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日本基恩士公司生产的CV-2100高速影像处理系统能够实现尺寸检测,具有高速度,高精度的优点.管状类产品(如药柱、轴承等)的内外径尺寸对产品质量具有重要影响,是产品生产中非常重要的几何参数.本论文以圆环的内外径尺寸为测量对象,利用计算机控制CV-2100影像处理系统,实现了对圆环内外径尺寸的测量.并利用计算机VC 6.0编程,验证了该影像系统的测量准确度.实验结果表明,CV-2100影像处理系统检测精度比人工检测精度高,可用于生产中的实时测量. 相似文献
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侧钻是降低油气开采成本的有效途径之一。西北油田受井眼尺寸、避水要求、不稳定泥岩、复杂压力体系等原因的影响,侧钻难度极大。为解决这一难题,在TH10233CH井采用膨胀管技术进行了实际应用。采用φ139.7mm膨胀管封固上部不稳定地层,膨胀管膨胀前内径124.2mm,膨胀后内径可达134.5mm,采用φ130mm钻头钻进,顺利完钻。膨胀管钻井技术在TH10233CH井的成功应用对西北油田侧钻井钻进具有重大指导意义。 相似文献
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目的:研究辅助性T细胞(TH1、TH2)在癌性腹水患者中的作用及临床意义。方法:采用ELISA(酶联免疫吸附法)检测患者外周血和腹水中γ-干扰素(γ-INF)、白细胞介素(IL-4)水平,从而判断TH1、TH2细胞的活性。结果:癌性腹水患者外周血TH1细胞的活性较正常人明显降低,TH2细胞活性明显增强。治疗无效者外周血TH1细胞的活性进一步下降,TH2细胞活性进一步增强,而治疗有效者外周血TH1细胞功能逐渐增强,TH2细胞功能逐渐下降,其水平均接近于正常对照组。结论:外周血辅助性T细胞(TH1、TH2)的活性对癌性腹水患者的疗效及预后判断具有一定的价值。 相似文献
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Marlene Davis Ekpo Jingxian Xie Yuying Hu Xiangjian Liu Fenglin Liu Jia Xiang Rui Zhao Bo Wang Songwen Tan 《International journal of molecular sciences》2022,23(5)
Antifreeze proteins (AFPs) or thermal hysteresis (TH) proteins are biomolecular gifts of nature to sustain life in extremely cold environments. This family of peptides, glycopeptides and proteins produced by diverse organisms including bacteria, yeast, insects and fish act by non-colligatively depressing the freezing temperature of the water below its melting point in a process termed thermal hysteresis which is then responsible for ice crystal equilibrium and inhibition of ice recrystallisation; the major cause of cell dehydration, membrane rupture and subsequent cryodamage. Scientists on the other hand have been exploring various substances as cryoprotectants. Some of the cryoprotectants in use include trehalose, dimethyl sulfoxide (DMSO), ethylene glycol (EG), sucrose, propylene glycol (PG) and glycerol but their extensive application is limited mostly by toxicity, thus fueling the quest for better cryoprotectants. Hence, extracting or synthesizing antifreeze protein and testing their cryoprotective activity has become a popular topic among researchers. Research concerning AFPs encompasses lots of effort ranging from understanding their sources and mechanism of action, extraction and purification/synthesis to structural elucidation with the aim of achieving better outcomes in cryopreservation. This review explores the potential clinical application of AFPs in the cryopreservation of different cells, tissues and organs. Here, we discuss novel approaches, identify research gaps and propose future research directions in the application of AFPs based on recent studies with the aim of achieving successful clinical and commercial use of AFPs in the future. 相似文献
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CHANG Min PENG Dan XU Ke-xin 《光电子快报》2007,3(3):211-214
A double beam near-infrared spectrometer is developed to compensate the water absorption and instrumental drift in intensity. The spectrometer may be used for both single and double beam measurements,and the two operation modes are compared. The results show that the double beam technique eliminates instrumental drift in the single beam measurement and therefore the stability of the system increases by more than 20%. The compensation of the double beam system on water absorption is verified by the measurement of fat content in milk. The results show that the spectrum data based on double beam mode get better calibration model and lower prediction error than traditional single beam mode. 相似文献
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Mohamed F. Elshal Alia M. Aldahlawi Omar I. Saadah J. Philip McCoy 《International journal of molecular sciences》2015,16(12):28998-29010
Loss of tolerance of the adaptive immune system towards indigenous flora contributes to the development of inflammatory bowel diseases (IBD). Defects in dendritic cell (DC)-mediated innate and adoptive immune responses are conceivable. The aim of this study was to investigate the expression of the inhibitory molecules CD200R1 and their ligand CD200 on DCs, to clarify the role of the DCs in the pathogenesis of IBD. Thirty-seven pediatric IBD patients (23 with Crohn’s disease (CD) and 14 with ulcerative colitis (UC)) with mean age 13.25 ± 2.9 years were included. Fourteen age-matched healthy pediatric volunteers (five males and nine females) served as a control group (HC). The percentage of CD11c+ myeloid dendritic cells (mDCs) and CD123+ plasmacytoid DCs (pDCs) expressing CD200R1 and CD200 were evaluated in peripheral blood using flow cytometry and were correlated with routine biochemical, serological markers, serum levels of cytokines and with the percentages of circulating regulatory T cells (Treg) and CD4+ producing IL-17 (Th17). IBD patients showed a significant decrease in the percentage of pDCs and mDCs expressing CD200R1 compared to that of HC. Patients with UC showed increased expressions of the CD200 molecule on pDCs as compared to HC. DCs expressing CD200R1 were found to be correlated positively with Treg and negatively with TH17 and erythrocyte sedimentation rate (ESR). Our findings suggest that IBD is associated with dysregulation in the CD200R1/CD200 axis and that the decrease in DCs expressing CD200R1 may contribute to the imbalance of Th17 and Treg cells and in the pathogenesis of IBD. 相似文献
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Exposure and effects assessment of persistent organohalogen contaminants in arctic wildlife and fish 总被引:1,自引:0,他引:1
Robert J. Letcher Jan Ove Bustnes Christian Sonne Mathilakath M. Vijayan 《The Science of the total environment》2010,408(15):2995-10202
Persistent organic pollutants (POPs) encompass an array of anthropogenic organic and elemental substances and their degradation and metabolic byproducts that have been found in the tissues of exposed animals, especially POPs categorized as organohalogen contaminants (OHCs). OHCs have been of concern in the circumpolar arctic for decades. For example, as a consequence of bioaccumulation and in some cases biomagnification of legacy (e.g., chlorinated PCBs, DDTs and CHLs) and emerging (e.g., brominated flame retardants (BFRs) and in particular polybrominated diphenyl ethers (PBDEs) and perfluorinated compounds (PFCs) including perfluorooctane sulfonate (PFOS) and perfluorooctanic acid (PFOA) found in Arctic biota and humans. Of high concern are the potential biological effects of these contaminants in exposed Arctic wildlife and fish. As concluded in the last review in 2004 for the Arctic Monitoring and Assessment Program (AMAP) on the effects of POPs in Arctic wildlife, prior to 1997, biological effects data were minimal and insufficient at any level of biological organization. The present review summarizes recent studies on biological effects in relation to OHC exposure, and attempts to assess known tissue/body compartment concentration data in the context of possible threshold levels of effects to evaluate the risks. This review concentrates mainly on post-2002, new OHC effects data in Arctic wildlife and fish, and is largely based on recently available effects data for populations of several top trophic level species, including seabirds (e.g., glaucous gull (Larus hyperboreus)), polar bears (Ursus maritimus), polar (Arctic) fox (Vulpes lagopus), and Arctic charr (Salvelinus alpinus), as well as semi-captive studies on sled dogs (Canis familiaris). Regardless, there remains a dearth of data on true contaminant exposure, cause-effect relationships with respect to these contaminant exposures in Arctic wildlife and fish. Indications of exposure effects are largely based on correlations between biomarker endpoints (e.g., biochemical processes related to the immune and endocrine system, pathological changes in tissues and reproduction and development) and tissue residue levels of OHCs (e.g., PCBs, DDTs, CHLs, PBDEs and in a few cases perfluorinated carboxylic acids (PFCAs) and perfluorinated sulfonates (PFSAs)). Some exceptions include semi-field studies on comparative contaminant effects of control and exposed cohorts of captive Greenland sled dogs, and performance studies mimicking environmentally relevant PCB concentrations in Arctic charr. Recent tissue concentrations in several arctic marine mammal species and populations exceed a general threshold level of concern of 1 part-per-million (ppm), but a clear evidence of a POP/OHC-related stress in these populations remains to be confirmed. There remains minimal evidence that OHCs are having widespread effects on the health of Arctic organisms, with the possible exception of East Greenland and Svalbard polar bears and Svalbard glaucous gulls. However, the true (if any real) effects of POPs in Arctic wildlife have to be put into the context of other environmental, ecological and physiological stressors (both anthropogenic and natural) that render an overall complex picture. For instance, seasonal changes in food intake and corresponding cycles of fattening and emaciation seen in Arctic animals can modify contaminant tissue distribution and toxicokinetics (contaminant deposition, metabolism and depuration). Also, other factors, including impact of climate change (seasonal ice and temperature changes, and connection to food web changes, nutrition, etc. in exposed biota), disease, species invasion and the connection to disease resistance will impact toxicant exposure. Overall, further research and better understanding of POP/OHC impact on animal performance in Arctic biota are recommended. Regardless, it could be argued that Arctic wildlife and fish at the highest potential risk of POP/OHC exposure and mediated effects are East Greenland, Svalbard and (West and South) Hudson Bay polar bears, Alaskan and Northern Norway killer whales, several species of gulls and other seabirds from the Svalbard area, Northern Norway, East Greenland, the Kara Sea and/or the Canadian central high Arctic, East Greenland ringed seal and a few populations of Arctic charr and Greenland shark. 相似文献