Spinal Excitatory Dynorphinergic Interneurons Contribute to Burn Injury-Induced Nociception Mediated by Phosphorylated Histone 3 at Serine 10 in Rodents

The phosphorylation of serine 10 in histone 3 (p-S10H3) has recently been demonstrated to participate in spinal nociceptive processing. However, superficial dorsal horn (SDH) neurons involved in p-S10H3-mediated nociception have not been fully characterized. In the present work, we combined immunohistochemistry, in situ hybridization with the retrograde labeling of projection neurons to reveal the subset of dorsal horn neurons presenting an elevated level of p-S10H3 in response to noxious heat (60 °C), causing burn injury. Projection neurons only represented a small percentage (5%) of p-S10H3-positive cells, while the greater part of them belonged to excitatory SDH interneurons. The combined immunolabeling of p-S10H3 with markers of already established interneuronal classes of the SDH revealed that the largest subset of neurons with burn injury-induced p-S10H3 expression was dynorphin immunopositive in mice. Furthermore, the majority of p-S10H3-expressing dynorphinergic neurons proved to be excitatory, as they lacked Pax-2 and showed Lmx1b-immunopositivity. Thus, we showed that neurochemically heterogeneous SDH neurons exhibit the upregulation of p-S10H3 shortly after noxious heat-induced burn injury and consequential tissue damage, and that a dedicated subset of excitatory dynorphinergic neurons is likely a key player in the development of central sensitization via the p-S10H3 mediated pathway.     

Investigation of the use of ram horn hydrolysate for fungal protein production

Production of the fungus Aspergillus niger NRRL 330 was studied in submerged fermentation with ram horn hydrolysate (RHH) as substrate. The characteristics of RHH have been reported previously. The RHH was enriched by addition of glucose and KH₂PO₄. The effects of kinetic parameters on the biomass yield of the fungus were investigated. The optimal conditions for growth of A niger on RHH were initial pH 6.5, temperature 30 °C, fermentation time 96 h and agitation speed 150 rpm. Under these optimal conditions the initial carbohydrate content of RHH was reduced from 1.52 to 0.2% and the biomass yield was 8.9 g l^?1. The biomass contained about 48.1% protein, 5.2% fat and 9.4% ash (on a dry weight basis). The amino acid content of the biomass was compared with Food and Agricultural Organisation (FAO) and animal feed standards. The protein produced contained all the essential amino acids for animal feed, but the amounts of these amino acids were somewhat lower than those of FAO and soybean reference protein. However, the amino acid composition of the biomass was better than that of animal feed. The results with RHH were also compared with previously reported data on fungal mycelium grown on waste liquor substrate. In conclusion, it was found that RHH could be used as a substrate in the production of fungal protein for use as animal feed. Copyright © 2003 Society of Chemical Industry     

新型圆锥加脊TEM喇叭天线的分析

ＴＥＭ喇叭是一种超宽带天线，在瞬态场和超宽带雷达的研究中具有良好的理论和应用价值，本文首先应用格林函数法分析了壁无限薄的圆锥加脊ＴＥＭ喇叭天线，计算了喇叭上的电荷分布及截面阻抗，并分析这种ＴＥＭ喇叭天线在不同辐射方向的波形，结果表明这 超宽带和波形保真性。     

碳纤维喇叭天线制造工艺研究

喇叭天线是雷达系统中重要的功能件,碳纤维材料由于具有金属材料不具备的某些优异性能,在雷达系统中的应用越来越广泛。文中介绍了一种碳纤维喇叭天线的制造工艺过程,对喇叭天线原型结构的工艺改进设计满足碳纤维材料热压罐成型工艺和成型模具设计要求;成型模具结构设计满足零件尺寸精度、脱模以及零件批量生产要求;碳纤维喇叭天线表面金属化达到了金属层致密、均匀、光顺以及导电率指标。通过以上工艺过程研究,制造出满足指标要求的碳纤维喇叭天线,可为此类碳纤维喇叭天线制造提供工艺借鉴。     

大振幅比超声变幅杆的优化设计

以提高超声加工中的材料去除率为目的,基于ANSYS有限元模态分析、谐响应分析模块,利用ANSYS优化设计方法对变幅杆的形状与谐振长度进行优化,完成了在满足许用应力条件下具有大振幅比的新型变幅杆设计.     

Pattern squint elimination for quad‐ridged conical and pyramidal horn antennas using bended probes

This article describes a new technique for pattern squint elimination of quad‐ridged conical and pyramidal horn antennas by introducing bended coaxial probes. Because of the phase difference and mutual coupling between vertical and horizontal polarizations, the radiation patterns of the conventional quad‐ridged conical and pyramidal horn antennas squint over a wide bandwidth. The proposed technique substantially reduces the phase difference and coupling between the two probes, so a significant improvement in the radiation patterns over the frequency band of 8–18 GHz can be achieved. The designed modified horn antennas are most suitable as a feed element in reflectors of radar systems and EMC applications. The proposed modified antennas have a voltage standing wave ratio (VSWR) less than 2.2 for the frequency range of 8–18 GHz. Moreover, the proposed antennas exhibit high gain, dual‐polarization performance, good isolation, low SLL, low back lobe, low cross polarization, and satisfactory far‐field radiation characteristics for the entire frequency band. © 2009 Wiley Periodicals, Inc. Int J RF and Microwave CAE 2010.     

Texture analysis of poly-adenylated mRNA staining following global brain ischemia and reperfusion

Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1 h of reperfusion after 10 min of normothermic global cerebral ischemia. At 1 h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.     

气象预警管理系统在宁夏农村防灾减灾中的应用

以LED电子屏和语音大喇叭的气象预警管理系统在宁夏农村防灾减灾建设中的研究为背景,介绍该系统的结构、功能和特点,以及使用中存在的问题和对策等.该气象预警管理系统基于C/S架构,结合了LED气象预警电子屏终端和语音大喇叭,主要实现面向LED电子屏及大喇叭的气象预报、预警信息编辑、录入、审核、发布与GPRS终端管理等功能,为宁夏农村气象信息服务和防灾减灾服务提供安全、快捷、有效的发布管理平台.     

毫米波宽带四加脊圆锥喇叭天线研究

本文利用有限单元法对毫米波宽带四加脊圆锥喇叭天线进行了理论分析和综合优化设计。对于这种形式的毫米波宽带天线在频率范围26—40G内的驻波分布情况进行了重点研究。并且，根据设计尺寸制作了一个实验样品，完成了有关实验，获得了满意的实验结果。     

磁介质涂层圆锥喇叭天线的设计方法

通过在圆锥喇叭内壁加磁介导涂层的方法，成功地设计了具有低副瓣、低交叉极化和方向图对称的喇叭天线。该磁介质涂层既是良导电体，又是良导磁体。通过选择合适的喇叭张角，该喇叭天线与波纹喇叭性能相同，而且比波纹喇叭设计简单。