补骨脂素和异补骨脂素的分离纯化研究

应用高速逆流色谱技术分离和纯化补骨脂中香豆素类成分补骨脂素和异补骨脂素．以补骨脂干燥成熟果实为原料,经乙醇回流制备补骨脂粗提物,经高速逆流色谱分离,所得样品由高效液相色谱检测纯度,并由对照品确定成分．采用正己烷．乙酸乙酯-甲醇-水（7：3：5：5）作为两相溶剂体系,从补骨脂粗提物中分离得到补骨脂素和异补骨脂素,二者纯度分别达到99．4％,99．1％．该法可以成功用于补骨脂粗提物中补骨脂素和异补骨脂素的分离,并且具有制备量大和分离效率高的优点．     

超声-UPLC法测定补骨脂中补骨脂素和异补骨脂素

补骨脂是我国用于生产各种保健品的传统原料之一,其主要活性成分为补骨脂素和异补骨脂素。本实验改进中国药典补骨脂项下补骨脂素和异补骨脂素含量测定方法。采用C18ODS色谱柱(1.8μm,4.6 mm×50 mm),流动相乙腈-水(35∶65),流速0.5 mL/min,检测波长246 nm,测定药典法和超声法制备补骨脂供试品溶液中补骨脂素和异补骨脂素含量。5 min内补骨脂素、异补骨脂素分离良好。在相应浓度范围内2种化合物的线性关系良好,r>0.999 7;精密度RSD≤2%;平均加样回收率为>99%。超声法与索氏提取法相比无显著性差异(P>0.05)。该方法操作简便,节省时间和溶剂,为补骨脂中补骨脂素、异补骨脂素的分析提供了一种简便快速的新手段。     

Comparative metabolism of [3H]psoralen and [3H]isopsoralen by black swallowtail (Papilio polyxenes Fabr.) caterpillars

The comparative fate of tritiated preparations of a linear furanocoumarin (psoralen) and an angular furanocoumarin (isopsoralen) was determined in last-instar caterpillars of the black swallowtail butterfly (Papilio polyxenes Fabr.). Oral administration of either furanocoumarin at 5 g/g is followed by rapid metabolism, primarily through oxidative cleavage of the furan ring, and the metabolites are rapidly excreted. Isopsoralen is, however, metabolized at a somewhat slower rate than is psoralen, and levels of unmetabolized isopsoralen in body tissues of the treated caterpillars are about three-fold higher. These data are compatible with the hypothesis that a reduced detoxification rate accounts at least in part for the susceptibility ofP. polyxenes caterpillars to the deleterious effect of isopsoralens.     

Fate of photosensitizing furanocoumarins in tolerant and sensitive insects

The fate of [¹⁴C]xanthotoxin (8-methoxypsoralen) was studied in larvae of insect species that are tolerant (Papilio polyxenes Stoll) or sensitive (Spodoptera frugiperda J.E. Smith) to the phototoxic effects of photosensitizing psoralens. Both insects metabolize xanthotoxin by oxidative cleavage of the furan ring, but the detoxification occurs at a much more rapid rate inP. polyxenes in which >95% of an oral 5 g/g xanthotoxin dose is metabolized within 1.5 hr after treatment. The detoxification of psoralens byP. polyxenes appears to occur primarily in the midgut tissue prior to absorption, with the result that the intact phototoxin does not reach appreciable levels in body tissues. Studies with an angular furanocoumarin indicated that isopsoralens are metabolized byP. polyxenes at a somewhat slower rate than observed for psoralens; however, a reduced rate of metabolic detoxification of isopsoralens probably does not explain the fact that psoralen tolerance inP. polyxenes does not extend to the isopsoralen series.     

Psoralen and Isopsoralen’s Cloud-Point Extraction from Psoralea corylifolia L.

A novel separation method based on micelle-mediated extraction and cloud-point preconcentration was developed for the hydrophobic compounds of psoralen and isopsoralen from Psoralea corylifolia L. by high performance liquid chromatography with photodiode array detection. The GENAPOL X080 was chosen as the extract surfactant. A series of experimental conditions were probed to optimize the extraction and preconcentration process. The optimized technique is a cheap, simple, and sensitive method.