Unusual Two-Step Assembly of a Minimalistic Dipeptide-Based Functional Hypergelator

Self-assembled peptide hydrogels represent the realization of peptide nanotechnology into biomedical products. There is a continuous quest to identify the simplest building blocks and optimize their critical gelation concentration (CGC). Herein, a minimalistic, de novo dipeptide, Fmoc-Lys(Fmoc)-Asp, as an hydrogelator with the lowest CGC ever reported, almost fourfold lower as compared to that of a large hexadecapeptide previously described, is reported. The dipeptide self-assembles through an unusual and unprecedented two-step process as elucidated by solid-state NMR and molecular dynamics simulation. The hydrogel is cytocompatible and supports 2D/3D cell growth. Conductive composite gels composed of Fmoc-Lys(Fmoc)-Asp and a conductive polymer exhibit excellent DNA binding. Fmoc-Lys(Fmoc)-Asp exhibits the lowest CGC and highest mechanical properties when compared to a library of dipeptide analogues, thus validating the uniqueness of the molecular design which confers useful properties for various potential applications.     

Sort vs. hash revisited

Efficient algorithms for processing large volumes of data are very important both for relational and new object-oriented database systems. Many query-processing operations can be implemented using sort- or hash-based algorithms, e.g. intersections, joins, and duplicate elimination. In the early relational database systems, only sort-based algorithms were employed. In the last decade, hash-based algorithms have gained acceptance and popularity, and are often considered generally superior to sort-based algorithms such as merge-join. In this article, we compare the concepts behind sort- and hash-based query-processing algorithms and conclude that (1) many dualities exist between the two types of algorithms, (2) their costs differ mostly by percentages rather than by factors, (3) several special cases exist that favor one or the other choice, and (4) there is a strong reason why both hash- and sort-based algorithms should be available in a query-processing system. Our conclusions are supported by experiments performed using the Volcano query execution engine     

The Development of Color Categories in Two Languages: A Longitudinal Study.

This study unites investigations into the linguistic relativity of color categories with research on children's category acquisition. Naming, comprehension, and memory for colors were tracked in 2 populations over a 3-year period. Children from a seminomadic equatorial African culture, whose language contains 5 color terms, were compared with a group of English children. Despite differences in visual environment, language, and education, they showed similar patterns of term acquisition. Both groups acquired color vocabulary slowly and with great individual variation. Those knowing no color terms made recognition errors based on perceptual distance, and the influence of naming on memory increased with age. An initial perceptually driven color continuum appears to be progressively organized into sets appropriate to each culture and language. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Kinetic Basicities of Aqueous Binary Mixtures: A Surprisingly Good Linear Correlation with the Gas Phase Basicity of the Organic Cosolvent

A remarkably good correlation between the rate of proton transfer from excited protonated 1-aminopyrene and the gas phase basicities of the organic component in water: organic solvent binary mixtures has been found. We suggest that use of anilinium acids minimizes the effect of anion solvation and Coulomb interactions and enables the influence of the organic cosolvent on the proton transfer to be clearly observed. The model proposed is one in which the proton is always transferred to a water molecule and a concerted proton transfer to a water molecule solvated by the organic cosolvent also occurs. The cosolvent influence is then on this latter species via hydrogen bond interactions. The correlation holds in pure water but not in pure organic solvents where, clearly, the proton must be transferred to the organic molecule.     

School psychologists' acceptability of behavioral and traditional assessment procedures for externalizing problem behaviors.

A national survey of school psychologists' acceptability of psychological assessment techniques in the area of externalizing problem behaviors was examined. School psychologists' ratings of 2 procedures, Behavioral Assessment (BA) and Traditional Assessment (TA), were compared using an analog case study methodology. Using a random sample of 500 National Association of School Psychologists members from the 1991–1992 membership directory, 339 Ss completed the Assessment Rating Profile after reading a hypothetical case summary describing a student with suspected externalizing problem behaviors. Results indicated statistically significant differences between assessment methods and ratings of acceptability. BA procedures were found to be more acceptable than TA procedures. Implications related to the acceptability of psychological assessment techniques, as well as implications for school psychology practitioners are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A system for real-time measurements of current-density distribution in low-energy electron beams with large cross sections

A system for real-time measurements of the current-density distribution in electron beams ejected from large-area electron accelerators has been developed. The system, which is based on Faraday cups, a microprocessor, and a PC, is designed for measuring the parameters of the electron beams ejected from large-area accelerators—devices for ionizing electroionization lasers and technological accelerators—and ensures real-time monitoring of the current-density distribution over the electron-beam cross section with a relative measurement accuracy of at least ±2%.     

Accuracy of teacher judgments in predicting oral reading fluency.

The relationship between teachers' judgments versus actual performance on curriculum-based measures in reading was examined. A total of 30 regular education teachers were asked to predict the oral reading fluency score of students along with a rating scale of reading subskills. Correlations between teacher judgment measures and student performance found that teachers were accurate reporters of student performance levels in reading. However, some questions were raised whether teachers were accurate at predicting the actual level of student oral reading fluency when effect sizes were used to compare teacher judgment and actual student performance. Recommendations for continued research in understanding the parameters of the accuracy of teacher judgment are made. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

End labeling studies of fragmented DNA in the avian growth plate: evidence of apoptosis in terminally differentiated chondrocytes

The chondro-osseous junction has been the subject of considerable scrutiny, especially in terms of the fate and role of the terminally differentiated chondrocyte. Although it has been proposed that these cells change their phenotype and survive in the epiphysis, possibly as osteoblasts, evidence from a number of other studies suggests that chondrocytes may undergo apoptosis or programmed cell death. A useful test for programmed cell death is to end label DNA in cryosections using the commercial reagent ApopTag and detect antibody binding to fragmented DNA by epifluorescence; more direct assessments include examination of the nucleus for condensation of chromatin evaluating fragmentation through alkaline and pulsed field agarose gel electrophoresis of DNA, and measuring apoptosis by flow cytometry. We found that we could label cells in the proliferative and the hypertrophic region of the proximal tibial growth plate of the chick with ApopTag. Most of the chondrocytes in the hypertrophic region were labeled by the reagent; in contrast, few proliferative chondrocytes were stained by the end-labeling procedure. Both agarose and pulsed field electrophoresis were used to confirm that there was fragmentation of chondrocyte DNA. Alkaline gel electrophoresis indicated that there was more fragmentation of DNA from hypertrophic cells than from proliferative chondrocytes. Further evidence in support of apoptosis was provided by electron microscopic observation of cells in the hypertrophic region of the growth plate. We noted that many of the cells in this region of the growth plate appeared to be undergoing programmed cell death since their nuclei contained condensed chromatin. Finally, we used flow cytometry to analyze chondrocytes isolated from the proliferating and hypertrophic regions of the growth plate for apoptosis. Dual parameteric flow cytometric contour plots of Hoechst and 7-amino-actinomycin D fluorescence showed that abut 8% of cells in the plate were apoptotic. Most of these cells were in hypertrophic cartilage. In summary, the results of this investigation indicate that chondrocytes terminate their life history by apoptosis. While it is possible that the terminal labeling studies may overestimate the number of cells undergoing this event, the data lend credence to the view that cells are removed from the epiphysis through apoptosis. If this is the case, then chondrocytes probably enter the terminal phase of their life as fully functioning cells and genomic, and/or local environmental conditions provide termination signals that initiate events that lead to programmed cell death.