全文获取类型
收费全文 | 2406篇 |
免费 | 135篇 |
国内免费 | 104篇 |
专业分类
电工技术 | 45篇 |
综合类 | 102篇 |
化学工业 | 684篇 |
金属工艺 | 369篇 |
机械仪表 | 133篇 |
建筑科学 | 104篇 |
矿业工程 | 34篇 |
能源动力 | 55篇 |
轻工业 | 155篇 |
水利工程 | 10篇 |
石油天然气 | 107篇 |
武器工业 | 9篇 |
无线电 | 222篇 |
一般工业技术 | 205篇 |
冶金工业 | 135篇 |
原子能技术 | 52篇 |
自动化技术 | 224篇 |
出版年
2024年 | 3篇 |
2023年 | 23篇 |
2022年 | 118篇 |
2021年 | 160篇 |
2020年 | 71篇 |
2019年 | 31篇 |
2018年 | 31篇 |
2017年 | 52篇 |
2016年 | 59篇 |
2015年 | 66篇 |
2014年 | 111篇 |
2013年 | 139篇 |
2012年 | 144篇 |
2011年 | 178篇 |
2010年 | 130篇 |
2009年 | 149篇 |
2008年 | 132篇 |
2007年 | 149篇 |
2006年 | 181篇 |
2005年 | 91篇 |
2004年 | 93篇 |
2003年 | 95篇 |
2002年 | 86篇 |
2001年 | 79篇 |
2000年 | 50篇 |
1999年 | 50篇 |
1998年 | 21篇 |
1997年 | 12篇 |
1996年 | 21篇 |
1995年 | 20篇 |
1994年 | 23篇 |
1993年 | 12篇 |
1992年 | 18篇 |
1991年 | 10篇 |
1990年 | 4篇 |
1989年 | 9篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1980年 | 3篇 |
1977年 | 1篇 |
排序方式: 共有2645条查询结果,搜索用时 343 毫秒
1.
Theodoros Eleftheriadis Georgios Pissas Marta Crespo Evdokia Nikolaou Vassilios Liakopoulos Ioannis Stefanidis 《International journal of molecular sciences》2021,22(4)
Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection. 相似文献
2.
Yu-Hung Lee Ching-Fang Yu Ying-Chieh Yang Ji-Hong Hong Chi-Shiun Chiang 《International journal of molecular sciences》2021,22(4)
The low overall survival rate of patients with pancreatic cancer has driven research to seek a new therapeutic protocol. Radiotherapy (RT) is frequently an option in the neoadjuvant or palliative settings for pancreatic cancer treatment. This study explored the effect of RT protocols on the tumor microenvironment (TME) and their consequent impact on anti-programmed cell death ligand-1 (PD-L1) therapy. Using a murine orthotopic pancreatic tumor model, UN-KC-6141, RT-disturbed TME was examined by immunohistochemical staining. The results showed that ablative RT is more effective than fractionated RT at recruiting T cells. On the other hand, fractionated RT induces more myeloid-derived suppressor cell infiltration than ablative RT. The RT-disturbed TME presents a higher perfusion rate per vessel. The increase in vessel perfusion is associated with a higher amount of anti-PD-L1 antibody being delivered to the tumor. Animal survival is increased by anti-PD-L1 therapy after ablative RT, with 67% of treated animals surviving more than 30 days after tumor inoculation compared to a median survival time of 16.5 days for the control group. Splenocytes isolated from surviving animals were specifically cytotoxic for UN-KC-6141 cells. We conclude that the ablative RT-induced TME is more suited than conventional RT-induced TME to combination therapy with immune checkpoint blockade. 相似文献
3.
Myelodysplastic syndromes (MDS) are highly heterogeneous myeloid neoplasms, and a large number of
patients are difficult to diagnose and classify by blood and bone marrow examination. As a surface marker of
granulocyte, studies have shown CD10 can be used to define the degree of granulocyte maturation in MDS patients.
However, whether it can be used for differential diagnosis of MDS and other hematological diseases remains
inconclusive. To explore the value of CD10 for differential diagnosis of MDS, 60 newly diagnosed MDS, 20 aplastic
anemia (AA) patients, and 35 iron-deficient anemia (IDA) patients were selected for this study. Bone marrow (BM)
specimens were processed for surface marker analysis and labeled with pre-conjugated monoclonal antibodies. Stained
cells were detected by flow cytometry. Our results indicated that CD10-positive granulocytes were significantly
decreased in BM of MDS patients than AA and IDA patients, and the level of CD10-positive mature granulocytes was
not associated with the clinical stages of malignancy. Receiver operating characteristic (ROC) areas under the curve
(AUC) of CD10-positive granulocytes was 0.86 and 0.85, respectively, in MDS patients than the IDA group and AA
group with good specificity and sensitivity. Further, CD10-positive granulocytes were increased after effective
treatment. In conclusion, we found the decrease in CD10-positive granulocytes has a differential diagnostic value of MDS. 相似文献
4.
5.
以一个基于 FAX/ 40 E电话语音 /传真卡技术的传真系统为例 ,重点研究 FAX/ 40 E电话语音 /传真卡技术与计算机之间的通信技术 ,并讨论有关多线程技术问题。 相似文献
6.
7.
本文提出一种与现有以太网兼容的综合话音/数据协议.描述了协议模型及实现方法.并用PASCAL语言在IBM-PC机上模拟研究了该协议的性能.模拟结果表明,该协议能满足话音的实时要求,性能良好,且数据传输性能没有明显下降.协议克服了以太网传输话音延迟不确定的缺点. 相似文献
8.
TiSiW12O40/TiO2催化合成丙酸正丙酯 总被引:2,自引:0,他引:2
研究了固载杂多酸盐TiSiW12O40/TiO2催化丙酸与正丙醇的酯化反应,探讨了催化剂用量,反应时间及醇酸摩尔比对酯收率的影响。当醇酸摩尔比为1.3:1,催化剂的量为醇酸总质量的1.5%。反应时间为1.0h,酯产率可达71.4%以上,采用该催化剂合成丙酸正丙酯不仅酯化反应温度低,反应时间较短,催化剂用量少,而且可较好地回收利用,可降低成本;因此,TiSiW12O40/TiO2是合成丙酸正丙酯的优良催化剂,具有良好的应用前景。 相似文献
9.
杨水金 《化学推进剂与高分子材料》2002,(3):5-7
综述了改性ZSM-5分子筛,有机锡,氯化铁,TiSiW_(12)O_(40)/TiO_2,CaO脱水法,锆酸酯、杂多钨锗酸等7种不同催化剂催化合成氯乙酸正丁酯的实验结果。结果表明:SO_4~(2-)/TiO_2-Al_2O_3,TiSiW_(12)O_(40)/TiO_2,SO_4~(2-)/膨润土及磷钨杂多酸14种催化剂对合成氯乙酸正丁酯的酯收率较高,具有实际应用价值。 相似文献
10.
调质状态下40Cr钢的焊接 总被引:1,自引:0,他引:1
在合理制定焊接工艺的基础上,采用R307珠光体耐热钢焊条成功地进行40Cr钢在调质状态下的焊接,解决了通常采用25—13型奥氏体焊条焊接出现的焊缝强度低、焊接接头性能差的问题。 相似文献