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1.
Two commercial Pt modified aluminide coatings (RT22 and MDC150L) on the same single crystal Ni-based superalloy (CMSX-4) were studied by: scanning electron microscopy; transmission electron microscopy; energy dispersive X-ray spectrometry; and gravimetry. The RT22 coating is an inward grown coating (high activity), while MDC150L is produced by outward growth (low activity). Samples were oxidised in still laboratory air at 1050 °C for various times up to 2000 h. It was found that the outward grown coating produced a slower growing oxide that was more spallation resistant. Several possible reasons for this were identified including: coating purity; coating surface topography; and ductile to brittle transition temperature. The microstructural differences between the two coatings in the as-coated condition were investigated and the development of their microstructure during heat treatment was described. A model for coating growth during heat treatment was proposed. 相似文献
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简述了上海汽轮机有限公司(STC)与西门子公司首次合作制造的目前国内最大容量的外高桥900MW超临界机组的一些基本情况,介绍了在合作制造中为保证生产进度和质量而采取的工艺改进、技术攻关等措施,以及通过合作制造提升了我公司的制造能力和管理水平。 相似文献
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The influence of Glu44 and Glu56 of cytochrome b5 on the protein structure and interaction with cytochrome c 总被引:1,自引:0,他引:1
Sun Yu-Long; Xie Yi; Wang Yun-Hua; Xiao Gu-Tian; Huang Zhong-Xian 《Protein engineering, design & selection : PEDS》1996,9(7):555-558
The gene encoding trypsin-solubilized bovine liver microsomalcytochrome b5 (82 residues in length) has been mutated, in whichthe codons of Glu44 and Glu56 were changed to those of Ala.The mutated genes were expressed in Escherichia coli successfullyand three mutant proteins (E44A, E56A and E44/56A) were obtained.The UV-visible, CD and 1H NMR spectra of proteins have beenstudied. The results show that the mutagenesis at surface residuesdoes not alter the secondary and tertiary structures of cytochromeb5 significantly. The interactions between recombinant cytochromeb5 and its mutants with cytochrome c were studied by using opticaldifference spectra. The results demonstrated that both Glu44and Glu56 of cytochrome b5 participate in the formation of acomplex between cytochrome b5 and cytochrome c. 相似文献
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Kojima Shuichi; Takagi Nobuyuki; Minagawa Tetsuya; Fushimi Noriko; Miura Kin-ichiro 《Protein engineering, design & selection : PEDS》1999,12(10):857-862
We have previously shown that replacing the P1-site residue(Ala) of chicken ovomucoid domain 3 (OMCHI3) with a Met or Lysresults in the acquisition of inhibitory activity toward chymotrypsinor trypsin, respectively. However, the inhibitory activitiesthus induced are not strong. In the present study, we introducedadditional amino acid replacements around the reactive siteto try to make the P1-site mutants more effective inhibitorsof chymotrypsin or trypsin. The amino acid replacement AspTyrat the P2' site of OMCHI3(P1Met) resulted in conversion to a35000-fold more effective inhibitor of chymotrypsin with aninhibitor constant (Ki) of 1.17x1011 M. The Ki valueof OMCHI3(P1Met, P2'Ala) indicated that the effect on the interactionwith chymotrypsin of removing a negative charge from the P2'site was greater than that of introducing an aromatic ring.Similarly, enhanced inhibition of trypsin was observed whenthe AspTyr replacement was introduced into the P2' site of OMCHI3(P1Lys).Two additional replacements, AspAla at the P4 site and ArgAlaat the P3' site, made the mutant a more effective inhibitorof trypsin with a Ki value of 1.44x109 M. By contrast,ArgAla replacement at the P3' site of OMCHI3(P1Met, P2'Tyr)resulted in a greatly reduced inhibition of chymotrypsin, andAspAla replacement at the P4 site produced only a small changewhen compared with a natural variant of OMCHI3. These resultsclearly indicate that not only the P1-site residue but alsothe characteristics, particularly the electrostatic properties,of the amino acid residues around the reactive site of the proteaseinhibitor determine the strength of its interactions with proteases.Furthermore, amino acids with different characteristics arerequired around the reactive site for strong inhibition of chymotrypsinand trypsin. 相似文献
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介绍了国电铜陵600 MW超临界燃煤机组安装脱硝装置项目的前期研究、主要设计特点、系统设计、调试运行,以及投产运行后的经济情况,得出了一系列火电项目加装脱硝设施建设与运行方面的参考意见. 相似文献
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广东某新建1 000 MW超超临界发电机组是上海锅炉厂有限公司引进美国AISTOM技术设计的首台Ⅱ型锅炉.对于高加至省煤器入口高压给水管道的规格设计,由于设计院和锅炉厂二者设计压力等级不同,导致这部分高压给水管道与锅炉主给水门规格存在较大差异,给安装接口工作带来极大的障碍,被迫采用大小头来过渡,造成不必要的浪费和安全隐患.对该管段的设计进行了初步探讨. 相似文献
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为了准确地获得空载和额定负载时的励磁电流,在充分考虑直交轴磁场饱和效应的基础上,利用时变电磁场有限元法计算1000MW水轮发电机在空载和额定负载时的励磁电流.分析了励磁绕组尺寸、阻尼条节距比以及磁性槽楔等相关因素对励磁电流的影响,在获得空载和额定负载励磁电流的基础上,提出了励磁绕组尺寸、阻尼条节距比的设计准则,并从电磁设计的角度论证了磁性槽楔应用于1000MW水轮发电机上的可行性.实验结果表明,有限元法可以用于1000MW水轮发电机的励磁电流计算,为工程设计提供了理论依据. 相似文献