The role of glutathione in dopaminergic neuronal survival

An increased production of reactive oxygen species is thought to be critical to the pathogenesis of Parkinson's disease. At autopsy, patients with either presymptomatic or symptomatic Parkinson's disease have a decreased level of glutathione in the substantia nigra pars compacta. This change represents the earliest index of oxidative stress in Parkinson's disease discovered to this point. This study compares the sensitivity of dopaminergic and nondopaminergic neurons in dissociated mesencephalic cultures to the depletion of glutathione. We have found that dopaminergic neurons are more resistant to the toxicity of glutathione depletion than nondopaminergic neurons. The possibility that dopaminergic neurons have a higher baseline glutathione level than nondopaminergic neurons is suggested by measurements of levels of cellular glutathione in a parallel system of immortalized embryonic dopaminergic and nondopaminergic cell lines. We also examined the role of glutathione in 1-methyl-4-phenylpyridinium toxicity. Decreasing the glutathione level of dopaminergic neurons potentiates their susceptibility to 1-methyl-4-phenylpyridinium toxicity, although 1-methyl-4-phenylpyridinium does not deplete glutathione from primary mesencephalic cultures. Our data suggest that although a decreased glutathione content is not likely to be the sole cause of dopaminergic neuronal loss in Parkinson's disease, decreased glutathione content may act in conjunction with other factors such as 1-methyl-4-phenylpyridinium to cause the selective death of dopaminergic neurons.     

欧洲城市中的性别主流化运动

欧洲城市和区域理事会（CCRE）下属的妇女委员会于2004年在欧盟范围内进行了一次调查，寻找真正适于妇女生存与发展的城市。调查中没有发现理想的城市，但是发现许多值得效仿的城镇，其中很多城市都制定了性别政策。     

Hydrogenation method based on electrodeposited layers controlling the hydrogen desorption rate

An innovative hydrogenation method to investigate the hydrogen embrittlement of metals and alloys is hereby presented. The benefits of electroplating samples with copper and nickel prior to gaseous hydrogenation at mid-range temperatures are quantified. It is showed that these electrodeposited layers allow to control the hydrogen desorption rate occurring after hydrogenation, during the cooling of the hydrogenated specimen. The present study demonstrates the capability of the method to control the introduced total hydrogen concentration within a margin of 0.2 wt.ppm. The applicability of the described method to further investigations into hydrogen concentrations effects on hydrogen embrittlement of ferritic alloys by the means of mechanical tests is evaluated.     

Effectiveness of simultaneous independent realizations at low carrier-to-noise ratio to improve heterodyne Doppler lidar performance. I. Theory and numerical simulations

It is shown that the performance of heterodyne Doppler lidar (HDL) can be improved by (i) at least one good realization for every single shot or (ii) several simultaneous good realizations for accumulation. Until now, several simultaneous independent realizations at high carrier-to-noise ratio (CNR) have been considered. At low CNR, noise may have a detrimental effect on the accumulation techniques. We determine the chances of getting "heavy" speckles in HDL signals from many receiver-detector units on a single-shot basis and several good realizations on a single-shot basis, which is required for an effective accumulation. The use of multiple receiver-detector units at low CNR is worthwhile in contexts such as space lidar, where optimized treatment is at a premium. We conclude on the effectiveness of many receiver-detector units in parallel in order to achieve simultaneous independent realizations at low CNR to improve the performance of HDL on a statistical basis.     

Kinetics of receptor-mediated radiotoxicity of 16alpha-[125I]-iodostradiol-3,17beta

AIM: The radiocytotoxic effects in estrogen receptor (ER) containing MCF-7 cells of a mamma carcinoma were investigated following incubation with [125I]E ranging from 1 h to 24 h. METHODS: The receptor status of the cells was confirmed by immunohistochemical staining. The accumulation of [125I]E in MCF-7 cells was tested in the presence and absence of radioinert E and [127I]E and in ER-negative cells in comparison to ER-positive cells. The subcellular distribution was investigated in 0.25 M Saccharose by ultra centrifugation. The radiocytotoxicity was assessed in ER-positive and negative cells by a standard colony forming assay after incubating with [125I]E (1.85 kBq/ml-55.5 kBq/ml) for 1, 2, 4, 8, 12, and 24 h. RESULTS: A significant cytotoxicity was observed only when ER-rich MCF-7-cells were incubated with [125I]E alone. The maximal cytotoxic effect was a reduction of survival fraction to 20-25%. This was achieved at radioactivity concentrations > 37 kBq/ml. Maximal effect was seen after 8 h incubation, extension of incubation time did not further increase toxicity. CONCLUSION: The results suggest that the radioactivity was bound to ER. Through their nuclear localization radioestrogens tagged with radionuclides emitting very low energy electrons (Auger electrons) bear potential for therapy by ER-mediated deposition of lethal doses of ionizing radiation to single cells without affecting neighbouring cells. But, instead of 125I the shorter-living 123I shall be used for labelling because the deciding radiation effectes occur within the first 8 h.     

Utilisation de la clinoptilolite en potabilisation des eaux—elimination de l'ion NH4+Clinoptilolite in drinking water treatment for NH4+ removal

The objective of this study is to develop a technique to remove ammonium ion from water intended for potable purposes. An ion exchange method is used with a selective ion exchanger, a natural cation zeolite, clinoptilolite. Glass columns (Fig. 1) are used for laboratory experiments. These experiments show that the NH₄⁺ exchange capacity is very small compared to its total capacity 2.17 meq g^?1; its value depends essentially on the NH₄⁺ initial concentration and less on the Ca²⁺ concentration in the influent water. Figure 3 illustrates the practical exchange capacity relative to the initial concentration of ammonium ion for a soft water (Ca²⁺ = 35–50 mg l^?1). We were particularly interested in waters weak in ammonium ion concentration (NH₄⁺ = 1–3 mg l^?1). In this case and for ～1 and 2 mg l^?1 NH₄⁺ concentration in water, the practical capacity is only 0.06 and 0.108 meq g^?1 respectively. The leakage is smaller than the ECC limit (European Community Council) for drinking waters (NH₄⁺ ? 0.5 mg l^?1) and the treated volume of water to breakthrough, defined at 0.5 mg l^?1 of NH₄⁺, is ?720 BV (BV = bed volume) in both cases.In another way Fig. 6 shows that hard waters (due to Ca²⁺ ions) are more difficult to treat than soft waters. The practical capacity is smaller than before and the NH₄⁺-leakage is greater. To lessen NH₄⁺-leakage to less than 0.5 mg l^?1 for soft waters down-flow and up-flow, regeneration is used. Figure 7 shows that up-flow regeneration is more attractive than down-flow regeneration.Cycle reproducibility (Figs 4 and 5) shows that the regeneration conditions satisfied our requirements: in this case, the salt consumption is 180 eq of salt per eq of NH₄⁺ eliminated. This prompted us to try to reuse the regenerant (with NH₄⁺ ion). An increase of NH₄⁺-leakage is noticed in the presence of an NH₄⁺-residual in the regenerant. This increase is more significant with down-flow regeneration.After these laboratory experiments, we carried out a semi-industrial pilot-plant. Our objective was first to verify the laboratory results and secondly to study clinoptilolite behaviour relative to the time it was used. Two plexiglass columns comprise the pilot-plant shown in Fig. 9; soft water is used for these experiments. The first column is regenerated with fresh salt solution. The cycles obtained, considering their initial NH₄⁺-concentration, are reproduced in Fig. 10. For 2 mg l^?1 NH₄⁺ in the influent water, the leakage is about 0.2 mg l^?1 and the treated volume to breakthrough (0.5 mg l^?1 of NH₄⁺) is about 750 BV. The second column is regenerated with a recycled solution. The quality of the cycles decreases with the number of reuse of the regenerant as shown in Fig. 11. Nevertheless, it is interesting to note that after 3 reuses, the performance decrease is only 25% and the leakage, although it increases is smaller than 0.5 mg l^?1.Pilot results allowed us to propose a treatment of 30,000 m³ day^?1; the cost per cubic meter water treated, relative to NH₄⁺-removal, is about 0.165 FF (0.033 US $) for a plant and 0.77 FF (0.014 US $) for the same plant at the seaside. Using two serial columns decreased the cost by about 40–50%.     

Facial animation retargeting and control based on a human appearance space

Expressive facial animations are essential to enhance the realism and the credibility of virtual characters. Parameter‐based animation methods offer a precise control over facial configurations while performance‐based animation benefits from the naturalness of captured human motion. In this paper, we propose an animation system that gathers the advantages of both approaches. By analyzing a database of facial motion, we create the human appearance space. The appearance space provides a coherent and continuous parameterization of human facial movements, while encapsulating the coherence of real facial deformations. We present a method to optimally construct an analogous appearance face for a synthetic character. The link between both appearance spaces makes it possible to retarget facial animation on a synthetic face from a video source. Moreover, the topological characteristics of the appearance space allow us to detect the principal variation patterns of a face and automatically reorganize them on a low‐dimensional control space. The control space acts as an interactive user‐interface to manipulate the facial expressions of any synthetic face. This interface makes it simple and intuitive to generate still facial configurations for keyframe animation, as well as complete temporal sequences of facial movements. The resulting animations combine the flexibility of a parameter‐based system and the realism of real human motion. Copyright © 2010 John Wiley & Sons, Ltd.