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排序方式: 共有726条查询结果,搜索用时 15 毫秒
1.
Jie-Long He An-Te Chen Jyong-Huei Lee Shih-Kang Fan 《International journal of molecular sciences》2015,16(9):22319-22332
The basic structural and functional unit of a living organism is a single cell. To understand the variability and to improve the biomedical requirement of a single cell, its analysis has become a key technique in biological and biomedical research. With a physical boundary of microchannels and microstructures, single cells are efficiently captured and analyzed, whereas electric forces sort and position single cells. Various microfluidic techniques have been exploited to manipulate single cells through hydrodynamic and electric forces. Digital microfluidics (DMF), the manipulation of individual droplets holding minute reagents and cells of interest by electric forces, has received more attention recently. Because of ease of fabrication, compactness and prospective automation, DMF has become a powerful approach for biological application. We review recent developments of various microfluidic chips for analysis of a single cell and for efficient genetic screening. In addition, perspectives to develop analysis of single cells based on DMF and emerging functionality with high throughput are discussed. 相似文献
2.
一种PDMS薄膜型微阀的制备与性能分析 总被引:2,自引:0,他引:2
通过厚胶光刻工艺在硅片上制备SU-8胶模板,利用该模板制备了高分子聚合物PDMS(Polvdimethvlsiloxane,聚二甲基硅氧烷)微流道和薄膜结构。通过对不同结构的两层PDMS的不可逆粘接得到一种简单的阀结构,在外加气源压力作用下薄膜产生变形实现对微流道的控制。实验测量了微阀的控制气源压力与被控制液体流量之间的关系,说明膜阀的开闭性能良好。根据弹性薄膜的变形理论,对影响微阀性能的参数进行了分析,并提出了几种可行的用于薄膜微阀控制的方法。 相似文献
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提出了一种新的、基于声表面波的纸基微流开关。通过软光刻技术制作内含两个微孔的聚二甲基硅氧烷(PDMS)微架,其上固定经折叠、长度可变的纸通道。PDMS微架贴附于压电基片之上,并在待连接的两微通道之下方,折叠纸通道最低端离压电基片间距为2 mm。压电基片上采用微电子工艺光刻一对叉指换能器和反射栅。当足够强度的电信号加到叉指换能器对时,激发两相向声表面波,使得压电基片上微流体输运到折叠纸通道,改变其长度,连接其上待连通的两纸基微通道,完成开关功能。对可编程微流器件提供了一种新的编程和开关控制方法。 相似文献
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Lucian M. Rusnac Octavian Floarea Radu V. Vladea 《Journal of the American Oil Chemists' Society》1992,69(4):384-386
Continuous hydrogenation of industrially refined soybean oil with Harshaw Ni catalyst was achieved in a slurry column equipped
with Sulzer SMV motionless mixers. The influence of the operating parameters (temperature, pressure, catalyst concentration
and gas velocity) was investigated. The presumption that, in this equipment, the liquid-solid mass transfer limits the rate
of the process is in good agreement with the experimental data. 相似文献
9.
The ability to trap precise quantities of cells or particles into confined areas has numerous applications for biological purposes. In particular, single cell trapping has received considerable attention because it permits the study of heterogeneity in a population, while trapping larger groups of cells have been used to form aggregates. Among several methods, the use of microwell offers a simple platform to capture cells or particles using hydrodynamic forces. This review examines the use of microwells in both static and microfluidic environments, and the application of microfluidic geometric arrays for trapping. This paper discusses the design and fabrication methods of microwells and compares the trapping and release techniques used in both static and microfluidics‐integrated microwells. Finally, we will summarize novel microfluidic geometric arrays used to capture cells or particles through hydrodynamic trapping. 相似文献
10.
Peter E. Neerincx Martin Hofmann Oleksandr Gorodetskyi Kirill Feldman Jan Vermant Han E. H. Meijer 《Polymer Engineering and Science》2021,61(4):1257-1269
Relatively recently, we advanced a route to create, in a controlled fashion, combined horizontal and vertical stratified structures by simple and energy-efficient processing operations employing static mixing elements. While in state-of-the-art static mixing the focus is on layer multiplication, here the aim is to create hierarchical fractal structures. Therefore, the main question addressed in this article is how structures, rather than layers, can be multiplied. The key aspect is the addition of layers on the sides or in the midplane of the flow during the process; every addition step increases the hierarchy by one level. This article derives the general formalism for forming fractal structures with controlled hierarchy, and we develop the language required to design and construct the dies. The main part of the article addresses this main topic and is based on the splitting serpentine static mixer geometry that can be easily made on the parting surfaces of a mold on both the micro- and the macroscale. The second part of the article addresses the strategy to minimize the number of mirroring steps, eventually avoiding mirroring completely, and is based on the rotation-free multiflux static mixer geometry. With the design language derived, complex hierarchical fractal structures can be generated simply by changing the number and sequence of operators within extrusion dies or molds, providing a one-step solution to produce material structures for potential use in diverse applications ranging from advanced mechanical systems to photovoltaic devices, where controlled assembly of dissimilar materials, and the realization of huge interfaces and genuine cocontinuity throughout the cross section, is critical. 相似文献