A Monotone Iterative Technique for Nonlinear Fourth Order Elliptic Equations with Nonlocal Boundary Conditions

This paper proposes an accelerated iterative procedure for a nonlinear fourth order elliptic equation with nonlocal boundary conditions. First, an existence and uniqueness theorem is proved for the fourth order elliptic equation via the accelerated iterative procedure. To solve this problem numerically, a finite difference based numerical scheme is also developed in view of the main theorem. Theoretically, the monotone property as well as the convergence analysis are proved for both the continuous and discretized cases. The main result also supplements several algorithms for computing the solution of the fourth order elliptic integro-partial differential equation. The proposed scheme not only accelerates the scheme in the literature but also provides a greater flexibility in choosing the initial guess. The efficacy of the proposed scheme is demonstrated through a comparative numerical study with the recent literature. The numerical simulation confirms the theoretical claims too.     

Effect of manuring and frequency of cutting on the yield of leaf protein from some fodder grasses

A study to find out the effect of manuring and the frequency of cutting on the yield of leaf protein was conducted with four fodder grasses. The study revealed that Cenchrus glaucus was superior to the other grasses tried and it gave the maximum yield of dry matter and extractable protein. Farm yard manure at 10 tons/ha along with ammonium sulphate at 33 kg N/ha proved to be the best in giving the highest yield of dry matter and leaf protein. For all the grasses the maximum yield was found to range within the first three cuttings.     

Determination of oxygen-to-metal ratios in metal oxide nuclear fuels

Methods for the determination of O/M ratios in uranium and uranium-plutonium oxide nuclear fuels are reported based on an extensive literature survey. The salient features, as well as the merits and drawbacks of each method are critically reviewed. The need for a fast direct method, by which the simpler indirect methods may be checked, is met by the carbon-reduction method.     

Low Pressure DC Glow Discharge Air Plasma Surface Treatment of Polyethylene (PE) Film for Improvement of Adhesive Properties

The present work deals with the change in surface properties of polyethylene (PE) film using DC low pressure glow discharge air plasma and makes it useful for technical applications. The change in hydrophilicity of the modified PE film surface was investigated by measuring contact angle and surface energy as a function of exposure time. Changes in the morphological and chemical composition of PE films were analyzed by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The improvement in adhesion was studied by measuring T-peel and lap-shear strength. The results show that the wettability and surface energy of the PE film has been improved due to the introduction of oxygen-containing polar groups and an increase in surface roughness. The XPS result clearly shows the increase in concentration of oxygen content and the formation of polar groups on the polymer surface. The AFM observation on PE film shows that the roughness of the surface increased due to plasma treatment. The above morphological and chemical changes enhanced the adhesive properties of the PE film surfaces, which was confirmed by T-peel and lap-shear tests.     

Experimental analysis of a two‐axis tracking system for solar parabolic dish collector

In this paper, an attempt has been made to develop a two‐axis tracking system for solar parabolic dish concentrator and experimentally evaluated the performance of the tracking system. In this proposed design, the sensor design uses the illumination produced by the convex lens on the apex of a pyramid to align the dish in‐line with the sun. The change in incident angle of the solar rays on the lens surface shifts the area of illumination from the apex of the pyramid towards its faces. Photodiodes placed on the faces of the pyramid are used as the sensitive elements to detect the movement of the sun. The sensor output is fed to a microcontroller‐based system to drive the stepper motor on the basis of the programmed algorithm such that it receives normal incidence of sunlight on the sensor. To evaluate the performance of the proposed system, a conventional available 1‐W photovoltaic (PV) panel is placed at the focal point to measure the short circuit current and open circuit voltage. With respect to the conventional solar PV panel, it is observed that the positioning accuracy of the proposed tracking system enhances the short circuit current of 0.11 A by 86%. Thus, the proposed tracking system can be used in a stand‐alone parabolic dish with concentrating PV module as the focal point for further studies.     

Potential of Pedilanthus tithymaloides as a petro-crop

Trials have been undertaken to study the comparative biomass and hydrocarbon production of seven different types of the plant Pedilanthus. After three years of growth the plants were cut and the resulting material subjected to acetone and benzene extraction. On the basis of the results obtained Pedilanthus tithymaloides var. cuculatus, P. tithymaloides var. variegatus I and P. tithymaloides (proper) are the most promising varieties for development as petro-crops, with potential for good biomass and total hydrocarbon yields.     

Role of GDF15/MAPK14 Axis in Chondrocyte Senescence as a Novel Senomorphic Agent in Osteoarthritis

Osteoarthritis (OA) is most prevalent in older individuals and exerts a heavy social and economic burden. However, an effective and noninvasive approach to OA treatment is currently not available. Chondrocyte senescence has recently been proposed as a key pathogenic mechanism in the etiology of OA. Furthermore, senescent chondrocytes (SnCCs) can release various proinflammatory cytokines, proteolytic enzymes, and other substances known as the senescence-associated secretory phenotype (SASP), allowing them to connect with surrounding cells and induce senesce. Studies have shown that the pharmacological elimination of SnCCs slows the progression of OA and promotes regeneration. Growth differentiation factor 15 (GDF15), a member of the tumor growth factor (TGF) superfamily, has recently been identified as a possible aging biomarker and has been linked to a variety of clinical conditions, including coronary artery disease, diabetes, and multiple cancer types. Thus, we obtained data from a publicly available single-cell sequencing RNA database and observed that GDF15, a critical protein in cellular senescence, is highly expressed in early OA. In addition, GDF15 is implicated in the senescence and modulation of MAPK14 in OA. Tissue and synovial fluid samples obtained from OA patients showed overexpression of GDF15. Next, we treated C20A4 cell lines with interleukin (IL)-1β with or without shGDF15 then removed the conditioned medium, and cultured C20A4 and HUVEC cell lines with the aforementioned media. We observed that C20A4 cells treated with IL-1β exhibited increased GDF15 secretion and that chondrocytes cultured with media derived from IL-1β–treated C20A4 exhibited senescence. HUVEC cell migration and tube formation were enhanced after culturing with IL-1β-treated chondrocyte media; however, decreased HUVEC cell migration and tube formation were noted in HUVEC cells cultured with GDF15-loss media. We tested the potential of inhibiting GDF15 by using a GDF15 neutralizing antibody, GDF15-nAb. GDF15-nAb exerted a similar effect, resulting in the molecular silencing of GDF15 in vivo and in vitro. Our results reveal that GDF15 is a driver of SnCCs and can contribute to OA progression by inducing angiogenesis.     

Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells

Background: Lung cancer remains a leading cause of cancer-related death, with an annual global mortality rate of 18.4%. Despite advances in diagnostic and therapeutic technologies, non–small cell lung carcinoma (NSCLC) continues to be characterized by a poor prognosis. This may be associated with the enrichment of cancer stem cells (CSCs) and the development of chemoresistance—a double-edged challenge that continues to impede the improvement of long-term outcomes. Metabolic reprogramming is a new hallmark of cancer. Sterol regulatory element-binding proteins (SREBPs) play crucial regulatory roles in the synthesis and uptake of cholesterol, fatty acids, and phospholipids. Recent evidence has demonstrated that SREBP-1 is upregulated in several cancer types. However, its role in lung cancer remains unclear. Objective: This study investigated the role of SREBP-1 in NSCLC biology, progression, and therapeutic response and explored the therapeutic exploitability of SREBP-1 and SREBP-1-dependent oncometabolic signaling and miRNA epigenetic regulation. Methods: We analyzed SREBP-1 levels and biological functions in clinical samples and the human NSCLC cell lines H441 and A549 through shRNA-based knock down of SREBP function, cisplatin-resistant clone generation, immunohistochemical staining of clinical samples, and cell viability, sphere-formation, Western blot, and quantitative PCR assays. We conducted in-silico analysis of miRNA expression in NSCLC samples by using the Gene Expression Omnibus ({"type":"entrez-geo","attrs":{"text":"GSE102286","term_id":"102286"}}GSE102286) database. Results: We demonstrated that SREBP-1 and SCAP are highly expressed in NSCLC and are positively correlated with the aggressive phenotypes of NSCLC cells. In addition, downregulation of the expression of tumor-suppressing hsa-miR-497-5p, which predictively targets SREBP-1, was observed. We also demonstrated that SREBP-1/SCAP/FASN lipogenic signaling plays a key role in CSCs-like and chemoresistant NSCLC phenotypes, especially because the fatostatin or shRNA targeting of SREBP-1 significantly suppressed the viability, cisplatin resistance, and cancer stemness of NSCLC cells and because treatment induced the expression of hsa-miR-497. Conclusion: Targeting the SREBP-1/hsa-miR-497 signaling axis is a potentially effective anticancer therapeutic strategy for NSCLC.