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排序方式: 共有272条查询结果,搜索用时 15 毫秒
1.
Densitometry determination of dioxins using gold nanoparticlemodified dioxin response element probes
Li-Fan Zhao Li Li Yu-Qiu Gao Bo-Sheng Li Yuan-Yuan Li Chang-Hua Shi Tian Chen Wen-Jie Yang Xiao-Bo Li Shun-Qing Xu 《Gold bulletin》2007,40(4):305-309
This study aims to develop a rapid and sensitive densitometry bioassay of dioxins. It is known that dioxin ligand can bind
heterodimeric aryl hydrocarbon receptor (AhR) and trigger the formation of the complex of dioxin-AhR, AhR nuclear translocator
(Arnt), and dioxin-responsive element (DRE). We have established a rapid and sensitive method using nanoparticle-modified
DRE probes based on dioxin-dependent binding of DRE to AhR complex. Dioxins can trigger the binding of nanoparticle-modified
DRE probe to AhR complex. The nanoparticle-modified DRE probes in the dioxin-AhR-Arnt-DRE complexes immobilized on the microplates
were detected by measuring optical density after silver enhancement. Results showed that as little as 0.01 pM of TCDD could
be detected. Dioxins could be quantified accurately with the linear range from 1 nM to 0.01 pM. The coefficient of variation
was 5≈8% in this bioassay. The optical density of nanoparticle-based silver enhancement was produced in a dose- and time-
dependent manner. The dioxin concentrations in fly ash samples determined by this bioassay were comparable with that by the
standard HRGC/HRMS. Thus this bioassay is a sensitive and convenient cell-free bioassay of dioxin that is suitable for screening
of dioxins in environmental or food matrices. 相似文献
2.
雾天退化图像的复原过程中,针对大气光幕和大气亮度估计不准确导致光晕效应、偏色现象和对比度不足等问题,提出一种结合WLS (weighted least square)滤波与还原控制因子的去雾算法.首先分析WLS滤波器的原理和性能,并用于大气光幕的有效提取;其次利用Sobel算子检测二值化图像边缘,将边缘数目与像素均值同时作为四叉树空间索引的依据,提高大气亮度的估计准确性;最后分析天空出现颜色失衡现象的原因,引入还原控制因子改善视觉效果.实验结果表明,去雾后图像的平均梯度整体提高58.03%,信息熵提高2.88%,运行时间节省50%以上.该方法对含有浓雾、薄雾以及天空等深度复杂的远景图像、近景图像均能得到高对比度、可视度和色彩保真度的恢复效果. 相似文献
3.
Ji-Hong Zhu Wen-Jie Guo Wei-Hong Zhang Tao Liu 《Structural and Multidisciplinary Optimization》2017,56(1):21-45
The purpose of this paper is to present an extended integrated layout and topology optimization method dealing with the multi-frame and multi-component fuselage structure systems design. Considering an aircraft or aerospace fuselage system including main structure, numbers of frames and featured components located on the frames, a simultaneous optimization procedure is proposed here including geometrical design variables of components and frames as well as topological design variables of main structure and frame structures. The multi-point constraints (MPC) scheme is used to simulate the rivets or bolts connecting the components, frames and structures. The finite circle method (FCM) is implemented to avoid the overlaps among different components and frames. Furthermore, to deal with the difficulties of large numbers of non-overlapping constraints, a penalty method is used here to compose the global strain energy and non-overlapping constraints into a single objective function. To guarantee the fuselage system’s balance, the constraint on the system centroid is also introduced into the optimization. Different numerical examples are tested and the optimized solutions have demonstrated the validity and effectiveness of the proposed formulation. 相似文献
4.
5.
Xiao-Yan Chang Xin-Hai Li Long Zhang Hua-Jun Guo Wen-Jie Peng 《Materials Research Bulletin》2005,40(9):1513-1520
Pure and carbon-containing olivine LiMn0.7Fe0.3O4 were synthesized at 600 °C by the method of solid-state reaction. Structure, surface morphology and charge/discharge performance of LiMn0.7Fe0.3O4 were characterized by X-ray diffraction, scanning electron microscopy, and electrochemical measurement, respectively. The prepared materials with and without carbon both show the single olivine structure. The morphologies of primary particles are greatly affected by the addition of carbon. Large particles (500-1000 nm) and densely sintered blocks were observed in pure LiMn0.7Fe0.3PO4, which made the insertion and extraction of lithium ions difficult. Battery made from this sample can not charge and discharge effectively. The carbon-containing LiMn0.7Fe0.3PO4 has a small particle size (100-200 nm) and a regular appearance. This material demonstrates high reversible capacity of about 120 mAh g−1, perfect cycling performance, and excellent rate capability. It is obvious that the addition of carbon plays an important role in restricting the particle size of the material, which helps to prepare LiMn0.7Fe0.3PO4 with excellent electrochemical performance. The electrochemical reaction resistance is much lower in the partly discharged state than in the fully charged or fully discharged state by the measurement of ac impedance for carbon-containing LiMn0.7Fe0.3PO4. It is indicated that the mixed-valence of Fe3+/Fe2+ or Mn3+/Mn2+ is beneficial to the transfer of electron which happens between the interface. 相似文献
6.
Sodium 3-ferrocenyl-5-phenyl-pyrazoline-1-dithiocarbamate (FPPD) reacts with anhydrousrare earth chlorides in absolute ethanol to give 14 new complexes.The results of elemental analysis show thatthe compositions of the complexes are RE(FPPD)_3·nH_2O(RE=La,Pr~Lu and Y;n=3).The ligand andits complexes have been characterized by infrared spectroscopy,UV-visible absorptionspectroscopy,~1H-NMR spectra,thermal analysis and molar conductivity measurement. 相似文献
7.
排序合并连接是数据库系统一种重要的连接实现方式,比哈希连接有更广泛的应用.分布式环境下,数据分片、分布存储,面对昂贵的网络代价,进行高效排序合并连接的挑战巨大.传统策略首先针对连接数据进行排序,然后基于排好序的数据执行合并连接.这两部分操作均基于原始数据进行操作,通常情况下,原始连接数据存在无用数据块,这些数据块无需连接,但会增加额外开销,包括网络开销.随着数据量的增多,出现无用数据块的概率增大,额外开销随之增多.传统策略没有预先处理这些无用数据块.针对这个问题,提出一种分布式环境下基于剪枝的并行排序合并连接策略(parallel sort-merge join based on prune,简称Pr_PSMJ).其特点是,连接发生之前高效完成对连接对象无用数据块的剪枝处理,提高整体连接效率.基本思想是,根据连接对象对应的连接分区数据统计信息,构造一种双边邻接表(bilateral adjacency list,简称BAL),用来对连接数据中无用数据块进行剪枝,并保证最终连接结果的正确性;剪枝完成后,利用BAL计算出各个最佳本地连接执行点,并指导分区数据的迁移,使数据移动量最小;在连接阶段,由于BAL保证本地连接执行节点的独立性,因此能够轻松并行执行整个连接过程,并在每个连接点本地利用多核环境完成局部并行排序合并连接;最后,将局部结果合并成最终结果.由于Pr_PSMJ中的高效剪枝策略是在连接执行之前完成的,因此几乎适合任何合并连接操作,并且对于其他连接策略也有借鉴作用.给出了基于Pr_PSMJ的算法的正确性、效率性以及适应性分析,并且给出实验验证,证明了在分布式大数据量排序合并连接情况下,Pr_PSMJ相对于其他策略能够有效减少网络开销,并提高连接效率. 相似文献
8.
本文分析了聚类的思想,将免疫原理引入到遗传算法并应用于聚类分析过程中,提出了改进的免疫遗传算法(MIGA).该算法借鉴了免疫算法中有关浓度的定义,并使用了GA算法中交叉和变异的思想.通过实验显示该方法优于基本的遗传算法. 相似文献
9.
10.
量子可逆逻辑电路综合的快速算法研究 总被引:4,自引:0,他引:4
可逆逻辑有许多应用,尤其在量子计算领域,量子可逆逻辑电路是构建量子计算机的基本单元,量子可逆逻辑电路综合就是根据电路功能,以较小的量子代价自动构造量子可逆逻辑电路.文中结合可逆逻辑电路综合的多种算法,提出了一种新颖高效的算法,自动构造正极性Reed-Muller展开式(RM),在生成量子可逆逻辑电路的解空间树上,采用总体层次遍历,局部深度搜索,借鉴模板优化技术,构造限界函数快速剪去无解或非最优解的分枝,优先探测RM中的因子,以极高的效率生成最优电路.以国际公认的3变量可逆函数测试标准,该算法不仅能够生成全部最优电路,而且运行速度远远超过同类算法. 相似文献