Immunocytochemical evaluation of protein kinase C translocation to the inner nuclear matrix in 3T3 mouse fibroblasts after IGF-I treatment

The complex pathway which links the agonist-cell membrane receptor binding to the response at the genome level involves, among other elements, protein kinase C (PKC). Agonists acting at the cell membrane can affect an autonomous nuclear polyphosphoinositide signaling system inducing an activation of nuclear phosphoinositidase activity and a subsequent translocation of PKC to the nuclear region. The fine localization of PKC has been investigated by means of electron microscopy quantitative immunogold labeling in 3T3 mouse fibroblasts, mitogenically stimulated by IGF-I. The enzyme, which in untreated cells is present in the cytoplasm, except for the organelles, and in the nucleoplasm, after IGF-I treatment is reduced in the cytoplasm and almost doubled in the nucleus. The PKC isoform translocated to the nucleus is the alpha isozyme, which is found not only associated with the nuclear envelope but mainly with the interchromatin domains. By using in situ matrix preparations, PKC appears to be retained at the nuclear matrix level, both at the nuclear lamina and at the inner nuclear matrix, suggesting a direct involvement in the phosphorylation of nuclear proteins which are responsible for the regulation of DNA replication.     

Vasoactive intestinal peptide-induced prolactin release in hypothyroid patients

VIP is an established prolactin-releasing factor. VIP gene expression at the anterior pituitary level and the central nervous system is regulated by thyroid hormones. On the other hand, primary hypothyroidism leads in many cases to amenorrhea, galactorrhea and hyperprolactinemia. In this study we assessed prolactin responses to VIP (75 micrograms iv infusion over 12 min) in a group of six hypothyroid women (mean age +/- SE, 38.8 +/- 3.3 yr; serum TSH levels, mU/L, 116.3 +/- 23.9), before treatment and after normalization of thyroid hormone levels during thyroxine (T4) replacement therapy (100-150 micrograms/day over 12-16 weeks). Furthermore, we assessed if VIP infusion had any effects on serum GH levels in these patients. In hypothyroid women, VIP infusion increased serum prolactin concentrations with peak levels being attained at 15 min (28.8 +/- 3.4 micrograms/L). The Area Under the Curve (AUC) was 1921 +/- 103 micrograms/L/2h. PRL responses to VIP were unchanged after T4 therapy, both in terms of peak levels (28.7 +/- 2.2 micrograms/L, NS) and of AUC (2079 +/- 261 micrograms/L/2h, NS). Serum GH levels were unaffected by VIP administration. In conclusion our study shows that, in hypothyroid patients, restoration of normal thyroid hormone levels by thyroxine replacement therapy does not affect lactotroph responsiveness to VIP. Therefore, our data do not support the hypothesis that VIP might contribute to the hypothyroid-induced hyperprolactinemia seen in man.     

富水电厂机组在线监测及状态检修

介绍了在富水电厂实施状态检修管理体制的情况以及与之相关的在线监测技术。     

L-Expressnet: The Communication Subnetwork for the C-NET Project

L-Expressnet is the communication suxbnetwork for the CNET local area network project of the CNR, Italy. Based on a single bus topology, it utilizes a simple and efficient virtual-token access protocol which provides ordered and collision-free transmission. The paper discusses the needs which led to L-Expressnet, describes the protocol, proves its correct operation, and compares its throughput-delay performance with those of other typical bus Protocols. A major advantage of L-Expressnet is that its implementation may be based on Ethernet standard transceivers and network interfaces.     

辽东凸起渤海X油田物源新认识

为明确辽东凸起渤海X油田的物源方向,结合地震、岩心、岩石组分、重矿物资料开展物源方向精细研究.结合薄片鉴定以及X-7D、X-5、X-4井重矿物成分分析,石榴子石和磁铁矿的高比例和相似分布特征,明确了X油田为远源沉积的单一物源.利用地震反射波阻特征及组合关系分析,确立了X油田物源方向为南东向.通过残余厚度法恢复了X油田的古地貌,认为古地貌呈现3个构造低点向2个构造低点转化的格局,为沉积中心.综合多方面研究,认为渤海X油田为来自南东方向的单一物源,受古地貌控制分为2、3个分支朵叶体,为储层发育区,可以部署开发井.该成果能够较好地指导油田开发方案的制定,并为相似油田提供借鉴.     

基于负载转矩观测器的动态恒风量控制

当前不基于外部传感器的恒风量控制技术的控制性能有限.针对此问题,提出一种基于负载转矩观测器的动态恒风量控制技术.构建降阶龙贝格负载转矩观测器,实现风机负载转矩及风道参数的实时观测,并进一步计算风压与风量.将实时计算的风量作闭环PI控制,形成风量-转速-电流三环控制.实测结果表明,所提出方法的风量控制精度高,且无需外部传感器,可有效兼顾实现低成本与高控制性能.     

基于负载转矩观测器的动态恒风量控制

当前不基于外部传感器的恒风量控制技术的控制性能有限.针对此问题,提出一种基于负载转矩观测器的动态恒风量控制技术.构建降阶龙贝格负载转矩观测器,实现风机负载转矩及风道参数的实时观测,并进一步计算风压与风量.将实时计算的风量作闭环PI控制,形成风量-转速-电流三环控制.实测结果表明,所提出方法的风量控制精度高,且无需外部传感器,可有效兼顾实现低成本与高控制性能.     

基于奖励引导的六足机器人自主步态学习

为提高机器人自主学习能力,提出一种基于奖励引导的机器人自主步态学习算法.首先给出自主设计的机械式步态同步六足机器人的机械结构,其独特的多层钢堆叠腿部设计方案和特有的机械同步传动方案为机器人后续步态学习研究奠定良好基础;接下来提出一种基于奖励引导的机器人步态学习算法,该算法可以让机器人在未知环境中进行技能学习;最后,在MATLAB和ADAMS联合仿真平台上进行算法验证实验,实验结果显示以速度和高度分别为主评价指标会呈现出2种合理的收敛结果,且在该算法下六足机器人可以自主学习到翻越木块的高难度技能.     

台风灾害下配电系统受损评估研究

台风灾害会造成配电系统多个元件同时故障而导致大面积用户停电。为研究台风灾害对于配电系统的破坏影响,提出台风灾害下配电系统受损情况评估方法。为描述台风特征对元件故障概率的影响,基于台风影响历史数据,使用随机森林算法建立元件脆弱性曲线。在元件模型建立后,使用时序蒙特卡洛模拟法评估配电系统的实时和累计电量及用户损失。为提升评估效率,引入故障模式后果分析法,使用最小路法生成故障影响报表,更快地确定失电负荷节点。最后通过RBTS-BUS6测试系统和广州地区的实际系统进行算例分析,验证了所提算法的有效性。结果表明：台风对配电网的影响具有延时性,配电系统受损情况与元件维修时间和维修资源数量相关。