超声雾化萃取大气压光电离质谱法快速分析烟草化学成分

随着烟草工业的发展,对烟草样品进行快速、准确和高灵敏度地分析检测已经成为烟草化学发展的必然趋势和产品研发的现实需求.本工作利用超声雾化萃取大气压光电离质谱(EAPPI-MS)技术,无需样品预处理和色谱分离,对1R5F、3R4F、都宝、七星和王冠5种卷烟烟丝中的化学成分进行直接、快速分析,鉴别出醇、酮、酸、酯、醛、酚、生...     

Fast chromatography of pulse triacylglycerols

A short 10-min ultra high performance liquid chromatography (UHPLC) method was used for separation and lipidomic analysis of triacylglycerols (TAGs) in 10 pulses: baby lima beans, black beans, black-eyed peas, butter beans, cranberry beans, garbanzo beans, green split peas, lentils, navy beans, and pinto beans. The lipids extracted using chloroform/methanol averaged 1.9–2.7% across all pulses except garbanzo beans, which gave 6.2% lipids. TAGs were analyzed using dual parallel mass spectrometry (LC1MS2), areas were integrated using LipidSearch 4.2 software, and percent relative TAG compositions were calculated. Fatty acid response factors were calculated by comparison to calibrated GC-FID, which were used to calculate response-factor adjusted TAG compositions. Principal component analysis revealed that the pulses separated into three clusters, which were further highlighted using hierarchical cluster analysis. A subset of TAGs was quantified using calibration curves made from alternating sets of regioisomers in the non-linear high concentration range (~2.5 nMol/ml to ~300 nMol/ml). A linear calibration curve for the sum of tocopherols detected by fluorescence was constructed with a coefficient of determination, r², >0.99 for low concentrations (0.50–50 μg/ml), but tocopherols in pulses appeared to be inefficiently extracted. TAG regioisomers were quantified based on a Critical Ratio (CR), [AA]⁺/[AB]⁺, interpolated between the CRs of two pure regioisomer standards taken from alternating calibration standards. TAG mole percent relative compositions are reported for the first time for most pulses and the compositions are given with more detail and specificity than previously reported.