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排序方式: 共有3108条查询结果,搜索用时 15 毫秒
1.
Isothermal nucleic-acid amplification methods such as Loop-Mediated isothermal AMPlification (LAMP) are increasingly appealing alternatives to PCR for use in portable diagnostic system due to the low cost, weight, and power requirements of the instrumentation. As such, interest in developing new probes and other functionality based on the LAMP reaction has been intense. Here, we report on the development of duplexed LAMP assays for pathogen detection using spectrally unique Assimilating Probes. As proof of principle, we used a reaction for Salmonella enterica as a model coupled with a reaction for λ-phage DNA as an internal control, as well as a duplexed assay to sub-type specific quarantine strains of the bacterial wilt pathogen Ralstonia solanacearum. Detection limits for bacterial DNA analyzed in individual reactions was less than 100 genomic equivalents in all cases, and increased by one to two orders of magnitude when reactions were coupled in duplexed formats. Even so, due to the more robust activity of newly available strand-displacing polymerases, the duplexed assays reported here were more powerful than analogous individual reactions reported only a few years ago, and represent a significant advance for incorporation of internal controls to validate assay results in the field. 相似文献
2.
PKI/CA技术综述 总被引:6,自引:0,他引:6
公开密钥基础设施(PKI)是解决网络安全的技术.典型PKI应用系统包括安全Web、注册机构(RA)、认证机构(CA)、目录(LDAP)和数据库等服务器.RA验证客户证书申请后提交CA.CA检查信息完整和数字签名正确后把证书存放到证书库和目录服务器,并将签发证书序列号通知客户和RA.客户即可使用该号通过目录服务器下载证书.1993年以来,美、加、欧洲、韩、日等国已相继展开PKI研究.我国2001年将PKI列入十五863计划,并着手解决PKI标准化、CA互联互通等关键技术问题。 相似文献
3.
Adriana Brancaccio Giovanni Leone Rocco Pierri 《AEUE-International Journal of Electronics and Communications》2004,58(6):382-390
In this paper, by applying a non linear model for the electromagnetic inverse scattering, a technique for the dielectric profiling of a planarly layered medium is investigated and applied to void localization and diagnostics inside a homogeneous lossless slab (one-dimensional geometry). Data are collected under plane wave multifrequency normal incidence. Suitable finite dimensional representations for the unknown functions are introduced and their influence on the model is discussed. The resulting functional equation is solved by the method of weighted residuals and the solution algorithm amounts to minimizing a non quadratic function, where particular attention is devoted to reduce the occurrence of local minima. Finally, the inversion algorithm is validated by applications to both simulated and experimental data. 相似文献
4.
Chih-Wea Wang Chi-Feng Wu Jin-Fu Li Cheng-Wen Wu Tony Teng Kevin Chiu Hsiao-Ping Lin 《Journal of Electronic Testing》2002,18(6):637-647
In this paper we propose a novel built-in self-test (BIST) design for embedded SRAM cores. Our contribution includes a compact and efficient BIST circuit with diagnosis support and an automatic diagnostic system. The diagnosis module of our BIST circuit can capture the error syndromes as well as fault locations for the purposes of repair and fault/failure analysis. In addition, our design provides programmability for custom March algorithms with lower hardware cost. The combination of the on-line programming mode and diagnostic system dramatically reduces the effort in design debugging and yield enhancement. We have designed and implemented test chips with our BIST design. Experimental results show that the area overhead of the proposed BIST design is only 2.4% for a 128 KB SRAM, and 0.65% for a 2 MB one. 相似文献
5.
A. Aryshev S. Araki P. Karataev T. Naito N. Terunuma J. Urakawa 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2007,580(3):1544-1551
A fast room temperature microwave detection system based on the Schottky Barrier-diode detector was created at the KEK ATF (Accelerator Test Facility). It was tested using Coherent Synchrotron Radiation (CSR) generated by the 1.28 GeV electron beam in the damping ring. The speed performance of the detection system was checked by observing the CSR from a multi-bunch (2.8 ns bunch separation time) beam. The theoretical estimations of CSR power yield from an edge of bending magnet as well as new injection tuning method are presented. A very high sensitivity of CSR power yield to the longitudinal electron distribution in a bunch is discussed. 相似文献
6.
Fujihiko Sakao 《Sadhana》1993,18(3-4):369-385
The sound generated aerodynamically by a pillow-like body simulating an obstacle in the human airway is observed through a
soft wall from outside the duct. It is intended to provide a physical basis for an attempted medical diagnostic technique
for detecting an obstacle by observing the sound during breathing. So far at least two kinds of sound have been identified:
One is sound of the half-jet flow formed downstream of the obstacle, and the other is sound due to unsteady motion of the
separation point on the curved surface of the obstacle. For simulating the flow condition downstream of the vocal cords during
inhalation, cases with an obstacle at various positions relative to a jet flow are also examined. In this case, the sound
due to the downstream obstacle exhibits a complicated dependence on the geometry and flow parameters. Nevertheless, increase
in the sound, or more correctly, deformation in the sound power spectrum, due to the presence of an obstacle is detectable
from outside, opening up possibilities of its use as a diagnostic aid.
Dedicated to the late Professor Itiro Tani on the occasion of 5ACFM, Taejon, Korea 相似文献
7.
A vibration-acoustic method for diagnostics and monitoring of production processes such as cutting metals makes it possible
compared with other methods to reduce the requirement for measuring equipment and it provides a computer representation of
the results observed. The set of equipment suggested has low cost and it exhibits considerable flexibility compared with existing
equipment.
__________
Translated from Izmeritel’naya Tekhnika, No. 7, pp. 30–34, July, 2006. 相似文献
8.
激光光束质量诊断技术的进展 总被引:1,自引:0,他引:1
本文介绍了激光光束质量诊断技术近年来的进展,以及国际标准化组织推荐的聚集光束测量法,指出了该技术的发展方向。 相似文献
9.
Optical measurements of thermal diffusivity of a material 总被引:2,自引:0,他引:2
The measurement of thermal diffusivity of a material (in particular, a thin film) is important for various reasons, e.g., to predict the heat transfer in the solid subjected to a thermal process, to monitor surface composition or morphology, or to detect invisible subsurface defects like delaminations. This measurement can be done in a noncontact manner using various photothermal methods. Such methods typically involve pulsed heating of the surface by small amounts using a laser source; the decay of the surface temperature after this pulsed photothermal heating is then probed to provide the thermal diffusivity. Various probing methods have been developed in the literature, including the probing of reflection, refraction, and diffraction from the pulsed heated area, infrared thermal radiometry, and surface deformation. This paper provides an overview of such techniques and some examples of their applications. 相似文献
10.
For the past decade, the Taiwanese government has applied the policy of “North heavy, South light” to put more emphasis on development in northern Taiwan instead of the south. This has resulted in uneven development between the northern and southern regions, especially when introducing, developing, and supporting high technology and resource allocation in education. Industry clusters have become the centerpiece of economic development policy in many parts of the world. In this study, it is assumed that there are two categories of less-advantaged regions in Taiwan: (1) older industrialized regions dominated by labor-intensive/capital-intensive industries, and (2) industrial regions that have merged with potential high-technology small firms but still lack infrastructure. This study discusses the following points: (a) How do industrial clusters work in action? (b) What barriers do less-advantaged industrial clusters face? (c) What action strategies promote less-advantaged industrial clusters? 相似文献