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1.
Angiotensin I‐converting enzyme inhibitory and Ca‐binding activities of peptides prepared from tuna cooking juice and spleen proteases
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Jirawadee Kasiwut Wirote Youravong Pittaya Adulyatham Nualpun Sirinupong 《International Journal of Food Science & Technology》2015,50(2):389-395
Tuna cooking juice is a by‐product from the tuna canning industry. In this study, tuna cooking juice was hydrolysed by proteases extracted from the spleen. Tuna cooking juice showed the highest ACE inhibitory and Ca‐binding activities after hydrolysis for 270 and 180 min, respectively. The hydrolysate was further fractionated by ultrafiltration. The permeate exhibited highest ACE inhibitory and Ca‐binding activities when passed through 1 and 5 kDa cut‐off membranes, respectively. Gel filtration chromatography was used to determine the MW of bioactive peptides that exhibited highest ACE inhibitory and Ca‐binding activities. Those peptides that exhibited highest ACE inhibitory and Ca‐binding activities were the MW range of 238–829 Da and 1355–1880 Da, respectively. These results suggest that the tuna cooking juice and the spleen protease extract are a potential source of bioactive peptides that can be utilised as bioactive ingredients in functional food and nutraceuticals. 相似文献
2.
Mri Miczi Mria Golda Balzs Kunkli Tibor Nagy Jzsef Tzsr Jnos Andrs Mtyn 《International journal of molecular sciences》2020,21(24)
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His6-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2. 相似文献
3.
Bioremediation by alkaline protease (AkP) from edible mushroom Termitomyces clypeatus: optimization approach based on statistical design and characterization for diverse applications
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4.
杨宁 《重庆理工大学学报(自然科学版)》2006,20(12):93-95,115
抗日战争胜利初期,国共内战迫在眉睫。为挽救危局,强国富民,中间人士提出所谓中间路线,企图走“第三条道路”实现和平建国。在反动政府的强力弹压和中共的教育帮助下,中间路线退出历史舞台。民主党派的转型历程充分昭示了中国人民在中共领导下走上新民主主义道路的必然性。 相似文献
5.
Tatsuro Kijima Kouji Ohshima Hideo Kise 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1994,59(1):61-65
Racemic amino acid esters were optically resolved via hydrolysis in organic solvents by the catalysis of an industrial alkaline protease, “Alcalase”. The products which were composed mainly of L-amino acids were insoluble and easily separated by filtration. The activity of the enzyme and enantiomeric excess of the products were significantly dependent on the nature of solvent and the water content in the reaction media. Generally, high values of enantiomeric excess were obtained at low water contents. Many natural and unnatural amino acids were resolved by this method. 相似文献
6.
Sangeeta A Godbole Thirumalai G Krishna Chittaranjan R Bhatia 《Journal of the science of food and agriculture》1994,64(3):331-335
Cajanus trypsin inhibitor (CTI) and Cajanus trypsin-chymotrypsin inhibitor (CTCI) previously purified from cv TAT-10 were further characterised. The modification of the inhibitors revealed the presence of lysine at the trypsin reactive site in both CTI and CTCI. Modification of tyrosine at the reactive site of CTCI did not abolish chymotrypsin inhibition suggesting the presence of leucine or phenylalanine as reported in other chymotrypsin inhibitors. CTCI did not contain tryptophan. Dissociation constants for the inhibitors with bovine trypsin were in the region of 0.69 nmol (CTCI) and 0.029 nmol (CTI). Although the protease inhibitors lost their inhibitory activity on exposure to 2-mercaptoethanol they remained attached to the enzyme. The inhibitors were not very effective against the protease from Helicoverpa armigera which is a serious field pest of Cajanus. Dissociation constants for the inhibitors with the larval enzyme were in the region of 100 nmol. 相似文献
7.
本文介绍了给水联合处理的原理及其优点以及在望亭电厂的1台300MW亚临界燃油直流炉上进行这项处理的工业性试验的过程、效果及存在问题。最后作者对给水联合处理能否取得成效的关键提出了意见。 相似文献
8.
本文考虑了单输人中立型时滞系统的镇定问题。利用Byrnes等人提出的中立型时滞系统的模型,我们证明:如果系统是非奇异的,则系统可镇定的充要条件是其谱可镇定。 相似文献
9.
Biological and technological systems process information by means of cascades of signals. Be they interacting genes, spiking neurons or electronic transistors, information travels across these systems, producing, for each set of external conditions, an appropriate response. In technology, circuits performing specific complex tasks are designed by humans. In biology, however, design has to be ruled out, confronting us with the question of how these systems could have arisen by accumulation of small changes. The key factor is the genotype-phenotype map. With the exception of RNA folding, not much is known about the exact nature of this mapping. Here, we show that structure of the genotype-phenotype map of simple feed-forward circuits is very close to the ones found in RNA; they have a large degree of neutrality, by which a circuit can be completely rewired keeping its input-output function intact, and there is a relatively small neighbourhood of a given circuit containing almost all the phenotypes. 相似文献
10.
An earlier neural network analysis of processing of steel plates through hot rolling was subjected to a further refined analysis through some flexible neural networks that evolved using a multi‐objective predator‐prey genetic algorithm. The original data set expressing the Yield Strength and Ultimate Tensile Strength of the rolled slabs in terms of a total of 108 process variables were subjected to a systematic pruning through this evolutionary approach, till the nitrogen content of the steel emerged as the most significant input variable. A theoretical explanation is provided for this slightly unexpected result. 相似文献