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排序方式: 共有768条查询结果,搜索用时 21 毫秒
1.
Rinji Akada Yoshirou Shimizu Yuji Matsushita Miho Kawahata Hisashi Hoshida Yoshinori Nishizawa 《Yeast (Chichester, England)》2002,19(1):17-28
Drug-resistance markers for yeast transformation are useful because they can be applied to strains without auxotrophic mutations. However, they are susceptible to technical difficulties, namely lower transformation efficiency and the appearance of drug-resistant mutants without the marker. To avoid these problems, we have constructed a phosphoglycerate kinase (PGK) promoter-driven YAP1 expression cassette, called PGKp-YAP1. Yeast cells containing PGKp-YAP1 were resistant to cycloheximide, a protein synthesis inhibitor, and also to cerulenin, a fatty acid synthesis inhibitor, but not to other drugs tested. The transformation efficiency of PGKp-YAP1 using cerulenin selection was comparable to that using a URA3 auxotrophic marker when low concentrations of cerulenin were used. Non-transformed drug-resistant colonies did appear on the low-concentration cerulenin plates. However, these non-transformed colonies could easily be identified, based on their cycloheximide sensitivity and/or their resistance to aureobasidin A to which the transformants were sensitive. Therefore, the dual drug resistance of PGKp-YAP1 could be used as an effective selection for PGKp-YAP1 recipient cells. The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain. 相似文献
2.
Su-Min Baek Seoung-Woo Lee Tae-Un Kim Seong-Kyoon Choi Sungho Yun Won-Jae Lee Se-Hyeon Han Il-Hwa Hong Sang-Joon Park Tae-Hwan Kim Kyu-Shik Jeong Jin-Kyu Park 《International journal of molecular sciences》2021,22(5)
Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species. 相似文献
3.
A comparative study of high-temperature oxidation of Ni containing 1 at.% Cr and pure Ni was carried out. Instead of the conventional kinetics study using thermogravimetry, a microlithographic marker experiment was designed. Observation of the markers using cross-sectional TEM and SEM has revealed striking differences in the scale morphology, microstructures, and oxidation mechanisms between pure Ni and the Cr-doped Ni substrates. In particular, the results suggest that a small addition of Cr promotes significant inward transport of oxygen. Marker experiments revealed that NiO grown on pure Ni is wholly attributable to outward-cation diffusion. In contrast, NiO grown on Ni–1 at.% Cr exhibited formation of a substantial inner layer having a submicron grain size, established by the markers to have formed from oxygen ingress. For pure Ni, voids were observed to be distributed only within oxide grains. In contrast, for Ni containing 1 at.% Cr, elongated pores formed extensively along oxide-grain boundaries. Formation of new fine-grain oxide in these pores was observed to have sometimes completely resealed the void. It is, therefore, proposed that the transport of oxygen in the case of oxide scale grown on Ni–1 at.% Cr occurs via voids (pores) formed by vacancy coalescence at the grain boundaries. 相似文献
4.
介绍了青霉的传统分类方法,综述了随机扩增多态性(RAPD)、限制性片断长度多态性(RFLP)、扩增片断长度多态性(AFLP)和rDNA/rRNA序列分析等几种分子标记技术的基本原理、方法及其青霉分类鉴定中的研究进展。 相似文献
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6.
话语标记的语体特征研究及应用 总被引:1,自引:0,他引:1
话语标记作为一种常见的话语现象,已成为话语分析研究的重要课题。由于研究角度不同,人们对于话语标记的认识和分类至今仍存在较大差异。该文从语体的角度提出假设,认为话语标记具有一定的语体特征。为准确描写话语标记的语体特征,提出了“语体度”的概念。通过对采样话语标记在不同语体的语料中分布情况进行定量分析,证实了相当一部分话语标记具有明显的语体特征,并根据分析结果选择特征向量,采用Rocchio分类法对开放文本进行自动语体分类实验,正确率达到82.9%。事实证明话语标记的语体特征对文本分类具有一定的参考价值。 相似文献
7.
近几十年来,尽管研究人员一直在努力开发新的诊断和治疗技术,癌症仍然是世界上发病率和死亡率最高的疾病之一。其中关键点是在肿瘤发生的早期进行诊断。现有的肿瘤早期诊断方法有很多缺点,比如对病患肿瘤组织的侵入。因此,与肿瘤相关的无创诊断的研究已经越来越多并已经取得进展。研究发现,蛋白、DNA或者RNA等生物大分子都有可能成为潜在的肿瘤标志物,已有的检测方法也存在许多缺点。纳米孔因具有独特的物理和电学性质,对生物分子的检测有快速、无需标记和扩增等优点,已被广泛使用和证明。在不远的将来,应用纳米孔对肿瘤标志物进行检测,进行肿瘤诊断并监控治疗过程值得期待。主要介绍了纳米孔传感技术应用于肿瘤早期诊断中肿瘤标志物检测的研究进展。 相似文献
8.
9.
一种基于分层率失真优化的容错性视频转码算法 总被引:1,自引:0,他引:1
容错性视频转码能够在已压缩视频流中嵌入容错性工具,以增强视频流面向无线信道的抗误码能力.但是容错性工具的嵌入在降低失真的同时也会增加码率,因此需要进行率失真优化.针对这一问题,文中提出了一种基于分层率失真优化的容错性转码算法.该算法通过在帧层和宏块层分别对重同步标记的插人和帧内宏块的刷新进行不同粒度的优化来提高算法的灵活性,通过在帧层考虑帧内宏块刷新、帧内预测和运动矢量预测的影响来提高算法的精确性,通过在宏块层减少候选模式的数量来降低算法的复杂度.实验表明,该算法与基于单层率失真优化的算法相比,信噪比可获得0.6~1.1dB的增益,复杂度最多可降低25%. 相似文献
10.
1.引言 二维流体力学计算方法按其采用Lagrange坐标系还是Euler坐标系,分为Lasrange方法和 Euler方法两大类. Lagrange坐标是随材料质团运动的,由于每个有限网格点总是与初始时同一部分流体相联系,所以拉氏程序能够清晰显示解域内部多种物质的分界向和自由界向,而且描述材料分界面的运动是自动完成的.然而在二维计算中,当材料发生大变形时,拉氏网格发生扭曲,甚至使相邻网格彼此相交和重叠,使计算过程无法进行下去.因此,Lagrange方法在二维计算中有其局限性,只能用了变形较小的模… 相似文献