymes activity, decrease in superoxide dismutase. Accumulation in blood of malonic dialdehyde and molecular LPO products is believed to be a factor of the endogenous intoxication syndrome progression. A correlation was found between activity of LPO processes, intensification of endotoxicosis and degree of activity of the inflammatory process in the liver. The results obtained attest to the need for the antioxidant and adequate detoxicative therapy. High detoxicating efficacy of the drug "Thiotriasoline" has been shown.     

Significance of adrenal hormones in brain energy metabolism

Intensity of glycolysis was studied under conditions of immobilization stress and adrenalectomy as well as with administration of adrenaline and hydrocortisone. The processes of aerobic glycolysis, activity of enzymes of the tricarboxylic acid cycle and mitochondria respiratory chain, content of total, free and protein-bound glycogen, glucose-I-phosphate, glucose-6-phosphate, pyruvate, alpha-ketoglutarate, cytrate and macroergs in the brain are shown to depend on the level of adrenal hormones producing a multilateral effect on a series of energy metabolism links.     

The anatomy, surgical technic, preservation and reperfusion of the rat liver in an experiment]

Preservation of hepar of 86 rats using Euro-Collins' and Bretschneider preservation solutions was realized in experiment. The animals hepar anatomy was studied thoroughly, the hepar preservation pattern was improved, the modified pattern of the hepar isolated perfusion was used. Morphologic, cytologic and biochemical investigations were conducted to estimate the viability of hepar.     

Serological properties of HIV-1 isolates from the focus of an epidemic in the Gomel region of the Belarus Republic (1996)

Immunoreactivity of sera obtained in 1996 from HIV-1-infected intravenous narcomaniacs from an epidemic focus in Gomel region, Belarus, is studied with a panel of 10-16-component synthetic peptides simulating apical epitope of surface glycoprotein gp120 V3 loop of HIV-1 variants. Comparative analysis of resultant spectra with representative immunoreactivity spectra of sera from a sampling of HIV-1-positive sera collected in 1986-1997 in different regions of the former USSR demonstrated a high homogeneity of immunoreactivity spectra of sera from Gomel region. Serotypes of HIV-1 A/C variants circulating in populations of intravenous narcomaniacs in the focus in Gomel region in 1996 and in Southern and South-Eastern Ukraine in 1995-1996 are similar. This confirms a previous conclusion about the prevalence of A/C serotype among intravenous narcomaniacs in the former USSR.     

Clinical course and treatment of herpetic trigeminal ganglionic neuropathy

45 patients were observed in the periods of both acute herpes zoster and postherpetic neuralgia (PHN). In most of the patients herpetic eruptions were located in the areas of innervation of the first branch of the trigeminal nerve. In acute period of the disease there were used aciclovir, helepin or alpisarinum, antiherpetic immunoglobulin, deoxyribonuclease, non-narcotic analgetics were used. Of 28 patients residual PHN was observed in 6 cases, delayed PHN (during 3 months)--in 2 patients. The PHN development was characteristic for elderly patients, delayed request for medical care, concomitant diseases, eruptions with hemorrhagic component and secondary pyodermia and considerable residual sensory deficit. In therapy of PHN the most effective drugs were amitriptylin, non-narcotic analgetics, anticonvulsants as well as acupuncture and electroacupuncture. Relief of a typical deafferentation of pain syndrome was achieved by means of ultrasonic destruction of the trigeminal nucleus (one case). Early therapy of acute herpes zoster does not prevent completely PHN development, but it decreased considerably probability of its forming as well as the severity of its course.     

Analysis of the immunologic heterogeneity of HIV-1: I. "Arginine-glutamine trigger" in the fourth position of the central tetrapeptide of the V3 loop of gp120

This article reviews the existing knowledge base concerning the biology of spinal fusion, with the understanding that the focus is weighted toward posterolateral lumbar spinal fusion because of a relative paucity of biologic information on healing of other types of fusions. The discussion focuses first on the basic science of spinal fusion healing from the standpoint of animal modeling. Next, the discussion centers on the multitude of local factors that can affect fusion healing. Finally, the numerous systemic factors known to affect fusion healing are discussed.