A novel direct aerodynamically assisted threading methodology for generating biologically viable microthreads encapsulating living primary cells

In a recent discovery, coaxial electrospinning was explored to encapsulate living organisms within a continuous bio‐polymeric microthread from which active biological scaffolds were fabricated (Townsend‐Nicholson and Jayasinghe, Biomacromolecules 2006, 7, 3364). The cells were demonstrated to have gone through all expected cellular activity without their viability being compromised. These biologically active threads and scaffolds have direct and tremendous applicability from regenerative to therapeutic medicine. Currently these post‐processed cells as composite threads and scaffolds are being investigated in‐depth at a cellular level to establish if the processing methodology has any affect on the cellular make‐up. We now demonstrate a competing non‐electric field driven approach for fabricating composite threads and scaffolds influenced only by a differential pressure. We refer to this novel composite thread to scaffold fabrication methodology as coaxial aerodynamically assisted bio‐threading (CAABT). Our investigations firstly, demonstrate that this technique can process handle living organisms without biologically perturbing them in anyway. Secondly the process is elucidated as possessing the ability to form composite active threads from which biologically viable scaffolds are formed. Finally our study employs florescent activated cell sorting (FACScan), a method by which the cellular dynamics and viability are quantified on control and threaded cellular samples at two prescribed time points. In parallel with FACScan, optical comparison of cellular morphology at three time points within a period of three weeks is carried out to photographically observe any changes in the post‐processed cellular phenotype. Our developmental investigations into this novel aerodynamically assisted threading methodology has unearthed a unique biomicrofabrication approach, which joins cell electrospinning in the cell threading to scaffold fabrication endeavor. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

智能电网的安全可控性

计算机与信息技术在电力系统中的广泛应用提高了电网的自动化、智能化水平,同时也将传统IT领域的众多安全隐患引入了电网。智能电网是社会域、信息域、物理域多域交互、渗透形成的大规模新型融合网络,其安全威胁具有多域渗透、跨域攻击的特点。文中描述了智能电网基本安全需求及其与传统IT安全需求的不同;分析了智能电网中多域渗透攻击并且对信息-物理安全威胁进行分类;基于分域防护、多域协同、边界防护的思想提出了智能电网多域协同安全防护模型。     

多步自适应组合预测及其应用

提出一种多步自适应组合预测方法.预测由多个并行不同的多步自适应预测模型组成,最终预测结果,由多个预测模型预测值的线性加权组合决定.本法适用于随机时变动态系统过程的多步预测问题,在某省粮食产量趋势预测中,应用此法获得了良好的效果.     

基于企业核心竞争力的中部企业“多梯制”人才发展通道研究

从提高中部企业核心竞争力的角度出发,对中部企业人才发展通道不畅的现状和原因进行分析,提出建立管理人才、技术人才和技能人才“多梯制”发展通道的构想,试图通过充分发挥人才资源的作用来有效整合其内部和外部的各种资源,不断推陈出新,提升企业的核心竞争力,使中部企业在日益激烈的市场竞争中始终立于不败之地,实现中部地区的快速崛起。     

Genetic Threading

The biological function of proteins is dependent, to a large extent, on their native three dimensional conformation. Thus, it is important to know the structure of as many proteins as possible. Since experimental methods for structure determination are very tedious, there is a significant effort to calculate the structure of a protein from its linear sequence. Direct methods of calculating structure from sequence are not available yet. Thus, an indirect approach to predict the conformation of protein, called threading, is discussed. In this approach, known structures are used as constraints, to restrict the search for the native conformation. Threading requires finding good alignments between a sequence and a structure, which is a major computational challenge and a practical bottleneck in applying threading procedures. The Genetic Algorithm paradigm, an efficient search method that is based on evolutionary ideas, is used to perform sequence to structure alignments. A proper representation is discussed in which genetic operators can be effectively implemented. The algorithm performance is tested for a set of six sequence/structure pairs. The effects of changing operators and parameters are explored and analyzed.     

正交遗传算法在网络优化设计中的应用

利用正交实验法的全局思想,提出一种采用多点正交交换的遗传算法。算法通过正交表安排遗传算法的交换运算,并在所产生的多个子代中选择适应度大的个体进入下一代进化,这样既加快了算法的收敛速度又保证了种群的多样性。并将该算法应用在计算机网络的容量分配与路由选择优化上。实验证明,该算法较之传统遗传算法,在种群规模较小的情况下,仍然可以以较少的搜索次数,收敛到近似最优解。     

多舵机控制在可遥操作个人机器人上的应用

文章介绍的机器人的实现了GSM短信息远程控制技术和基于GPS的自主定位技术。机器人能够模仿人类的动作行为是机器人学的一个研究重点,本文提出了一种多舵机控制方法,实现了机器人学习并模仿人类的动作。通过语音识别和语音合成技术实现机器人和人自然和谐交互。     

基于多层分布式结构的用户供电可靠性综合管理信息系统

基于多层分布式结构的用户供电可靠性综合管理信息系统即将在全国供电企业推广使用。章给出了该综合管理信息系统的主要开发原则、所采用的网络结构和优点以及主要数据结构和模型，指出了其在指标计算和分析功能方面对以前版本所作的重点改进情况，并介绍了该系统的其他主要功能。     

天津钢铁有限公司视频方案探讨

介绍了视频服务器方式、硬盘录像机内嵌视频服务器方式、数字网络方式的特点，及天津钢铁有限公司视频系统的选型。     

基于数字地图的多目标路径优选方案的研究

最短路径分析是地理信息系统(GIS)中空间网络分析的关键内容之一．面对当前城市道路网、信息传输网中出现的道路拥挤、信息堵塞等问题，在利用一种改进的Dijkstra算法获得单目标最优路径的基础上，从解决路段冲突入手，提出了两种多目标路径优选的实现方案．实践表明，该方案有效可行．中还对算法实现过程中涉及到的基本概念和数据结构进行了介绍．