GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice

The lysosomal storage disease Niemann–Pick type C (NPC) is caused by impaired cholesterol efflux from lysosomes, which is accompanied by secondary lysosomal accumulation of sphingomyelin and glucosylceramide (GlcCer). Similar to Gaucher disease (GD), patients deficient in glucocerebrosidase (GCase) degrading GlcCer, NPC patients show an elevated glucosylsphingosine and glucosylated cholesterol. In livers of mice lacking the lysosomal cholesterol efflux transporter NPC1, we investigated the expression of established biomarkers of lipid-laden macrophages of GD patients, their GCase status, and content on the cytosol facing glucosylceramidase GBA2 and lysosomal integral membrane protein type B (LIMP2), a transporter of newly formed GCase to lysosomes. Livers of 80-week-old Npc1^−/− mice showed a partially reduced GCase protein and enzymatic activity. In contrast, GBA2 levels tended to be reciprocally increased with the GCase deficiency. In Npc1^−/− liver, increased expression of lysosomal enzymes (cathepsin D, acid ceramidase) was observed as well as increased markers of lipid-stressed macrophages (GPNMB and galectin-3). Immunohistochemistry showed that the latter markers are expressed by lipid laden Kupffer cells. Earlier reported increase of LIMP2 in Npc1^−/− liver was confirmed. Unexpectedly, immunohistochemistry showed that LIMP2 is particularly overexpressed in the hepatocytes of the Npc1^−/− liver. LIMP2 in these hepatocytes seems not to only localize to (endo)lysosomes. The recent recognition that LIMP2 harbors a cholesterol channel prompts the speculation that LIMP2 in Npc1^−/− hepatocytes might mediate export of cholesterol into the bile and thus protects the hepatocytes.     

基于图灵模型的P=？ NP问题分析

P=？NP问题是计算复杂性中的核心问题。2000年,美国克雷实验室将其收录为“千禧年大奖”七个问题之首。本文基于图灵模型,对P=？NP问题的研究现状、P=NP／P≠NP 证明方法、NPC问题求解方法及研究进展进行阐述。     

基于I-DPWM的三电平中点钳位光伏逆变器漏电流抑制方法

对于三电平中点钳位光伏逆变器,基于断续脉宽调制(discontinuous pulse width modulation, DPWM)并通过选择特定矢量进行钳位控制的方法,存在矢量分解计算时间长、注入共模电压分量和漏电流过大等不足。对此,提出了一种基于I-DPWM的三电平中点钳位光伏逆变器漏电流抑制方法。通过计算各类DPWM等效调制波来解析注入的低频共模电压分量。针对低频共模电压分量最小的DPWM1,在低频共模电压不连续点注入新的零序分量,通过减小低频共模电压来抑制漏电流幅值。同时,采用载波实现DPWM,免去了复杂的矢量运算。最后,在Matlab/Simulink平台建立了20 kW三电平中点钳位光伏逆变器的仿真模型,同时结合所设计的125 W实验样机验证了所提方法的有效性。     

也谈高强度公钥密码体制的研究

文章对刊载于《计算机工程与应用》2003年第3期、题名为“基于双密钥的高强度公钥密码体制的研究”的文章进行了分析,指出该文提出的将RSA和Elgamal串起来用的公钥密码体制不能增加安全强度。同时,文章阐述了目前公钥密码体制的安全现状和今后的研究方向。     

Power conversion system for high altitude wind power generation with medium voltage AC transmission

High Altitude Wind Power (HAWP) generating system provides clean energy at low cost and high capacity factor due to reduced size of the turbine and high speed streamlined wind at high altitude. An air-borne wind turbine (AWT) at high altitude extracts kinetic energy from wind using buoyancy provided by the blimp/aerostat. The generated electrical power is then transmitted to the ground based station (without any power conditioning) using the transmission lines (tether). The power conversion system (PCS) for harnessing HAWP is proposed in this paper. The proposed PCS consists of a three-level neutral point clamped (NPC) rectifier, a three-level NPC DC–DC converter followed by a two-level inverter. Modelling, design and control of the PCS are presented and discussed. The PCS provides generation side maximum power-point tracking (MPPT) using sensorless optimal torque control technique. The DC–DC converter provides electrical isolation as well as voltage step-down functions. A modified proportional resonant (PR) control which can selectively eliminate lower order current harmonics of the grid-connected inverter is also presented. The proposed control scheme of the PCS is evaluated through simulation studies using software programs like PSIM and MATLAB. A scaled-down 1 kW laboratory prototype of the complete PCS is designed, built and tested. The experimental test results obtained validate the proposed control scheme for efficient power generation from high altitude wind and interface to the grid/load.     

N-enriched porous carbon doped with co,ni, and Mo as efficient electrocatalyst for hydrogen evolution reaction

Splitting water for hydrogen production is still a promising technique to meet the energy requirements of society and overcome many environmental problems. However, the development of carbon-based transition electrocatalysts with superior activity for hydrogen evolution reaction (HER) is still challenging. In this study, a CoNiMo/NPC electrocatalyst was successfully fabricated using ZIF-67 as a precursor via facile absorption, pyrolysis and annealing processes. The fabricated CoNiMo/NPC was used as an electrocatalyst for hydrogen production. The results revealed that the doping of Ni and Mo increase the number of active sites and enhance the conductivity of electrocatalysts. CoNiMo/NPC exhibits excellent HER activity in alkaline solutions and only requires an overpotential of 182 mV to reach a current density of 10 mA/cm². Furthermore, long-term measurements demonstrated that CoNiMo/NPC has superior durability in alkaline solutions. The excellent HER performance of CoNiMo/NPC can be attributed to the doping of Co, Ni, and Mo on porous carbon. In addition, the high specific surface area and high graphitisation degree of the electrocatalyst are beneficial for rapid charge transport and collection.     

一种改进的求解TSP问题的近似算法

旅行商问题(TSP)是典型的具有NPC复杂性的组合优化问题。在现有求解TSP问题的2-近似算法closest-point算法基础上,通过对插入点的插入位置进行改进,提出了一种有效的近似算法最近点前后插入法(CPBOA),并采用TSPLIB中的一些典型实例对该算法进行了测试,同时与典型的常数近似比算法MST-PRIM算法和closest-point算法进行了比较。实验结果表明,该算法在求解质量上与closest-point和MST-PRIM算法相比都有很大的改进,而且速度也很快。     

人大对法院监督中存在的问题及其对策

从探讨人大对法院监督的法理基础入手，分析了人大对法院监督中存在的问题，并有针对性地提出了对策。     

Development of hybrid energy system with cycle charging strategy using particle swarm optimization for a remote area in India

In recent years, renewable energy can be seen as one of the important prospect of today's research, as it is likely to enlighten the lives of millions of people by fulfilling demand of electricity in their daily life. The present work focuses on the development of optimal hybrid energy system sizing model based on comparative analysis of particle swarm optimization, genetic algorithm and Homer software for energy index ratio of 1. The model also incorporates renewable fraction, emissions of carbon di oxide from diesel generator, net present cost and cost of energy. The system is developed to supply the demand of 7 un-electrified villages of Dhauladevi block of Almora district in Uttarakhand, India with the help of the available resources of solar, hydro, biomass and biogas energy along with the addition of diesel generator, for meeting out the energy deficit. From the optimization results, minimum cost of energy and maximum renewable fraction are obtained as 5.77 Rs/kWh and 92.6% respectively.     

A circulating current control scheme for multiple three‐level NPC inverter systems

In recent years, motor drive systems for steel rolling mills have been steadily increasing in capacity. In particular, the development of three‐level NPC (Neutral Point Clamped) inverters is now focusing on the replacement of cycloconverters. In order to satisfy such a requirement, in this paper a multiple three‐level NPC inverter system is proposed. The configuration consists of two three‐level NPC inverters with the same configuration connected in parallel by current limiting reactors. In this case, the circulating current flowing between the two three‐level NPC inverters must be controlled to prevent a decrease in the maximum output capacity. In particular, a new method for control of the circulating current is a major topic of discussion. The method is based on space voltage vector‐based PWM (Pulse Width Modulation) control for the three‐level NPC inverters and the circulating current is controlled by adjusting the output durations of the respective space voltage vectors. In this method the circulating current controller can be decoupled from the motor current controller. Therefore, it is possible to design the different controllers independently. In addition, the experimental results obtained using an induction motor show that the circulating current can be reduced to within 2% of the rated motor current when the motor current response exceeds 1000 rad/s. © 2001 Scripta Technica, Electr Eng Jpn, 136(2): 38–48, 2001