深部松软煤层钻孔孔周煤体变形产渣特征研究

针对煤矿井下高瓦斯软煤顺层长钻孔排渣困难、成孔率低、施工困难等问题,通过数值模拟实验研究了井下深部软煤体变形破坏特征,分析了顺层长钻孔孔周松软煤体变形特征及应力变化,以揭示顺层长钻孔孔周松软煤体变形产渣规律。研究表明:深部高瓦斯软煤顺层钻孔孔周煤体的应力平衡临界条件破坏后将发生大体积突然垮落;钻孔水平最大变形位移为1.22mm,垂直方向最大变形位移为10.7mm;径向孔周煤体垂向变形呈现逐渐减小趋势,且垂向变形明显大于钻孔水平变形。在水平方向上,钻孔孔周煤体应力分布呈现先增大再逐渐减小的变化规律,径向距离对水平应力分布的影响逐渐减小;随着径向距离的增加,钻孔孔周煤体应力分布逐渐降低,钻孔孔壁处煤体的应力出现最大值,且垂直方向处应力值最大。     

Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

Monoclonal and recombinant antibodies are widely used for the diagnostics and therapy of cancer. They are generated to interact with cell surface proteins which are usually involved in the development and progression of cancer. Carbonic anhydrase XII (CA XII) contributes to the survival of tumors under hypoxic conditions thus is considered a candidate target for antibody-based therapy. In this study, we have generated a novel collection of monoclonal antibodies (MAbs) against the recombinant extracellular domain of CA XII produced in HEK-293 cells. Eighteen out of 24 MAbs were reactive with cellular CA XII on the surface of live kidney and lung cancer cells as determined by flow cytometry. One MAb 14D6 also inhibited the enzymatic activity of recombinant CA XII as measured by the stopped-flow assay. MAb 14D6 showed the migrastatic effect on human lung carcinoma A549 and renal carcinoma A498 cell lines in a ‘wound healing’ assay. It did not reduce the growth of multicellular lung and renal cancer spheroids but reduced the cell viability by the ATP Bioluminescence assay. Epitope mapping revealed the surface-exposed amino acid sequence (35-FGPDGENS-42) close to the catalytic center of CA XII recognized by the MAb 14D6. The variable regions of the heavy and light chains of MAb 14D6 were sequenced and their complementarity-determining regions were defined. The obtained variable sequences were used to generate recombinant antibodies in two formats: single-chain fragment variable (scFv) expressed in E. coli and scFv fused to human IgG1 Fc fragment (scFv-Fc) expressed in Chinese Hamster Ovary (CHO) cells. Both recombinant antibodies maintained the same specificity for CA XII as the parental MAb 14D6. The novel antibodies may represent promising tools for CA XII-related cancer research and immunotherapy.     

展成式精密电火花加工机床

研制了一种展成式精密电火花加工机床，它含有3个回转机构和4个直线移动机构，工件苡轴，工具电极苡轴和转台均可按需要回转，坐标工作台可作纵向和横向移动，工件头架可作轴向和竖向移动。可设置成多种复合运动方式和相应的结构布局，具有很丰富的加工功能，加工精度能达到微米级并实现电火花镜面加工。     

三峡地下电站变顶高尾水洞水动力特性

在国内首次应用模型水轮发电机组对三峡地下电站变顶高尾水洞方案进行水力过渡过程试验研究。对水轮机导叶实行分段关闭 ,并求出了二段关闭的最佳拐点 ,试验证实了二段关闭对减小尾水管进口的负压和机组转速上升有明显的作用。通过有关水力参数的测试 ,认为变顶高尾水洞可以满足各项技术要求 ,是一个减少山体开挖、节省投资的新型设计方案。在地下电站中具有广泛的应用前景     

FUZZY控制规则的自生成及其自校正方法

本文研究了:1)以被控对象规则模型为依据自动生成Fuzzy控制器规则的新方法;2) 其相应的自校正Fuzzy控制方法.最后以一实验室电加热炉温控实例说明它们的可行性和 有效性.     

Survivability of systems under multiple factor impact

The paper considers vulnerable multi-state series-parallel systems operating under influence of external impacts. Both the external impacts and internal failures affect system survivability, which is determined as the probability of meeting a given demand. The external impacts are characterized by several destructive factors affecting the system or its parts simultaneously.In order to increase the system's survivability a multilevel protection against the destructive factors can be applied to its subsystems. In such systems, the protected subsystems can be destroyed only if all of the levels of their protection are destroyed.The paper presents an algorithm for evaluating the survivability of series-parallel systems with arbitrary configuration of multilevel protection against multiple destructive factor impacts. The algorithm is based on a composition of Boolean and the Universal Generating Function techniques.Illustrative examples are presented.     

贯流式水轮发电机集电环短路原因分析及改善措施

白石窑水电厂1～4号发电机组在投运初期,集电环曾多次出现绝缘降低甚至短路的非正常现象。通过分析,引发故障的原因主要是碳刷架、集电环绝缘结构设计欠妥、运行环境恶劣以及机组的振动过大。针对上述原因,改进了绝缘结构设计上的不足,加强巡视和维护检查,装设工业电视监控,加装碳粉回收装置,对受油器进行防漏油或喷油技术处理,加强清污力度、提高发电水头以便减少机组振动,从内外因加以防范和改善后,问题得到了标本兼治,取得了理想的效果。     

移动探针测试技术的运用及测试结果剖析

主要讨论印刷电路板(PCB)移动探针测试中软件及检测中遇到特殊情况的处理,并对造成通短路现象的原因进行了分析,以达到保证PCB产品质量的目的。     

一种新型声波降粘防蜡装置

新型声波降粘防措装置由上、下接头、振动簧片和收缩喷嘴构成。装置安装在抽油泵泵简之下，尾管之上，在压差作用下井下流体以较大速度从装置的喷嘴射出，冲击振动簧片，产生机械振动声波，油水混合液在声波中产生相应的声波力学效应和分散乳化作用等，使混合液在油管内的粘度降低，蜡晶不易聚集长大，从而达到降粘防蜡延长油井热洗周期的目的。室内试验及现场近20口井的应用情况表明，这种装置具有结构简单、成本低廉、应用方便等特点，具有明显的降粘防蜡效果。     

安居水电厂灯泡贯流式水轮发电机组的试运行

本文对安居水电厂2台灯泡贯流式水轮发电机组自投入试运行以来,出现的受油器壳体开裂、导叶接力器缸体和活塞拉伤、转轮桨叶传动拐臂连接销脱落、导叶外轴承座漏水、主轴工作密封漏水侵入水导轴承、锁锭装置固定螺钉剪断等故障的经过、原因分析及其处理办法进行了叙述。