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R Faccio M Grano S Colucci AZ Zallone V Quaranta AJ Pelletier 《Canadian Metallurgical Quarterly》1998,249(2):522-525
Integrins mediate cell adhesion and can induce different cellular responses, including changes in intracellular pH, changes and oscillation in intracellular free calcium, and protein phosphorylation on tyrosine. During bone resorption, the integrin alphav beta3 regulates adhesion of osteoclasts to bone extracellular matrix proteins, such us osteopontin (Opn). Adhesion via alphav beta3 is followed by osteoclast polarization onto the bone surface and by the onset of bone resorption. To characterize these events at the molecular level, we investigated the state of activation of alphav beta3 on the human osteoclast-like cell line GCT23 using the monoclonal antibody AP5 which binds to and can induce, under low calcium conditions, activated alphav beta3. By flow cytometry, approximately 50% of alphav beta3 on the surface of the osteoclast-like cell line GCT23 was reactive with AP5 and was therefore in the activated state. Incubation with AP5 in the presence of low calcium concentrations increased activated alphav beta3 to 90-100%. Activation of alphav beta3 increased the efficiency of GCT23 adhesion to Opn by 2-fold. Furthermore, haptotactic migration on Opn was also enhanced about 40% compared to control. We propose that changes in the activation state of alphav beta3 may be a regulation point for osteoclasts during bone resorption. 相似文献
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AHN Gail-Joon 《中国科学:信息科学(英文版)》2011,(8):1608-1617
Proof of retrievability (POR) is a technique for ensuring the integrity of data in outsourced storage services.In this paper,we address the construction of POR protocol on the standard model of interactive proof systems.We propose the first interactive POR scheme to prevent the fraudulence of prover and the leakage of verified data.We also give full proofs of soundness and zero-knowledge properties by constructing a polynomial-time rewindable knowledge extractor under the computational Diffie-Hellman assump... 相似文献
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J. T. TIAN J. M. MOLINA J. NARCISO C. GARCÍA-CORDOVILLA E. LOUIS 《Journal of Materials Science》2005,40(9-10):2537-2540
Oxidizing SiC particles is a common practice in the fabrication of SiC/Al composites through direct mixing of the liquid metal and the ceramic particles. This practice is addressed to reduce the reaction between the two materials. In a previous work it was concluded that oxidizing SiC particles scarcely affected the threshold pressure for infiltration of pure Al into compacts made of those particles. In this work we present a detailed study of this problem for infiltration of pure Al and the eutectic Al-Si alloy. Our results indicate that oxidizing of SiC particles does not affect significantly either the threshold pressure or the infiltration kinetics. This conclusion is equally valid for both pure Al and the eutectic alloy. 相似文献
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ABSTRACT
Over two subsequent seasons, emission of volatile compounds, consumer acceptability and quality parameters were analyzed in “Fuji” apples. In both seasons, apples were harvested at the same maturity stage and stored for 19 or 30 weeks at 1C and 92% relative humidity under an ultralow oxygen (ULO) atmosphere (1% O2 + 1% CO2) or under an ULO atmosphere followed by different periods (2 or 4 weeks) in cold air. The results revealed, for both seasons, that 4 weeks of cold air storage after ULO storage helped to increase the emission of some volatile compounds for both storage period, including characteristic volatile compounds for the variety, and did not have a negative effect on quality parameters. Additionally, the consumer acceptability was superior for those fruits.PRACTICAL APPLICATIONS
It is well known for various apple varieties that long‐term storage under ULO conditions reduces volatile production resulting in poor flavor and aroma compared with fruits stored in air. Consequently, there is often dissatisfaction among consumers with the flavor of fruits which have been subjected to cold storage. The strategy that is proposed in the present work could be recommended to enhance the aromatic quality of fruit provided by the apple industry and, in consequence, increase its acceptability by consumers. 相似文献10.