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排序方式: 共有1807条查询结果,搜索用时 15 毫秒
1.
Bernette Maria Oosterlaken Heiner Friedrich Gijsbertus de With 《Microscopy research and technique》2022,85(1):412-417
Transmission electron microscopy (TEM) is an important analysis technique to visualize (bio)macromolecules and their assemblies, including collagen fibers. Many protocols for TEM sample preparation of collagen involve one or more washing steps to remove excess salts from the dispersion that could hamper analysis when dried on a TEM grid. Such protocols are not standardized and washing times as well as washing solvents vary from procedure to procedure, with each research group typically having their own protocol. Here, we investigate the influence of washing with water, ethanol, but also methanol and 2-propanol, for both mineralized and unmineralized collagen samples via a protocol based on centrifugation. Washing with water maintains the hydrated collagen structure and the characteristic banding pattern can be clearly observed. Conversely, washing with ethanol results in dehydration of the fibrils, often leading to aggregation of the fibers and a less obvious banding pattern, already within 1 min of ethanol exposure. As we show, this process is fully reversible. Similar observations were made for methanol and propanol. Based on these results, a standardized washing protocol for collagenous samples is proposed. 相似文献
2.
介绍了观音阁坝被埋水下6年越冬停浇面水平裂缝采用“粘贴T1密封胶及表面粘三元乙丙后锚固”的处理方案。结果表明,利用胶性材料处理运行中的重力坝迎水面裂缝,技术可靠,方法简单,经济合理。 相似文献
3.
Fernando J Beltrn F Javier Rivas Olga Gimeno 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2005,80(9):973-984
Photocatalytic ozonation (1O3 + VUV + TiO2), ozonation (O3), catalytic ozonation (O3 + TiO2), ozone photolysis (O3 + VUV), photocatalysis (TiO2 + VUV) and photolysis (VUV) have been compared in terms of formation of intermediates, extent of, mineralization (TOC, COD, chloride, nitrate) and kinetics in the aqueous treatment of three phenols (phenol, p‐chlorophenol and p‐nitrophenol). In all cases, photocatalytic ozonation led to lower degradation times for chemical oxygen demand and total organic carbon removal. Intermediates formed were similar in the different oxidation systems with some exceptions. They can be classified into three different types: polyphenols (resorcinol, catechol, hydroquinone), unsaturated carboxylic acids (maleic and fumaric acids) and saturated carboxylic acids (glyoxylic, formic and oxalic acids). First order kinetic equations have been checked for the oxidation processes studied in the case of the parent compound. Rate constants of these systems have also been calculated. Copyright © 2005 Society of Chemical Industry 相似文献
4.
1976年汛前发现响水潭水库输水管竖井圆筒底部与闸室连接处的下游出现环形水平裂缝,对此当年曾加固处理。1986年复查时,发现竖井原处又出现了类似的环形裂缝,并有新的发展,为确保工程安全,于1994年3月再次加固。此次加固采用先止水,后粘钢板的加固方法,收到很好的效果。 相似文献
5.
溶胶-凝胶生物玻璃多孔材料显微结构和生物活性的扫描电镜及红外光谱分析 总被引:13,自引:0,他引:13
本文利用溶胶.凝胶法制备了CaO-P2O5-SiO,系统生物活性玻璃,并以其为原料制备了用于骨修复及骨组织工程支架的块状生物活性材料。利用体外实验方法(invitro)结合x射线衍射(XRD)、扫描电子显微镜(SEM)和傅立叶变换红外光谱分析(FTIR)技术对不同烧结工艺制备的材料结构、晶相和生物活性的影响进行了分析研究。研究表明,经800℃和1100℃烧结5min的两种材料均有硅磷酸钙Ca5(PO4)2SjO4微晶相析出。烧结温度越高,析出微晶相的体积比例越大,材料的生物活性则越低。在模拟生理溶液中碳酸羟基磷灰石(HCA)只在烧结体中的玻璃颗粒表面形成,而在其硅磷酸钙Ca5(PO4)2SiO4微晶相表面未发现HCA形成。 相似文献
6.
We have studied the short term C mineralization of six wastes from important food industries, one sludge from a biogas plant and three composts. All the wastes were characterized chemically and fractionated according to the Van Soest method. The fresh wastes were incubated under controlled environment conditions to determine the C mineralization rate. Based on first order mineralization kinetics, we calculated the hypothetical amount of stable C in the wastes as the amount of C that would not be mineralized within one year under field conditions. The percentage of stable organic C in the organic matter was in general much larger in the composts than in the other wastes, but when expressed on dry matter, the non-composted wastes had comparable or larger amounts of stable organic C than the composts and have a considerable potential for supplying organic matter to soils, and hence for C sequestration. The amount of stable organic C could best be predicted by the total N content of the wastes (R
a
2 = 0.855), whereas the results of the fractionation had very little predictive power, probably due to problems related to the high ash content of some of the wastes. An index that combined stable organic C and N and P content in the wastes was calculated to assess possible limitations for applying these wastes in agriculture. Under current nutrient legislation in Western Europe, a number of these wastes will only be usable in small amounts, but these and other food industry wastes could still prove to be valuable soil amendments in nutrient poor situations, for increasing soil organic C content and supplying nutrients. 相似文献
7.
8.
地层微电阻率成像测井在中原油田的应用 总被引:2,自引:0,他引:2
介绍地层微电阻率测井(FMI)技术以及它在地质构造解释、沉积学解释、裂缝识别、地层分析等方面独特的枝术优势,并详细介绍了FMI在中原油田濮深X井的实际应用,进行了应用效果分析,指出成像测井是未来油田油气勘探开发的重要技术手段。 相似文献
9.
浅成热液金矿业已成为最重要的金矿类型之一本文探讨了浅成热液金矿成因研究方面若干基本问题:浅成热液金矿的概念和特征、矿床的分类、矿床成因模式。重新厘定了浅成热液金矿的概念.尝试性地按成矿深度和成矿作用方式—斌矿地质环境(容矿岩系)—成矿物理化学条件的分类原则, 将浅成热液金矿分成两大类四种类型:热水沉积岩型、热水火山岩型、浅成热液型和卡林型。前两者为热水沉积型,后两者为热液交代型。在透视现有各种成矿模式的建模依据和存在问题的基础上,指出了今后浅成热液金矿成因研究的发展方向。 相似文献
10.
基岩地球化学方法广泛应用于地质找矿与生产实践,是开展深部找矿预测最直接的方法和手段。通过对玲珑金矿田井下含矿断裂和招平断裂的基岩剖面地球化学特征进行系统研究,得出以下认识:玲珑金矿田原生晕最佳指示元素组合为Au、Ag、Bi、As和Co,且Au-Ag、Au-Bi、Au-As及Au-Co元素之间呈较好的幂指数正相关。根据玲珑金矿田已探明矿脉最佳指示元素组合的统计结果,结合招平断裂基岩剖面地球化学测量结果,发现招平断裂在地表有2处金矿化异常,推测其为矿致异常,反映出该地段具有较好的找矿潜力,特别是前花园村东1021剖面中异常找矿潜力更大。 相似文献