Fibrinolytic therapy with low-dose recombinant tissue plasminogen activator in retinal vein occlusion

In a retrospective study the outcome of patients treated by the Kapandji-Sauvé procedure was evaluated. In the period from 1989 to 1993, 18 patients were operated. The arthrodesis of the distal radioulnar joint was performed by a dorsoulnar incision using one screw. A 2 cm long segment of the ulna was resected. Indications for Kapandji-Sauvé procedures were in 12 cases deformities subsequent to wrist fractures, in three cases osteoarthrosis of the distal radioulnar joint, in two cases Madelung's deformity and in one case Kienb?ck's disease. The mean follow-up time was 5.1 years (range 3 to 7 years). There was one case of reossification, which required reoperation. No other complications were seen. An excellent result was obtained in seven patients, a good result in nine patients, one patient showed satisfactory and one poor results. Grip strength was normal in six cases, reduced up to 20% in seven cases and reduced up to 50% in five cases. 13 patients returned to their previous occupation. 12 patients showed a range of pronation-supination of more than 150 degrees. There was no correlation between X-ray findings and clinical outcome in patients.     

Factors Influencing Destruction of Triacetone Triperoxide (TATP)

Acid catalyzes the formation of triacetone triperoxide (TATP) from acetone and hydrogen peroxide, but acid also destroys TATP, and, under certain conditions, converts TATP to diacetone diperoxide (DADP). Addition of strong acids to TATP can cause an explosive reaction, while reaction with dilute acid reduces the decomposition rate so drastically that gentle destruction of TATP is impractical. However, combined use of dilute acid with slightly solvated TATP made gentle destruction of TATP feasible. Variables including acid type, concentration, solvent and ratios thereof have been explored, along with kinetics, in an attempt to provide a field‐safe technique for gently destroying this homemade primary explosive. The preferred method is moistening TATP with an alcoholic solution (aqueous methanol, ethanol, or iso‐propanol) followed by addition of 36 wt‐% hydrochloric acid. Preliminary experiments have shown the technique to be safe and effective for destruction of hexamethylene triperoxide diamine (HMTD), as well.     

New flow cytometric capabilities at the national flow cytometryresource

The authors provide a brief review of flow cytometry and a broad view of new flow cytometry technologies. A brief introduction to flow cytometry and cell sorting is provided along with a summary of current commercial capabilities. New developments designed to overcome current limitations are described in terms of capabilities and a characterization of their performance. New capabilities include Fourier transform flow cytometry, phase sensitive detection, digital data acquisition, data clustering algorithms, high speed sorting, and slit scanning     

Patient management in geriatric psychiatry by ambulatory care services. Results of an empirical analysis

This study was designed to provide information to which extend home-based nursing care services for the elderly take part in the care for older people with mental disorders. Also of interest was the involvement of clinical facilities and services of the geropsychiatric treatment system in the health care for the clients. A one day data collection in 29 nursing care services in two North Rhine-Westphalian regions could raise informations about 1,246 clients aged 60 years and over 1,522 persons (41.8%) had a mental disorder, diagnosed by a nursing and/or medical professional. 68% of the mental disordered clients had dementia or a demential disorder, 6% a functional psychosis, and 31% a neurotic, psychogenic disorder or substance abuse (small number of clients with two and more diagnoses). 82% of clients with mental disorders had one or more additional somatic disease(s). These diseases were mostly the cause for the involvement of home-based nursing care service. Barely 8% of clients with mental disorders were placed from clinical facilities and services of the geropsychiatric treatment system into the nursing care services. Beside the home-based care, only a 12% of mental disordered received clients outpatient psychiatric treatment. Systematic cooperation between the nursing care services and the system of (gero-) psychiatric treatment was a rare exception.     

Recombinant tissue plasminogen activator in cases with fibrin formation after cataract surgery: a prospective randomised multicentre study

AIMS: This study investigated the effect of tissue plasminogen activator (tPA) in patients with severe intracameral fibrin after extracapsular cataract extraction or phacoemulsification with posterior chamber intraocular lens implantation. METHODS: A randomised prospective multicentre study was carried out in 86 patients with intraocular fibrin formation 2-8 days after cataract surgery. While the first group (n = 41) received only anti-inflammatory drugs, a single anterior chamber injection of tPA (10 micrograms) as an additional treatment to the standard was given in the second group (n = 44). On days 1, 2, 14, and 90 after randomisation, the visual acuities, slit lamp findings, and intraocular pressures were documented in standardised protocols. Efficacy of treatment was judged by the rate of fibrinolysis (primary objective), the frequency of synechiae, and central capsular fibrosis (secondary objectives). RESULTS: The incidence and quantity of intraocular fibrin were significantly lower in the patients treated with tPA than in the control group (p < 0.05). The frequencies of synechiae were reduced by tPA injection. The capsule fibrosis noted after 3 months was significantly lower in the tPA group (p = 0.027). No ocular side effects were noted after the tPA injections. CONCLUSIONS: Lysis of postcataract fibrin formation is accelerated and increased by a single intracameral injection of 10 micrograms tPA in addition to standard anti-inflammatory treatment. The findings suggest that the tPA injection reduces posterior capsule fibrosis, which still has to be addressed in larger study populations and with a long term follow up.     

Why do adults with mucoviscidosis refuse a medically recommended course of intravenous antibiotic therapy?

OBJECTIVE: To better understand the needs of spouses who provide care to spinal cord injury (SCI) survivors, by comparing their self-perceptions and complaints with those of their partners with disabilities and with those of spouses who do not provide care. DESIGN: Survey, including demographics, health concerns questionnaire, and administration of the Center for Epidemiologic Studies Depression Scale (CES-D), the Perceived Stress Scale (PSS), the Life Satisfaction Index (LSI-Z), and the Quality of Life and Individual Needs Questionnaire. SETTING: Two British SCI treatment centers, serving a defined population-based catchment area. PARTICIPANTS: One hundred twenty-four spouses of a longitudinally followed sample of SCI survivors, all of whom had been injured 23 or more years when the study was conducted in 1993. OUTCOME MEASURES: Scores on the above standardized tests, and responses to survey questions. RESULTS: Spouses had more depressive affect (p < .001) than their partners with disabilities, as measured by the CES-D. On the PSS, they exhibited no significant differences. Compared with spouses who were not caregivers, the caregiving spouses reported more physical stress (p = .005), emotional stress (p = .011), burnout (p = .007), fatigue (p = .002), and anger and resentment (p = .029). On the CES-D, they had more symptoms of depressive affect (p = .004) and somatic depression (p = .005). CONCLUSIONS: Spouses of long-term SCI survivors who fulfill a caregiving role report more symptoms of stress and depression than their partners with disabilities and other spouses who are not caregivers.     

The social concomitants of competitive and impatient/aggressive components of the Type A behavior pattern in preschool children: Peer responses and teacher utterances in a naturalistic setting.

The Matthews Youth Test for Health described by K. A. Matthews and J. Angulo (see record 1980-24519-001) was used to classify 120 children from 9 preschool classrooms as high or low on behaviors associated with the Type A pattern. Peer responses to the children were gathered sociometrically, and utterances of classroom teachers were tape recorded in a naturalistic setting. Boys gave higher sociometric ratings to high-competitive girls, and girls gave higher ratings to low-competitive boys. Teacher verbal utterances addressed to high-competitive children were characterized by less challenging cognitive processes and lower affective quality. Teacher verbal utterances addressed to children high on impatience/aggression were characterized by lower affective quality, fewer opportunities for child autonomy, more social/behavioral instructions, and more physical expressions of affection. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA

Isolation of monocytes by plastic adherence alters cell morphology and function. In order to study the effects of cell isolation procedures and subsequent culture on monocyte function, we examined cytoplasmic calcium concentration changes (delta[Ca2+]in) in human monocytes isolated by either negative (magnetic bead) or positive (plastic adherence) selection then stimulated with formyl-Met-Leu-Phe (fMLP), either immediately after isolation, or after 48 h in culture. We have previously shown that fresh adherence-isolated monocytes respond to fMLP with small delta[Ca2+]in and oxidative burst responses, exhibiting larger responses following 48 h of incubation. We now demonstrate that fresh monocytes, prevented from adhering by negative selection, exhibit an even smaller fMLP-induced delta[Ca2+]in, which does not increase during 48 h in culture if cells are kept nonadherent, in Teflon. Calcium responses of adherent, fresh monocytes do not increase if cells are subsequently placed into suspension and maintained nonadherent, but increase if nonadherent cells are permitted to adhere to plastic. Furthermore, augmented fMLP-[Ca2+]in and oxidative burst responses in plastic-adherent cells are restricted to a CD14-high phenotype subpopulation. The CD14-high phenotype also describes a subpopulation of cells that responds to CD4 crosslinking with a rapid delta[Ca2+]in. Induction of a subpopulation of CD14-high expressing cells by adherence may explain in part maturation-induced response changes observed in macrophage but not in monocyte in vitro systems.