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1.
Dr. Adarshi P. Welegedara Dr. Ansis Maleckis Ruchira Bandara Dr. Mithun C. Mahawaththa Iresha Dilhani Herath Yi Jiun Tan Dr. Angeliki Giannoulis Prof. Daniella Goldfarb Prof. Gottfried Otting Prof. Thomas Huber 《Chembiochem : a European journal of chemical biology》2021,22(8):1480-1486
The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. GdIII−GdIII distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-GdIII were indistinguishable from GdIII−GdIII distances measured of MBP containing cysteine reacted with 4Br-MDPA tags. 相似文献
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Li Wang ping Mechanics Department Huazhong University of Science Technology Wuhan P.R.China Yeo K.S. Khoo B.C. Mechanical Production Engineering Department National University of Singapore 《水动力学研究与进展(B辑)》1999,(3)
1. INTRODUCTIONThemaingoalofthispaperistoexplorethepossibilitytolearnmoreaboutthemechanismofturbulentboundarylayerflowinteractionsanditseffectsoncompliantwallperformance.Therearecertainprerequisiteconditionstofurtherthestudyonthemechanism,i.e.theco… 相似文献
4.
对常规测井和核磁共振测井的渗透率公式进行了比较 ,发现各渗透率公式存在相互联系。几种核磁共振测井渗透率公式的计算结果和实验室渗透率的对比表明核磁共振参数和渗透率有很好的相关性 ;改进的渗透率公式可以准确地求取岩心和地层的渗透率。 相似文献
5.
Cif2000平台下的核磁共振测井解谱方法研究 总被引:3,自引:0,他引:3
介绍了在Cif2000多井解释平台下的核磁共振解谱方法与编程实现。解谱采用加入平滑因子后在特征矩阵的奇异值分解中截去小的非零奇异值的方法,可以在低信噪比时得到稳定的弛豫谱,在油田实际应用中证明了该方法的有效性。根据该方法在Cif2000平台上编制了完整的解谱处理程序,可以直接用于油田的生产实际。 相似文献
6.
Vatroslav Bubalo Vjekoslav Jerolimov Ivo Bau
i Mladen Andreis Zorica Veksli 《Polymer International》2005,54(5):848-853
The electron‐spin‐resonance (ESR) spin‐probe method, was used to study the heterogeneity of denture resins based on poly(methyl methacrylate). Results for three resins processed by microwave energy, conventional curing and cold curing (depending on the curing procedure and exposed to ageing in various environmental conditions) were compared. All three cured resins were stored over the same time (1200 h) in distilled water at ambient temperature and in artificial saliva at 348 K. The temperature‐dependent ESR spectra of a spin probe dispersed in the denture resins are analyzed in terms of line‐shapes and line‐widths. The appearance of two spectral components was taken as an indication of resin heterogeneity. The results reveal that the cold‐cured resin has a lower local density in comparison with microwave and conventionally cured resin. The amount of residual monomer also contributes to the local motion of polymer segments. The change of denture resins exposed to ageing is influenced both by the structure of the original resin and the ageing conditions. Restricted motion of a spin probe incorporated into the acrylic resins exposed to accelerated ageing suggests additional crosslinking of polymer chains. The differences are observed for all the investigated resins, but the highest change is observed with the cold‐cured resin. The ESR results are accompanied by Tg and Tm measurements. Copyright © 2005 Society of Chemical Industry 相似文献
7.
提出一种配电网电容电流测量的新方法,在零序电压互感器的开口三角侧串联一个可调电感,通过注入一个变频恒流信号寻找配电网的谐振频率,改变可调电感的数值后寻找另一谐振频率,联立2个谐振方程求解电容电流,该方法消除了电压互感器漏抗对测量的影响。开发了配电网电容电流测量仪,经模拟试验和现场测试表明,该方法具有安全、快捷、准确等特点,适用于中性点不接地或经随调式消弧线圈接地配电网。 相似文献
8.
A. K. Srivastava Ram M. Shrestha S. C. Srivastava Rabin Shrestha Dharam Paul 《国际能源研究杂志》2003,27(7):671-685
The power sector in India at present comprises of five separate regional electricity grids having practically no integrated operation in between them. This study analyses the utility planning, environmental and economical effects of integrated power sector development at the national level in which the regional electric grids are developed and operated as one integrated system. It also examines the effects of selected CO2 emission reduction targets in the power sector and the role of renewable power generation technologies in India. The study shows that the integrated development and operation of the power system at the national level would reduce the total cost including fuel cost by 4912 million $, total capacity addition by 2784 MW, while the emission of CO2, SO2 and NOx would be reduced by 231.6 (1.9%), 0.8 (0.9%), 0.4 (1.2%) million tons, respectively, during the planning horizon. Furthermore, the study shows that the expected unserved energy, one of the indices of generation system reliability, would decrease to 26 GWh under integrated national power system from 5158 GWh. As different levels of CO2 emission reduction targets were imposed, there is a switching of generation from conventional coal plants to gas fired plants, clean coal technologies and nuclear based plants. As a result the capacity expansion cost has increased. It was found that wind power plant is most attractive and economical in the Indian perspective among the renewable options considered (Solar, wind and biomass). Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
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10.
A. Alt Dr.‐Ing. H. Mertens U. Arz L. Blessing C. Berger 《Materialwissenschaft und Werkstofftechnik》2007,38(5):402-409
High cycle fatigue of bolted connections Extensive tests regarding the influences on the fatigue of bolt‐nut‐connections of preloading with torsion, of preloading with yielding, of loading with superimposed bending and of the tested lot are processed. These influences are not yet known according to VDI 2230. New testing devices were designed for these tests, which allow a far less expensive operation and may easily be used for bolts of diameters up to M100 and testing frequencies up to 1000 Hz. The validity of fatigue resistance according to VDI 2230 is specified with respect to the test results. The determined influence of the tested lots is unexpectedly high. The manufacturing process of bolts should be improved to minimize this influence. 相似文献