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A new sub-class of binding protein-dependent transporter with specificity for a broad range of polar amino acids has been identified by sequence comparison, in Rhizobium leguminosarum, Rhodobacter capsulatus, Escherichia coli and Pseudomonas fluorescens. Southern blotting and PCR analysis has shown that transporters from this new sub-class are widely distributed in Gram-negative bacteria, including, in addition to the above, Citrobacter freundii, Erwinia carotovorum and Rhizobium meliloti. ABC transporters of polar amino acids can be divided into two groups: those with narrow solute specificity and the newly identified sub-class with broad solute specificity. The binding and inner membrane proteins from transporters with a broad solute specificity are larger by approximately 30% than those with a narrow solute specificity. Multiple alignment of the inner membrane proteins from all sequenced polar amino acid transporters indicates there is an N-terminal conserved region that may be involved in solute specificity. A conserved arginine or lysine at residue 30 of this region is changed to glutamate in arginine transporters. Residue 53 also has a strong correlation with the charge on the transported solute, with basic amino acid transporters replacing an aliphatic amino acid at this position with a negatively charged amino acid. The general amino acid permease from R. leguminosarum, which will transport aliphatic as well as basic and acidic amino acids, juxtaposes two prolines at residues 52 and 53 of the N-terminal conserved region. 相似文献
4.
Sigal Gottlieb Julia S. Mullen Steven J. Ruuth 《Journal of scientific computing》2006,27(1-3):271-287
The weighted essentially non-oscillatory (WENO) method is an excellent spatial discretization for hyperbolic partial differential equations with discontinuous solutions. However, the time-step restriction associated with explicit methods may pose severe limitations on their use in applications requiring large scale computations. An efficient implicit WENO method is necessary. In this paper, we propose a prototype flux-implicit WENO (iWENO) method. Numerical tests on classical scalar equations show that this is a viable and stable method, which requires appropriate time-stepping methods. Future study will include the examination of such methods as well as extension of iWENO to systems and higher dimensional problems.Sigal Gottlieb - The work of this author supported by NSF grant DMS-0106743.Steven J. Ruuth - The work of this author was partially supported by a grant from NSERC Canada. 相似文献
5.
Knapp Samuel; Gottlieb Michael; Berman Jason; Handelsman Mitchell M. 《Canadian Metallurgical Quarterly》2007,38(1):54
At times the laws under which psychologists function may appear to contradict generally recognized ethical values and/or good clinical care. When these circumstances arise, psychologists must determine if a conflict really exists and, if so, seek solutions that reconcile respect for the law with their ethical values. At times, psychologists may decide to follow the law despite their ethical concerns. At other times, they may determine that a conscientious objection is warranted. The authors recommend options to consider when these situations arise and offer a decision-making process. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
6.
Investigated the weight histories of obese diabetic outpatients. Retrospective self-reports of 152 patients indicated that about one-third could be classified as cured of their obesity, and an additional 26%, although still obese, weighed 10% less than they had at their maximum. Medical records of 219 Ss revealed that less than 10% could be considered cured and only another 12% partially cured. Although some support was found for the hypothesized relationship between weight-loss success and time in treatment, it is possible that this reflects a tendency for those initially successful to remain in treatment longer. Finally, maximum weights of at least 30% over ideal were associated with more success, primarily in achieving a 10% weight loss rather than in being cured of obesity. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
7.
Previous studies have revealed that if mallard ducklings are given social experience with broodmates after their initial exposure to a stuffed mallard (Anas platyrhynchos) hen, they later show a visual preference for the familiar mallard over an unfamiliar redhead (Aythya americana) model. Birds kept in isolation do not make this difficult discrimination. We examined when the social experience with siblings must occur in order to contribute to maternal imprinting. We allowed 24-hr-old domestic mallard ducklings to follow a stuffed mallard hen for 30 min (training). This experience resulted in a preference for the familiar mallard over the unfamiliar redhead hen in a simultaneous choice test 48 hrs after hatching only if the birds were allowed social experience with broodmates after the training trial (i.e., from 24 to 48 hrs). Thus the visual features of the mallard hen that are necessary in order to distinguish it from the redhead hen become consolidated only after more than 1 hr into the posttraining social rearing; we term this process retroactive excitation. These results provide evidence of a nonobvious experiential contribution that conspecifics make to the development of species-typical social behavior. They also suggest that some learning and memorial processes may operate rather differently in adults and immature animals. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
8.
LJ Bendall V Makrynikola A Hutchinson AC Bianchi KF Bradstock DJ Gottlieb 《Canadian Metallurgical Quarterly》1998,12(9):1375-1382
Acute myeloid leukaemia (AML) cells express the SCF receptor c-kit (CD117) on their cell surface and demonstrate enhanced adhesion to fibronectin (FN) following exposure to stem cell factor (SCF). Increased adhesion occurs within 5 min, is dose dependent, and persists beyond 2 h. Baseline and enhanced adhesion occur through the surface FN receptor very late antigen-5 (VLA-5, CD49e/CD29) which is expressed by AML cells. Unstimulated AML cells exposed to FN undergo less apoptosis than controls (inhibition 22.5 +/- 7.0%, P = 0.02, n = 8). Exposure to SCF alone without FN also inhibits AML cell apoptosis (by 19.0 +/- 7.7% compared to controls, P = 0.06, n = 8). Simultaneous exposure to SCF and FN increases the inhibition of AML cell apoptosis to 37.8 +/- 7.9% (P = 0.005 compared to control, P = 0.04 compared to FN alone, P = 0.06 compared to SCF alone) demonstrating that SCF not only enhances the propensity of AML cells to adhere to FN, but also results in an additive survival benefit following FN contact. Some but not all the reduction in apoptosis is mediated through VLA-5. The combination of SCF and FN also affects proliferation, resulting in a synergistic enhancement of AML cell proliferation in half the cases studied. When normal CD34+ human haemopoietic progenitors were studied, FN had little effect on their apoptosis and failed to enhance the anti-apoptotic effect of SCF. It did, however, synergise with SCF in promoting CD34+ cell proliferation. Exposure of AML cells to SCF and FN, both of which can be found in high concentration in the bone marrow stroma, inhibits apoptosis. Cytokines and extracellular matrix proteins augment each others' effects since SCF enhances adhesion to fibronectin, which in turn augments the survival signal delivered by the cytokine alone. Cytokine and adhesion receptors can combine to affect cell characteristics including proliferation and survival. 相似文献
9.
MB Gottlieb JE Blaugrund JK Niparko 《Canadian Metallurgical Quarterly》1998,124(11):1274, 1276-1274, 1277
10.
Colin B. Macdonald Sigal Gottlieb Steven J. Ruuth 《Journal of scientific computing》2008,36(1):89-112
Diagonally split Runge–Kutta (DSRK) time discretization methods are a class of implicit time-stepping schemes which offer
both high-order convergence and a form of nonlinear stability known as unconditional contractivity. This combination is not
possible within the classes of Runge–Kutta or linear multistep methods and therefore appears promising for the strong stability
preserving (SSP) time-stepping community which is generally concerned with computing oscillation-free numerical solutions
of PDEs. Using a variety of numerical test problems, we show that although second- and third-order unconditionally contractive
DSRK methods do preserve the strong stability property for all time step-sizes, they suffer from order reduction at large
step-sizes. Indeed, for time-steps larger than those typically chosen for explicit methods, these DSRK methods behave like
first-order implicit methods. This is unfortunate, because it is precisely to allow a large time-step that we choose to use
implicit methods. These results suggest that unconditionally contractive DSRK methods are limited in usefulness as they are
unable to compete with either the first-order backward Euler method for large step-sizes or with Crank–Nicolson or high-order
explicit SSP Runge–Kutta methods for smaller step-sizes.
We also present stage order conditions for DSRK methods and show that the observed order reduction is associated with the
necessarily low stage order of the unconditionally contractive DSRK methods.
The work of C.B. Macdonald was partially supported by an NSERC Canada PGS-D scholarship, a grant from NSERC Canada, and a
scholarship from the Pacific Institute for the Mathematical Sciences (PIMS).
The work of S. Gottlieb was supported by AFOSR grant number FA9550-06-1-0255.
The work of S.J. Ruuth was partially supported by a grant from NSERC Canada. 相似文献