Stem Photosynthesis—A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity

Grass pea (Lathyrus sativus) is a leguminous plant of outstanding tolerance to abiotic stress. The aim of the presented study was to describe the mechanism of grass pea (Lathyrus sativus L.) photosynthetic apparatus acclimatisation strategies to salinity stress. The seedlings were cultivated in a hydroponic system in media containing various concentrations of NaCl (0, 50, and 100 mM), imitating none, moderate, and severe salinity, respectively, for three weeks. In order to characterise the function and structure of the photosynthetic apparatus, Chl a fluorescence, gas exchange measurements, proteome analysis, and Fourier-transform infrared spectroscopy (FT-IR) analysis were done inter alia. Significant differences in the response of the leaf and stem photosynthetic apparatus to severe salt stress were observed. Leaves became the place of harmful ion (Na⁺) accumulation, and the efficiency of their carboxylation decreased sharply. In turn, in stems, the reconstruction of the photosynthetic apparatus (antenna and photosystem complexes) activated alternative electron transport pathways, leading to effective ATP synthesis, which is required for the efficient translocation of Na⁺ to leaves. These changes enabled efficient stem carboxylation and made them the main source of assimilates. The observed changes indicate the high plasticity of grass pea photosynthetic apparatus, providing an effective mechanism of tolerance to salinity stress.     

A miniature triaxial apparatus for investigating the micromechanics of granular soils with in situ X-ray micro-tomography scanning

The development of a miniature triaxial apparatus is presented. In conjunction with an X-ray micro-tomography (termed as X-ray μCT hereafter) facility and advanced image processing techniques, this apparatus can be used for in situ investigation of the micro-scale mechanical behavior of granular soils under shear. The apparatus allows for triaxial testing of a miniature dry sample with a size of \mathrm{8}\hspace{0.17em}\mathrm{mm}\times \mathrm{16}\hspace{0.17em}\mathrm{mm} (diameter \times height). In situ triaxial testing of a 0.4–0.8 mm Leighton Buzzard sand (LBS) under a constant confining pressure of 500 kPa is presented. The evolutions of local porosities (i.e., the porosities of regions associated with individual particles), particle kinematics (i.e., particle translation and particle rotation) of the sample during the shear are quantitatively studied using image processing and analysis techniques. Meanwhile, a novel method is presented to quantify the volumetric strain distribution of the sample based on the results of local porosities and particle tracking. It is found that the sample, with nearly homogenous initial local porosities, starts to exhibit obvious inhomogeneity of local porosities and localization of particle kinematics and volumetric strain around the peak of deviatoric stress. In the post-peak shear stage, large local porosities and volumetric dilation mainly occur in a localized band. The developed triaxial apparatus, in its combined use of X-ray μCT imaging techniques, is a powerful tool to investigate the micro-scale mechanical behavior of granular soils.     

GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation

Protein trafficking is altered when normal cells acquire a tumor phenotype. A key subcellular compartment in regulating protein trafficking is the Golgi apparatus, but its role in carcinogenesis is still not well defined. Golgi phosphoprotein 3 (GOLPH3), a peripheral membrane protein mostly localized at the trans-Golgi network, is overexpressed in several tumor types including glioblastoma multiforme (GBM), the most lethal primary brain tumor. Moreover, GOLPH3 is currently considered an oncoprotein, however its precise function in GBM is not fully understood. Here, we analyzed in T98G cells of GBM, which express high levels of epidermal growth factor receptor (EGFR), the effect of stable RNAi-mediated knockdown of GOLPH3. We found that silencing GOLPH3 caused a significant reduction in the proliferation of T98G cells and an unexpected increase in total EGFR levels, even at the cell surface, which was however less prone to ligand-induced autophosphorylation. Furthermore, silencing GOLPH3 decreased EGFR sialylation and fucosylation, which correlated with delayed ligand-induced EGFR downregulation and its accumulation at endo-lysosomal compartments. Finally, we found that EGF failed at promoting EGFR ubiquitylation when the levels of GOLPH3 were reduced. Altogether, our results show that GOLPH3 in T98G cells regulates the endocytic trafficking and activation of EGFR likely by affecting its extent of glycosylation and ubiquitylation.     

HART通信协议在汽油机速度测量和监控方面的应用

首先介绍了HART通信协议及他的优点，然后介绍了HART通信协议在汽油机测速方面的软件实现。该软件主要采用目前流行的VB编程语言。可设计出界面友好、功能强大的测量和监控软件。     

AOTF红外光谱测量系统及其设计分析

介绍了一种以声光可调谐滤光器(AOTF)为色散元件的新型红外光谱测量系统。系统采用计算机控制下的直接数字频率合成(DDS)技术，实现电调谐波长扫描。采用双光束相关检测技术，改善了系统的信噪比。文中对红外光谱测量系统的有关设计问题进行了论述。     

微桥法镍膜的弹性模量和残余应力测量

利用MEMS技术制作了不同尺寸的镍(Ni)膜微桥结构样品。采用纳米压痕仪XP系统测量了微桥载荷与位移的关系,并结合微桥力学理论模型得到了两种不同尺寸的Ni膜的弹性模量和残余应力。结果表明,两种不同尺寸的Ni膜的弹性模量结果一致,为190 GPa左右,但是残余应力变化较大。与采用纳米压痕仪直接测得的带有硅(Si)基底的Ni膜弹性模量186.8 7.5 GPa相比较,两者符合较好。     

铁路罐车密闭装车计量系统的运行分析

从中国石化股份公司济南分公司新上铁路罐车密闭装车计量系统的背景出发，阐述该系统的设计思想和组成；通过分析实际运行过程中仪表比对数据，讨论影响仪表贸易交接准确性的各种因素，提出相应的解决方法；最后通过铁路罐车密闭装车计量系统投用效果。证实该系统可产生明显的经济效益。     

一种新颖的MEMS光开关测量平台的设计与实现

随着光电器件的广泛应用，微机械光开关成为核心光交换器件的主流。在研发过程中，其器件检测手段成为人们所关注的话题。本文介绍了一种新颖的测量平台，通过高幅值利用单片机控制脉冲频率的方法来选择器件。与当前同类方法相比，具有精度高、可靠性强、成本低、易操作等特点，具有广阔的使用前景。     

弹道靶道测量精度检测方法之探讨

该文分析了弹道靶道测量中的精度问题及通常使用的静态检测方法,并针对静态检测与靶道实测相差甚远的问题,提出了一种动态检测方法.该方法充分考虑到弹道靶道的测量对象是高速飞行体以及照片质量、气流附面层、发射时的恶劣环境和振动等因素对测量精度的影响,结合误差理论,给出了动态精度的计算公式,在已建成的IB-12中间弹道靶道中应用,效果良好.     

AD2S80在测角系统中的应用

DS2S80是美国AD公司生产的以鉴幅方式对感应同步器信号进行处理的专用芯片。由它与感应同步器构成的测角系统具有成本低、功耗小、所需外部元件少、可靠性好等优点。介绍了AD2S80数字转换芯片的引脚、组成测角系统的工作原理、外围电路的设计，给出了一种半量式测角系统的实现方案。