双肌臀大约克猪选育与杂交利用的初步研究

描述了滑刃线虫属（Aphelenchoides Fischer,1894)的2个国内新记录种：游泳滑刃线虫(Aphelenchoides hechaleos)和内卷滑刃线虫(A.involutus)。游泳滑刃线虫采自辽宁省盖州市熊岳镇莴苣(Lactuca sativa)的根际土壤中,它与近似种拟游泳滑刃线虫(A.paranechaleos)的主要区别是雌虫尾较长,尾的形状是腹面凸（而拟游泳滑刃线虫的尾较短,尾的形状是背面凸,腹面凹）,雄虫交合刺背杆的弯曲程度较小。内卷滑刃线虫采自辽宁省凌海市苍耳(Xanthium sibiricum)及山东济南大花金鸡菊(Coreopsis grandiflora)的根际土壤中,它与近似种A.jacobi的主要区别是雌虫尾向腹面弯曲程度较大,尾后部呈钩状,尾较长。     

25%丙硫克百威悬浮种衣剂对水稻干尖线虫病的防效

25%丙硫克百威悬浮种衣剂每千克种子4mL、8mL包衣水稻种子,对水稻种子的发芽、出苗没有影响,水稻苗高显著高于对照,实际产量、穗长、每穗实粒数都显著高于不用药处理,该种衣剂各处理对干尖线虫病的防效均高于95%杀螟丹可溶性粉剂6000倍浸种处理的防效。     

Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode,Aphelenchoides besseyi

RNA interference (RNAi) is a powerful tool for the analysis of gene function in nematodes. Fatty acid and retinol binding protein (FAR) is a protein that only exists in nematodes and plays an important role in their life activities. The rice white-tip nematode (RWTN), Aphelenchoides besseyi, is a migratory endoparasitic plant nematode that causes serious damage in agricultural production. In this study, the expression levels of eight RWTN genes were effectively decreased when RWTN was fed Ab-far-n (n: 1–8) hairpin RNA transgenic Botrytis cinerea (ARTBn). These functions of the far gene family were identified to be consistent and diverse through phenotypic changes after any gene was silenced. Such consistency indicates that the body lengths of the females were significantly shortened after silencing any of the eight Ab-far genes. The diversities were mainly manifested as follows: (1) Reproduction of nematodes was clearly inhibited after Ab-far-1 to Ab-far-4 were silenced. In addition, silencing Ab-far-2 could inhibit the pathogenicity of nematodes to Arabidopsis; (2) gonad length of female nematodes was significantly shortened after Ab-far-2 and Ab-far-4 were silenced; (3) proportion of male nematodes significantly increased in the adult population after Ab-far-1, Ab-far-3, and Ab-far-5 were silenced, whereas the proportion of adult nematodes significantly decreased in the nematode population after Ab-far-4 were silenced. (4) Fat storage of nematodes significantly decreased after Ab-far-3, Ab-far-4, and Ab-far-7 were silenced. To our knowledge, this is the first study to demonstrate that Ab-far genes affect sex formation and lipid metabolism in nematodes, which provides valuable data for further study and control of RWTNs.