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1.
本文介绍了一种实用的数据库设计方法,它以实体分析法为理论基础,通过对我们日常使用的表进行聚集和归类,完成数据库的逻辑设计,所设计的数据库满足BNOF范式。同时该方法可操作性较强,本文还介绍了自行设计的ERCM数据库辅助设计工具。 相似文献
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Bakos E T Hegedüs Z Holló E Welker GE Tusnády GJ Zaman MJ Flens A Váradi B Sarkadi 《Canadian Metallurgical Quarterly》1996,271(21):12322-12326
The membrane topology of the human multidrug resistance-associated protein (MRP) was examined by flow cytometry phenotyping, immunoblotting, and limited proteolysis in drug-resistant human and baculovirus-infected insect cells, expressing either the glycosylated or the underglycosylated forms of this protein. Inhibition of N-linked glycosylation in human cells by tunicamycin did not inhibit the transport function or the antibody recognition of MRP, although its apparent molecular mass was reduced from 180 kDa to 150 kDa. Extracellular addition of trypsin or chymotrypsin had no effect either on the function or on the molecular mass of MRP, while in isolated membranes limited proteolysis produced three large membrane-bound fragments. These experiments and the alignment of the MRP sequence with the human cystic fibrosis transmembrane conductance regulator (CFTR) suggest that human MRP, similarly to CFTR, contains a tandem repeat of six transmembrane helices, each followed by a nucleotide binding domain, and that the C-terminal membrane-bound region is glycosylated. However, the N-terminal region of MRP contains an additional membrane-bound, glycosylated area with four or five transmembrane helices, which seems to be a characteristic feature of MRP-like ATP-binding cassette transporters. 相似文献
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By using perfusions and bolus administration, coupled with postembedding immunocytochemical procedures, we have identified the structures involved in the transport of derivatized orosomucoid (alpha1-acidic glycoprotein) across the continuous microvascular endothelium of the murine myocardium. Our findings indicate that: (i) monomeric orosomucoid binds to the luminal surface of the endothelium; (ii) it is restricted to caveolae during its transport across the endothelium; (iii) it is detected in the perivascular spaces at early time points (by 1 min) and in larger quantities at later time points (>5 min) from the beginning of its perfusion or its intravascular administration; (iv) no orosomucoid molecules are found in the intercellular junctions or at the abluminal exits of interendothelial spaces; and (v) the vesicular transport of orosomucoid is strongly inhibited by N-ethylmaleimide (>80%). Because, by size and shape, the orosomucoid qualifies as a preferential probe for the postulated small pore system, our results are discussed in relation to the pore theory of capillary permeability. 相似文献
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Given that fretting wear causes failure in steel wires, we carried out tangential fretting wear tests of steel wires on a self-made fretting wear test rig under contact loads of 9 and 29 N and fretting amplitudes ranging from 5 to 180 μm. We observed morphologies of fretted steel wire surfaces on an S-3000N scanning electron microscope in order to analyze fretting wear mecha-nisms. The results show that the fretting regime of steel wires transforms from partial slip regime into mixed fretting regime and gross slip regime with an increase in fretting amplitudes under a given contact load. In partial slip regime, the friction coefficient has a relatively low value. Four stages can be defined in mixed fretting and gross slip regimes. The fretting wear of steel wires in-creases obviously with increases in fretting amplitudes. Fretting scars present a typical morphology of annularity, showing slight damage in partial slip regime. However, wear clearly increases in mixed fretting regime where wear mechanism is a combination of plastic deformation, abrasive wear and oxidative wear. In gross slip regime, more severe degradation is present than in the other regimes. The main fretting wear mechanisms of steel wires are abrasive wear, surface fatigue and friction oxidation. 相似文献
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To eliminate the perturbation of interceptor detection induced by aerodynamic heating,the head pursuit (HP) guidance law for three-dimensional interception was presented. The guidance law positioned the interceptor ahead of the target on its flight trajectory,and the speed of interceptor was required to be lower than that of the target. On the basis of a novel head pursuit three-dimensional guidance model,a nonlinear guidance law was developed based on smooth sliding mode control theory. At the same time,a special observer was designed to estimate the target acceleration,and a numerical example on maneuvering ballistic target interception verified the effectiveness of the presented guidance law. 相似文献
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A new preamble structure is designed for wireless LAN based on MIMO OFDM systems, which can be used for both synchronization and channel estimation. Modulatable orthogonal polyphase sequence is utilized in training symbol design regarding its correlation properties. The time synchronization and channel estimation are achieved by measuring the correlation between the received training sequence and the locally generated training sequence. Repeated training symbols are used to get carrier frequency offset (CFO) estimation. It is shown from the analysis that the accuracy of frequency synchronization is close to the Cramér-Rao lower bound. The training sequences are optimal for channel estimation based on the minimum mean square error (MMSE). 相似文献
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教育高校毕业生树立正确的职业观 总被引:1,自引:0,他引:1
正确的职业观对高校毕业生求职择业、指导职业活动和人生发展具有重要的意义。文中针对加强高校毕业生的思想政治教育,帮助高校毕业生树立服务人民、奉献社会的正确职业观,引导他们用正确的职业观处理求职择业和职业活动中的各种矛盾,选择科学的人生价值目标,重视人生价值的实现,服从社会需要,追求全局利益,发扬艰苦奋斗精神,从而引导高校毕业生提高求职择业质量和水平,并在职业活动中创造出不平凡的业绩进行了分析和论述。 相似文献
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火电厂作为重点抗震设防建筑,理应具有较强的抗震性能,而现行"两阶段"结构设计方法无法提供结构在地震作用下的整体反应。本文采用静力弹塑性分析方法,利用SAP软件,结合我国现行抗震设计规范,分析了火电厂预应力框架在地震作用下的结构反映特性,并通过与拟静力试验结果的对比,提出设计建议。分析表明该结构具有明显的薄弱环节,在设计中应予以重视。 相似文献