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Zhen-Yu Chen Ya-Lun Su Yu-Rong Bi Suk Ying Tsang Yu Huang 《Journal of the American Oil Chemists' Society》2000,77(1):73-78
There is an increasing interest in natural antioxidants present in traditional Chinese herbal medicines. The present study
examined the antioxidant activity of heane, acetone, and methanol extracts, as well as baicalein purified from the dry roots
of Scutellaria baicalensis Georgi (common name: Huangqin), in heated canola oil. Oxygen consumption and decreases in linoleic acid linolenic acid content
were monitored in canola oil held at 90–93°C. Among the three extracts, the acetone extract was most effective against oxidation
of canola oil, followed by the methanol extract of the dry roots. The antioxidant activity of these three extracts correlated
well with their content of baicalein, which provided strong protection to canola oil from oxidation. The antioxidant activity
of Huangqin acetone extract was dose-dependent. The acetone extract at 100 ppm or above was even more effective than butylated
hydroxytoluene at 200 ppm in protecting canola oil from oxidation. The present results suggest that the acetone extract of
these roots should be further explored as a potential source of natural antioxidants for use in the processed foods. 相似文献
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目的: 探讨黄芩苷对Bcap-37 细胞生长及TIMP2 表达的影响。方法: 体外细胞培养、MTT 法测定不同浓度黄芩苷对Bcap-37 细胞生长曲线的影响, RT-PCR 、Western blot 方法研究不同浓度黄芩苷对Bcap-37 细胞TIMP2 的影响。结果: 黄芩苷对Bcap-37 细胞的生长抑制呈浓度依赖关系;黄芩苷对Bcap-37 细胞TIMP2 在mRNA 水平、蛋白水平呈浓度依赖关系, 小剂量上调, 高剂量下调。结论: 黄芩苷对人乳腺癌Bcap-37 细胞生长有抑制作用, 可能与肿瘤细胞TIMP2 的下调有关。 相似文献
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目的 对黄芩叶进行系统毒理学研究得到安全性基础数据。方法 以黄芩叶提取物为受试物,采用食品安全国家标准方法,对黄芩叶开展急性经口毒性试验、细菌回复突变试验、哺乳动物红细胞微核试验、小鼠精母细胞染色体畸变试验以及90 d经口毒性试验,进行系统的毒理学安全性评价。结果 黄芩叶提取物急性经口毒性试验雌雄小鼠LD50>20.0 g/kg?BW,雌雄大鼠LD50>15.0 g/kg?BW,属于实际无毒级;细菌回复突变试验、哺乳动物红细胞微核试验及小鼠精母细胞染色体畸变试验3项遗传毒性检测结果均为阴性;90 d经口毒性试验中,2.0、4.0、8.0 g/kg?BW 3个剂量组大鼠的体质量、进食量、食物利用率、血液学、血生化等指标,与对照组比较无显著性差异。结论 在本试验剂量条件下,未发现黄芩叶提取物摄入对实验动物产生毒性作用,换算后黄芩叶NOAEL值为24.0 g/kg?BW。 相似文献
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考察了水煎煮法、乙醇回流法、乙醇室温浸提法对黄芩总黄酮得率和纯度的影响。确定乙醇室温浸提法的工艺条件为:50%乙醇室温浸提3次,每次24h;酸沉温度40℃;酸沉pH为0.5。该工艺稳定性好,黄芩总黄酮的平均得率为7.68%。 相似文献
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