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We have isolated a labile, specific sodium pump inhibitor or digitalis-like factor from the peritoneal dialysate of volume-expanded renal failure patients whose levels correlated closely with volume status and blood pressure. This study characterizes the inhibitory profile of this agent compared with that of ouabain against the three alpha-isoforms of the sodium pump. We prepared microsomal Na,K-ATPase from rat tissues representing the highest proportion of one of the alpha-isoforms. Both Northern and Western blot analyses confirmed that kidney had predominantly the alpha1-isoform, skeletal muscle the alpha2-isoform, and fetal brain the alpha3-isoform. Ouabain (5 x 10(-6) mol/L) produced little inhibition of kidney Na,K-ATPase (3.4+/-2.0%) but significant inhibition of skeletal muscle (37.2+/-3.7%, P<.001) and fetal brain (38.8+/-3.5%, P<.001) activity. In contrast, the labile digitalis-like factor, causing comparable inhibition of fetal brain Na,K-ATPase activity (33.3+/-4.7%), produced markedly greater inhibition of kidney (42.5+/-5.6%, P<.001) and moderately greater inhibition of skeletal muscle pump activity (57.7+/-6.3%, P<.05). In addition, the labile digitalis-like factor produced a marked concentration-dependent inhibition of the alpha2- and alpha3-isoforms (r=.79, P=.00005). Experiments combining the labile digitalis-like factor and ouabain confirmed that digitalis-like factor, unlike ouabain, was an effective inhibitor of all three isoforms in rat, in particular alpha2. The different pattern of isoform sensitivity displayed by the labile digitalis-like factor and ouabain further differentiates the two agents and raises some interesting possibilities about the functional implications of the endogenous factor. 相似文献
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Stefania De Rosa Paolo Branchini Valentina Spampinato Alexis Franquet Rossella Yivlialin Lamberto Duo Gianlorenzo Bussetti Luca Tortora 《Nano Research》2022,(2):1120-1127
A detailed stratigraphic investigation of the intercalation mechanism when graphite electrodes are immersed inside diluted perchloric(HClO4)and sulfuric(H2SO4)electrolytes is obtained by comparing results when graphite crystals are simply immersed in the same acid solutions.By combining time-of-flight secondary ion mass spectrometry(ToF-SIMS)and in-situ atomic force microscopy(AFM),we provide a picture of the chemical species involved in the intercalation reaction.The depth intensity profile of the ion signals along the electrode crystal clearly shows a more complex mechanism for the intercalation process,where the local morphology of the basal plane plays a crucial role.Solvated anions are mostly located within the first tens of nanometers of graphite,but electrolytes also diffuse inside the buried layers for hundreds of nanometers,the latter process is also aided by the presence of mesoscopic crystal defects.Residual material from the electrolyte solution was found localized in well-defined circular spots,which represent preferential interaction areas.Interestingly,blister-like micro-structures similar to those observed on the highly oriented pyrolytic graphite(HOPG)surface were found in the buried layers,confirming the equivalence of the chemical condition on the graphite surface and in the underneath layers. 相似文献
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Botjan Brank Lamberto Briseghella Nicola Tonello Frano B. Damjani 《International journal for numerical methods in engineering》1998,42(3):409-442
Continuum and numerical formulations for non-linear dynamics of thin shells are presented in this work. An elastodynamic shell model is developed from the three-dimensional continuum by employing standard assumptions of the first-order shear-deformation theories. Motion of the shell-director is described by a singularity-free formulation based on the rotation vector. Temporal discretization is performed by an implicit, one-step, second-order accurate, time-integration scheme. In this work, an energy and momentum conserving algorithm, which exactly preserves the fundamental constants of the shell motion and guaranties unconditional algorithmic stability, is used. It may be regarded as a modification of the standard mid-point rule. Spatial discretization is based on the four-noded isoparametric element. Particular attention is devoted to the consistent linearization of the weak form of the initial boundary value problem discretized in time and space, in order to achieve a quadratic rate of asymptotic convergence typical for the Newton–Raphson based solution procedures. An unconditionally stable time finite element formulation suitable for the long-term dynamic computations of flexible shell-like structures, which may be undergoing large displacements, large rotations and large motions is therefore obtained. A set of numerical examples is presented to illustrate the present approach and the performance of the isoparametric four-noded shell finite element in conjunction with the implicit energy and momentum conserving time-integration algorithm. © 1998 John Wiley & Sons, Ltd. 相似文献
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The structures of two unusual fatty acids, the knownrans-3-hexadecenoic acid and a noveltrans-3-tetradecenoic acid, both isolated from the Nova Scotian sea-weedsPalmaria palmata andChondrus crispus, were positively identified. After the extraction of the total fatty acids by saponification, followed by methylation, the
monoenoictrans fractions were isolated by thin-layer chromatography on silica gel impregnated with silver nitrate. The monoenoictrans fractions were derivatized with 2-amino-2-methyl-propanol prior to analysis by gas chromatography/mass spectrometry. The
mass spectra showed prominent [M+1]+ ions but lacked the 12 amu interval useful for identifying the double bond position. Hence, alternative diagnostic peaks
were used to confirm the position of the double bond in these two fatty acids. As thetrans-3-hexadecenoic acid is found in the photosynthetic tissue of all plants, it may also be present in ruminant fats and, presumably,
in human adipose tissue. 相似文献
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The energy policy in Europe regarding buildings and the energy efficiency sector are regulated by two directives: 2002/91/CE - EPBD and 2006/32/CE - EEESD
[1] and [2].The CEN has elaborated a standard to revise all European normatives about building energy performance and HVAC plants. The “CEN Umbrella” CEN/TR 15615 [3] includes a new version of EN ISO 13790:2008 [4] and a new standard about heating plant, use and contributions of renewable energy sources. Furthermore, the CEN Standard provides a software version for technicians.In this paper we present a Round Robin Test between a selection of softwares applied to an Italian case study. The selected calculation methodologies, range from research packages to extra-simplified software. Furthermore, the Italian procedure described in UNI TS 11300 [5] has been analyzed and considered as the reference for all other calculation methodologies.The results of the Round Robin Test show the relationship between thoroughness of data input and energy evaluation accurancy. The more the input data is affected by uncertainty, the less precise is the energy efficiency calculation. On the other hand the energy performance of building evaluation accurancy depends on the aim of the simulation: the energy audit, the energy design or the energy labeling (certification). 相似文献
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Anna Chiara Manetta Melania Giammarco Lorella Di Giuseppe Isa Fusaro Alessandro Gramenzi Andrea Formigoni Giorgio Vignola Lamberto Lambertini 《Food chemistry》2009
The distribution of aflatoxin M1 (AFM1) has been investigated in samples of whey, curd and a typical hard and long maturing cheese like Grana Padano (ripened for twelve months), produced with naturally contaminated milk in a range of 30–98 ng AFM1/kg. AFM1 determinations were carried out on 25 samples of each product by reverse-phase HPLC and fluorescence detection with post-column derivatisation, after a preliminary C18-SPE clean-up. Experimental results show that, in comparison to milk, AFM1 concentration levels increased both in curd (3-fold) and in long maturing cheese (4.5-fold), while AFM1 occurrence in whey decreased by 40%. 相似文献